Acta Pharmaceutica Hungarica 63, 19-27 (1993). TLC of ethinylestradiol, mestranol, norgestrel, norethisterone on silica with toluene - ethyl acetate 2:1. Visualization by spraying with 1% cerium(IV)sulfate solution in 10 % aqueous sulfuric acid and heating at 110 °C for 5-10 min.

J. Liq. Chromatogr. Relat. Technol. 28, 1573-1581 (2005). HPTLC of cholic acid, glycocholic acid, glycodeoxycholic acid, chenodeoxycholic acid, deoxycholic acid, lithocholic acid, and glycolithocholic acid, on RP-18 W, RP-18, RP-2, and cyano phase with methanol - water, organic mixture (acetonitrile - methanol 1:1) - water, acetone - water, dioxane - water and acetonitrile - phosphate buffer (pH 4.60) in different volume compositions. Detection by spraying with a 10 % aqueous solution of sulfuric acid, or by dipping in 10 % ethanolic solution of sulfuric acid and heating at 120 °C for 20 min. Five neighbouring bile acids were separated only when cyano phase was used with acetone - water 1:1; deoxycholic acid and chenodeoxycholic acid were separated only on RP-2 plates with methanol - water 13:7.

J. Chromatogr. 411, 379-392 (1987): TLC of ecdysone 22-acyl esters on silica and ethyl-, octyl-, octadecyl- and cyano-bonded silica with solvent systems of different polarity. Detection by fluorescence quenching under UV 254 nm and by spraying with vanillin - sulfuric acid and heating at 110°C for 5 min. Evaluation of various types of TLC systems. Radioscanning.

J. Liq. Chromatogr. & Relat. Technol. 29, 2095-2109 (2006). Analytical and preparative TLC of ecdysteroids (e. g. dacryhainansterone, 20-hydroxy-ecdysone) on silica gel in unsaturated glass chambers with dichloromethane - methanol - benzene 25:5:3 and ethyl acetate - 96% ethanol - water 16:2:1. Detection under UV 254 nm or by spraying with vanillin-sulfuric acid reagent, followed by detection under white light and under UV 366 nm. Densitometric absorbance measurement at 254 nm.

Chinese J. Ethnomed. Ethnopharm. 23, 69-71 (2011). In recent years, some unauthorized specific medicines were available as “traditional cure”, “folk recipe”, or “ancestral folk prescription” in China, such as powders and pills for relieving cough or capsules for rheumatic arthritis. It has been found that certain kinds of steroid hormones were added. Description of a quick method for screening of prednisone acetate and dexamethasone acetate in the chloroform extracts of the popular medicines by TLC on silica gel with dichloromethane – diethyl ether – methanol – water 385:60:15:2. Detection by heating at 105 °C for 10 min followed by spraying with 2 % alkaline tetrazolium solution and viewing under daylight.

Pharmazie 37, 836-838 (1982). TLC of ethinylestradiol on silica with chloroform - ethyl acetate 8:2. Densitometry at 280 nm.

I. C19-steroids in axillary hair. Acta medica 40, 139-145 (1983). TLC of 5-androstene-3 beta,17 beta-diol; testosterone, 5-alpha dihydrotestosterone, dehydroepandrosterone; androsterone; 4-androstene-3, 17-dione; 5-alpha-androstane-3,17-dione on alumina with hexane - ethyl acetate - ethanol - acetic acid 120:130:1:2, and on silica with a) chloroform - dioxane 95:5, b) cyclohexane - ethyl acetate 8:2, c) chloroform - acetone 3:2.

J. Planar Chromatogr. 1, 161-167 (1988). Two-dimensional TLC on four types of layer with nine solvents for normal phase TLC and four solvents for reversed-phase TLC. Presentation of the optimal combinations. Evaluation of 171 computer-generated systems for the separation of a mixture of 15 steroids using visual inspection, the distance between the most poorly separated pair of solutes and the value of each three chromatographic optimization function as criteria for separation quality.