J. Planar Chromatogr. 31, 332-336 (2018). HPTLC of β-sitosterol (1) and lupeol (2) in Saussurea lappa and Saussurea auriculata on silica gel with toluene ‒ ethyl acetate ‒ glacial acetic acid 29:9:2. Detection by spraying with anisaldehyde–sulfuric acid reagent. Quantitative determination by absorbance measurement at 525 nm. The hRf values for (1) and (2) were 71 and 88, respectively. Linearity was between 100 and 400 ng/zone. LOD and LOQ were 7 and 23 ng for (1), and 6 and 17 ng for (2), respectively. The intermediate precision was <2 %. Average recovery was 99.9 % for (1) and 100.0 % for (2).

J. A.O.A.C. 70, 499-501 (1987). TLC determination of coprostanol and cholesterol on silica, impregnated with 5 % alcoholic phosphomolybdic acid solution, with ether - heptane 55:45. Detection by heating for 20 min at 120 °C.

J. Liquid Chromatogr. 12, 3151-3161 (1989). TLC of sterols and other lipids on silica with isopropyl ether – acetic acid 96:4, followed by hexane – ether – acetic acid 90:10:1. Detection of lipids by spraying with 5% ethanolic phosphomolybdic acid and heating for 5-10 min at 100-120°C. Semiquantification by comparison of the zone sizes and intensities of standards and samples. Quantification of cholesterol by densitometry after development with chloroform – ethyl acetate 96:4, and dipping in cupric acetate – phosphoric acid reagent and heating at 100°C. Quantification of triacylglycerols and free fatty acids by densitometry after development with hexane – ether – acetic acid 80:20:1, followed by spraying with cupric acetate – phosphoric acid reagent. Also GC.

Anal. Chim. Acta 275, 177-182 (1993). HPTLC of corticosteroids on silica with chloroform - methanol 98:2. Detection by spraying with a mixed solution of 1% sulfuric acid in acetic acid, and heating for 10 min. at 95 °C. Examination in daylight and under UV 366 nm.

J. Liqu. Chromatogr. 23, 282-294 (2000). Comparison of techniques TLC of 20-hydroxyecdysone and polypodine B (5,20-dihydroxyecdysone) on silica gel with dichloromethane - ethanol (96%) 4:1, chloroform - methanol - benzene 25:5:2, and ethyl acetate - methanol - conc. NH3 17:2:1. Detection directly after development under UV 254 nm; quantitation by densitometry at 254 nm in reflectance mode. - Parallel analyses using both TLC with densitometry and HPLC gave similar results.

Acta Chrom. 13, 95-101 (2003). Chromatographic systems comprising different stationary and mobile phases were investigated for determination of oxysterols in plasma by TLC. Two chromatographic systems are used to ensure selectivity. TLC on RP-18 with 2-propanol – dichloromethane 3:97, or on silica gel with acetone – chloroform 1:9 in a horizontal chamber. Detection by spraying with Liebermann – Burchard reagent (methanol - conc. H2SO4 - acetic anhydride, 10:1:1) followed by heating at 110 °C for 5 min. Densitometry at 366 nm (oxysterols) and 254 nm (steroid hormones).

Collaborative study. J. Chromatogr. 452, 399-408 (1988). Comparison of quantitative analysis of bile acids in biological samples with other methods. Discussion of the specificity, sensitivity, accuracy and simplicity of each. TLC gives reliable results for biliary bile acids except for differentiation between conjugates dihydroxycholanoic acids. The method compared were enzymatic analysis, radioimmunoassay, GC, HPLC and GC/MS.

J. Liq. Chrom. & Rel. Technol. 26, 2741-2750 (2003). HPTLC of bile acids (cholic, chenodeoxycholic, deoxycholic, lithocholic, glycocholic, glycodeoxycholic, and glycolithocholic acid) on RP-18 with mixtures of methanol - water. The methanol content was varied by 5% volumes from 60% to 100%. Detection after drying at room temperature by spraying with a 10% solution of sulfuric acid in water and heating at 120°C for 20 min. The retention parameters RMW may be used as a measure of lipophilicity of the investigated bile acids.