J. Chromatogr. 367, 323-334 (1986). TLC of some rifamycins and steroids on diphenyl-bonded silica with various solvents, including non-aqueous and aqueous binary mixtures. Proposal of a dual retention mechanism. Interactions of the residual silanol groups seem to prevail when using lipophilic solvents as the mobile phase, whereas interactions with bonded aryl. groups dominate when high polarity alcoholic or aqueous mixtures are being used. Diphenyl silica precoated plates, rifamycins, steroids, mobile phase composition

J. Agric. Food Chem. 38, 1667-1673 (1990). Two step-TLC on silica with toluene - methanol 1:1 for 5 cm, after drying with toluene to 16 cm. Detection of tri-, di- and monoacylglycerols and free fatty acids by exposing to iodine vapor; of cholesteryl esters, cholesterol, cholesterol oxides and phospholipids by spraying with 50% sulfuric acid and heating at 110°C for 5 min. Quantification by determining the 14C activity.

J. Planar Chromatogr. 5, 251-354 (1992). TLC of 21 amides of 11a-hydroxyprogesterone 11-hemisuccinates on RP-2, RP-8 and RP-18 silica with 3 different eluent systems (aqueous methanol (pH ca. 8); aqueous methanol adjusted to pH 1 with HCl; methanol in 0.01 M phosphate buffer). Detection under UV 254 nm. In addition study of the correlation between structure and retention.

Analyst 119, 2571-2575 (1994). TLC on silica with 1) hexane - ether - dichloromethane 5:9:6, 2) chloroform - acetone 9:1, 3) cyclohexane - ethyl acetate - ethanol 24:16:1, 5) toluene - methanol - water 1:15:4, 6) hexane - dichloromethane - acetonitrile 8:2:1. Detection by spraying with N-methyl-N-(trimethylsilyl)-trifluoroacetamide solution and viewing under UV 366 nm. Identification by comparison of Rf values and fluorescence colors.

CBS 101, 5-7 (2008). HPTLC of steroids on RP-18W and RP-18 with methanol - water 3:2 in a flat bottom chamber. Detection by spraying with perchloric acid (20 % in ethanol) followed by heating at 100 °C for 5 min. Migration time on the hydrophobic RP-18 layer was 130 min whereas on the water-wettable RP-18W layer it was 39 min. The maximal water content of the mobile phase is 40 % for RP-18 layers and up to 100 % for RP-18W. Separation of steroids was better on RP-18W. The hRf value of stanozolol was 4, of methyl testosterone 12, of Reichstein’s S 26, of hydrocortisone 37 and cholesterol remained at the application position.

J. of Medicinal Chemistry 32, 203-213 (1989). TLC on silica with ether - petrol ether 1:1, chloroform - ether - methanol 90:8:2 and petrol ether - ethyl acetate - methanol 50:45:5. Visualization by UV; iodine vapor or by immersion in 50% phosphoric acid and heating for 30 min. at 120°C.

J. Planar Chromatogr. 6, 228-231 (1993). OPLC of potassium canrenoate, canrenoic acid and canrenone on silica with different mixtures of methanol and 0.01 M phosphate buffer (pH 5.0). Quantification by densitometry at 285 nm.

J. Planar Chromatogr. 26, 375-378 (2013). TLC of estrone, estradiol, and estriol on silica gel with chloroform - ethyl acetate - acetone 6:2:1. Detecion by dipping into (1) 10 % solution of phosphomolybdic acid (PMA) in methanol, followed by heating at 100 ºC for 10 min; (2) 0.2 % ceric ammonium sulfate in phosphoric acid, followed by heating at 110 ºC for 10 min; (3) 0.2 g manganese(II) chloride in 30 mL water, 30 mL methanol and 2 mL concentrated sulfuric acid, followed by heating at 100-120 ºC for 10-15 min; and (4) 1 g of vanillin in 25 mL of ethanol, 25 mL of distilled water, and 35 mL of ortho-phosphoric acid (85 %), followed by heating at 120-160 ºC for 5-15 min. The lowest detection limit for estrone (75 ng/zone) was achieved using (1) and (3), whereas for estradiol and estriol (both 4.7 ng per zone) by using (2).