Phytochemistry 23, 2925-2929 (1984). Isolation of sterols from a crude extract of cucurbita maxima seeds by PLC on silica with ether - benzene 1:9 (4 developments). Detection by UV after spraying with 0.05 % solution of rhodamine 6G in acetone. Method suitable for the separation of delta 5-sterols from delta 7-sterols. See also: V. Garg and W. Nes, Phytochemistry 23, 2919 (1984).

Biochemical Systematics and Ecology 15, 639-641 (1987). TLC on argentated silica with benzene - hexane 1:1, two runs.

Biochemical Systematics and Ecology 19, 17-24 (1991). Isolation of sterols after saponification by TLC on silica with toluene - isobutanol - ethanol - acetone 25:20:2:1. Detection by spraying with Rhodamine 6G solution.

(Hungarian). Acta Pharmaceutica Hungarica 64, 171-174 (1994). TLC of steroids on silica with ethyl acetate - chloroform 1:9. Visualization by exposure to iodine vapor, under UV 365 nm or by spraying with 50% phosphoric acid and heating for 10 min.

Bol. Soc. Chil. Quim. 47, 61-66 (2002). HPTLC of cholesterol, triolein and phosphatidylcholine on silica gel by AMD with a gradient from chloroform - methanol - water 65:35:4 to diethylether - n-hexane 1:1 to n-hexane in 15 steps. Visualization by dipping in manganese(II) chloride reagent and heating at 110°C for 10 min. Quantification by densitometry at 366 nm/>400 nm. Recovery was found to be 99,4 %, repeatability and intermediate repeatability resulted adequate. Limit of detection (quantification) was 6,9 (21,2) ng for phosphatidylcholine, 2,6 (4,0) ng for cholesterol and 3,4 (8,7) ng for triolein.

J. Planar Chromatogr. 28, 380-385 (2015). HPTLC of taurocholic acid (1), glycodeoxycholic acid (2) and taurodeoxycholic acid (3) in ten Thai edible plants on silica gel with chloroform - methanol - acetic acid 7:2:1. Quantitative determination by absorbance measurement at 366 nm. Linearity was in the range of 34-2200 ng/zone for (1), 33-2100 ng/zone for (2) and 30-1900 ng/zone for (3). LOD and LOQ were 10 and 50 ng/zone for (1), 20 and 40 ng/zone for (2) and 10 and 50 ng/zone for (3). The intermediate precision was below 1.2 % (n=6). Average recovery for (1) to (3) was 99, 100 and 100 %, respectively.

J. Chromatogr. 497, 139-146 (1989). TLC of faecal bile acids on silica with isooctane - 2-propanol - acetic acid 30:10:1 for the 1st development and isooctane - ethyl acetate - acetic acid 10:10:2 for the 2nd. Detection by dipping into 0.2% 2,7-dichlorofluorescein ethanol solution. Quantification by fluoro densitometry at 366 nm. Good correspondence between this method and GC.

IV. Comparison of separation of studied bile acids by the use of cluster analysis. J. Liq. Chrom. & Rel. Technol. 27, 2987-2995 (2004). TLC of bile acids (cholic, glycocholic, glycolithocholic, deoxycholic, chenodeoxycholic, glycodeoxycholic, and lithocholic acid) on silica gel and a mixture of silica gel and Kieselguhr with n-hexane - ethyl acetate - acetic acid in various volume compositions. Detection with 10 % aqueous sulfuric acid followed by heating at 120 °C for 20 min. On silica gel the separation of glycocholic from glycodeoxycholic acid was difficult, on the mixed silica gel - Kieselguhr layer the separation of cholic acid from glycolithocholic acid. The obtained results indicate that similar analysis can be an alternative method for the estimation of chromatographic separations of studied bile acids.