Juss) as determined by a combination of chromatographic and spectral techniques. J. Liq. Chromatogr. & Relat. Technol. 30,11-25 (2007). TLC on silica gel with petroleum ether - acetone 25:2; detection by spraying with 50 % ethanolic sulfuric acid and heating at 200°C. Isolation, purification and quantification of lipid classes by preparative TLC; detection by spraying the edges with 2’,7’-dichlorofluorescein for visualization under UV. Preparative separation of acylglycerols, free fatty acids, and polar lipids on silica gel with petroleum ether - acetone - acetic acid 70:30:1. Quantitative Ag-TLC on silica gel impregnated by dipping into a 0.5 % methanolic silver nitrate solution - also preparative Ag-TLC. Quantitative TLC on RP by densitometry at 450 nm. Ag-TLC provided the quantitative data for the TAG classes differing in unsaturation, and RP-TLC for the TAG species differing in chain length within a given class.

J. Agric. Food Chem. 56, 7644-7648 (2008). HPTLC of ferulic acid from agricultural waste (maize bran, rice bran, wheat bran, wheat straw, sugar cane baggasse, pineapple peels, orange peels and pomegranate peels) on silica gel with benzene - dioxane - acetic acid 85:15:1. Detection under UV 254 nm. Purification by adsorption chromatography followed by preparative layer chromatography.

CBS 106, 7-10 (2011). HPTLC of sucralose on silica gel (pre-washed by development with methanol, followed by drying at 100 °C for 15 min) with isopropyl acetate – methanol – water 15:3:1 up to 60 mm (migration time 15 min). Detection by dipping in aniline diphenylamine o-phosphoric acid reagent followed by heating at 120 °C for 20 min, evaluation under white light and UV 366 nm. Quantitative determination by absorbance measurement at 400 nm. Via the TLC-MS Interface the respective zones were eluted and transferred into a single-quadrupole mass spectrometer. Electrospray ionization mass spectra were recorded in full scan mode. The recovery of sucralose in drinking water was 84 ± 7 % (n=3). The limit of detection was 6 ng/band. The calibration curve (10-300 ng/band, r=0.9999, 1.3 %RSD) was suited to analyze sucralose at concentrations of 0.1-5 µg/L.

Food Sci. Technol. Campinas. 35, 598-604 (2015). TLC of phosphatidylglycerol (1), phosphatidic acid (2), phosphatidylethanolamine (3), phosphatidylinositol (4), phosphatidylcholine (5) and lysophosphatidylcholine (6) in the seeds of flaxseed (Linum usitatissimum) on silica gel with chloroform - acetone - methanol - acetic acid - water 10:4:2:2:1. Detection by exposure to iodine vapours and evaluation in daylight. Solid phase extraction was performed to characterize the phospholipid composition during flaxseed development.

J. Planar Chromatogr. 30, 440-452 (2017). For electroseparation experiments in a commercially available, open-air electrophoresis box, thirteen types of separation layers (filtrating paper, office paper, chromatography paper, Japanese paper for aircraft paper models, potato starch on cellulose support, common HPTLC glass plates coated with cellulose, silica gel, RP-18W, and aluminum oxide as well as glass-based nutrient agar layers) were investigated. The best separation was observed for the cellulose pre-coated TLC layer and the starch layer on filtrating paper support. The study revealed substantial differences between the electrophoretic migration of target dyes within cellulose type layers and also in comparison to the remaining stationary phases studied. Combined planar electrophoresis–electrochromatography of methyl red and ponceau R colorants on active thin-layers composed of starch powder on cellulose strips and agar layer on glass-based support connected with electrolyte containers. The resulting dye pattern on active layers was acquired using an office scanner.

Lipids 18, 87-89 (1983). The chromatographic mobilities of 17 sterols and squalene on reversed-phase TLC plates with 4 nonaqueous solvent systems is described. Solvent Systems: hexane - ethyl acetate 9:1 and other systems were used. Visualization: 10 % phosphomolybdic acid in 95 % ethanol, followed by heating at 100 °C; iodine vapor; 10 % sulfuric acid in 50 % methanol, followed by heating at 100 °C.

J. Agric. Food Chem. 32, 1254-1256 (1984). Isolation of five propylphenols with chromatographic methods, i.e. TLC on silica with ether - hexane 25:75, acetone - chloroform 5:95, methanol - ethyl acetate - chloroform 1:10:89. Detection by Cladosporium TLC bioassay.

J.A.O.A.C. 68, 839-842 (1985). TLC of mycotoxins with 2-dimensional chromatography using various solvent combinations on silica. Detection by viewing under longwave UV followed by using various spray reagents, i.a. 3-methyl-2-benzothiazoline hydrazone hydrochloride solution, Pauly 5 reagent, ethanolic aluminium chloride, Ehrlichs reagent, sulfuric acid, etc. Patulin, kojic acid, penicillic acid, sterigmatocystin, cyclopiazonic acid, trichothecenes, tenazonic acid.