J. Planar Chromatogr. 17, 328-334 (2004). HPTLC of eighteen pesticides (propaquizafob, quizalofop-P, triadimefon, triadimenol, dimethomorf, quinoxyfen, cyromazine, oxyfluorfen, fluoroglycofen, acetochlor, metazachlor, imazapyr, furalaxyl, triclopyr, buprofezin, pyriproxyfen, fenoxycarb, piperonyl butoxide) on cyano phase. The greatest spread of separated compounds was obtained by combining nonaqueous normal-phase mobile phases (tetrahydrofuran or ethyl acetate in n-heptane 1:4 in the first direction and aqueous reversed phases mobile phases (methanol - water 7:3 or acetonitrile - water 1:1) in the second dimension. Detection under UV light at 254 or 366 nm. Videoscanning and densitometry at 254 nm.

J. AOAC Int. 90, 857-863 (2007). TLC of azadirachtin on silica gel with dichloromethane - ethanol 20:1. Detection by spraying with acidified vanillin reagent (3 g vanillin in 10 mL ethanol with 1.5 mL concentrated sulfuric acid) followed by heating at 110 °C for 3 min. Quantitation by densitometry.

J. Planar Chromatogr. 20, 13-18 (2007). Graft TLC separation of 28 pesticides (aziprotryne, fenvalerate, desmetryn, terbutryn, pyriproxyfen, benzthiazuron, fluoroglycofen-ethyl, bensultap, benalaxyl, thiabendazole, metalaxyl, tetramethrin, imazalil, atrazine, chlorfenvinphos, methoxychlor, carbaryl, alachlor, bromopropylate, captan, diuron, tetradifon, napropamide, metribuzin, metamitron, p,p’-DDE, dinoseb, monolinuron) on connected layers - silica and octadecyl silica wettable with water, achieved by two dimensional planar chromatography using a non-aqueous mobile phase in the first dimension and an aqueous reversed-phase mobile phase in the second dimension. HPTLC on silica gel with 1) ethyl acetate - n-heptane 1:4 or 3:7 in the first dimension and, after cutting into strips, connection with RP 18 plates and transfer with methanol, with 2) methanol - water 3:2 or 3:1 in the second dimension. Detection under UV light at 254 or 366 nm.

J. Planar Chromatogr. 22, 457-458 (2009). TLC of monocrotophos and biological extracts, dimethoate, endosulfan, carbaryl, and cypermethrin on silica gel with chloroform - acetone 7:3 with chamber saturation. Detection by spraying with 5 % sodium hydroxide solution followed by 5 % benzil reagent (5 g benzil in 100 mL acetone) and heating at 100 °C for 10 min. Monocrotophos was detected as a pink zone in daylight.

Anhui Agri. Sci. Bull. 18 (1), 43-45 (2012). Soybean saponin is a component of soybean which has physiological activities and is composed of a variety of monomers. Presentation of a procedure for the separation of saponin extracted from soybean using ethanol. TLC on silica gel with 1) chloroform – ethyl acetate – methanol – water 15:40:22:10; 2) chloroform – ethyl acetate – methanol – water 3:4:2:1; 3) chloroform – ethyl acetate – methanol – water – acetic acid 3:4:2:1:0.05; 4) chloroform – ethyl acetate – methanol – water – acetic acid 30:80:40:20:1. Detection by spraying with 10 % sulfuric acid in ethanol and heating at 95 ºC until the zones are clearly visualized. Detection under UV 366 nm. The results showed that the mobile phase 1 gave the optimum separation of the soybean saponin.

J. of Chromatogr. Sci. 53 (2), 338-344 (2014). Presentation of a method for the simultaneous quantification of three glycosidic isoflavones (daidzin, genistin and glycitin) in soybean (Glycine max L.) by HPTLC on silica gel with toluene – ethyl acetate – formic acid – acetic acid 2:16:2:1. The hRf values of daidzin (1), genistin (2) and glycitin (3) were 39, 51 and 32, respectively. Detection and quantification by densitometry at 260 nm. Validation in accordance with the ICH guidelines with the results for precision of ≤2.1 %, ≤0.7 % and ≤0.1 %, recovery of 95.9-106.7 %, 86.9-106.6 % and 98.5-105.6 %, LOD of 3, 19 and 4 µg/mL and LOQ of 9, 59 and 11 µg/mL for the glycosidic forms of (1), (2), and (3). The method was used for the analysis of the soybean variety Kh-09 bragg which showed high amounts of glycosidic isoflavones: 278, 598 and 109 µg/g for (1), (2), and (3). After fermentation with Bacillus subtilis, the concentration of glycosidic isoflavones significantly decreased while those of the aglycone isoflavones increased.

J. Chromatogr. A 1507, 124-131 (2017). Fast HPTLC-fluorescence detection (HPTLC-FLD) screening of the total ergot alkaloids in rye using lysergic acid amide (LSA) as chemical marker. Generally these substances are determined by HPLC-FLD or mass selective detection, analyzing the individual compounds. For analysis by HPTLC-FLD, transformation of the ergopeptine alkaloids selectively to LSA after an ammonium acetate buffered extraction step followed by liquid-liquid partition for clean-up. HPTLC on silica gel with isopropyl acetate – methanol – water – 25 % ammonium hydroxide solution 800:100:38:11. Quantitative determination using the enhanced native fluorescence of LSA and ergometrine at 313/>400 nm without any interfering matrix. The LOD and LOQ were 8 and 26 μg/kg LSA in rye, which enables the determination of the total ergot alkaloids far below the limit for rye. Recoveries were close to 100 % for different rye flours at relevant spiking levels. The screening method proved to be reliable, fast and efficient for the total ergot alkaloids in rye, and is a rapid alternative to the HPLC analysis of the individual compounds.

J. Agric. Food Chem. 33, 95-98 (1985). TLC determination of 4-chlorobenzotrichloride and metabolites on silica with hexane - ethyl acetate - acetic acid 2:1:0.1, hexane - acetic acid 10:1, hexane - ether 5:1 and hexane.