J. AOAC Int. 86, 602-611 (2003). Review of the advances in the applications of TLC and HPTLC for the separation, detection, identification, and determination of pesticides, other agrochemicals, and related compounds for the period 2000-2002. Analyses are described for a variety of samples, such as food, biological, and environmental samples, and for residues of pesticides of various types, including insecticides, herbicides, and fungicides, belonging to different chemical classes. 88 references are included for residue analysis, hydrophobicity studies, and the use of TLC and thin-layer radio-chromatography for studies of pesticide metabolism, degradation, uptake, and related topics.

J. Planar Chromatogr. 19, 361-370 (2006). Log P, log kW, and phi 0 are proposed and compared as descriptors of the hydrophobicity of twenty-two dihydroxy-thiobenzanilides. Chromatographic data log kW and phi 0, were calculated from experimental TLC results obtained on RP-18W and cyano phases with methanol or acetone as organic modifiers. The calculated hydrophobicity was compared with the biological activity of the test substances. Development in saturated sandwich chambers at 20 °C. Detection at 320 nm.

J. Planar Chromatogr. 21, 161-166 (2008). TLC of 13 newly synthesized potential herbicides (N-aryltrichloroacetamides or 2-(chlorophenoxy)acyl derivatives) on silica gel or RP-18 in saturated sandwich chambers at 20 °C with hexane - 1,2-dichloroethane 2:3. Detection in UV light at 254 nm. The lipophilicity of the substances was described by retention factors in water, log k w, both calculated from experimental RP-TLC data, and by log P values calculated by use of software. Biological activity was determined with the BioArena system. Antibacterial action against Pseudomonas savastanoi pv. phaseolicola bacterial cells and the role of formaldehyde were investigated. Additionally the effect of formaldehyde capturers (L -arginine and reduced glutathione) and Cu 2+ ions was evaluated in regard of bioactivity and toxicity. Formaldehyde and its reaction products are crucial in the action mechanism of these substances.

J. Liq. Chromatogr. Relat. Technol. 33, 948-956 (2010). HPTLC of triazine herbicides (atraton, terbumelon, simazine, and terbutylazine) on silica gel with methyl-t-butyl ether - cyclohexane 1:1 in a vertical developing chamber without chamber saturation. Detection by derivatization using chlorine and starch-iodine. Quantitation with a CCD camera; the range of linearity covers two magnitudes (for atrazine from 10 ng to 1000 ng).

CBS 112, 5-7 (2014). HPTLC-AMD of sediment samples and standards 17α-ethinylestradiole, 17ß-estradiole, estrone and bisphenol A on silica gel with a two step gradient, first step with methanol to 20 mm, second step with ethyl acetate - n-hexane 1:1 to 80 mm. Detection with the p-YES-bioassay by dipping in yeast cell suspension (the cell number of the genetically modified yeast strain (Saccharomyces cerevisiae BJ3505) was determined by photometry and diluted to 150 ± 50 Formazin Attenuation Units) followed by incubation for 3 h at 30 °C in a saturated steam atmosphere. Then the enzyme substrate solution (0.5 mg/mL 4-methylumbelliferyl-ß-D-galactopyranoside solution) was sprayed on the TLC plate followed by a second incubation for 30 min at 37 °C. Detection under UV 254 nm and quantitative fluorescence measurement at UV 320/>400 nm. Two blue fluorescent zones with estrogenic activity were detected at hRF 65 and 80.

J. Chromatogr. A 1468, 228-235 (2016). Development and validation of a rapid and simple screening method by HPTLC for antioxidant activity in samples of 16 algal species collected from local Victorian beaches. Quantification of fucoxanthin, one of the most abundant marine carotenoids directly from the HPTLC plates before derivatization either with 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical or ferric trichloride to analyze antioxidants in marine algae, based on their ability to chelate iron ions or to scavenge non-biological stable free radical, respectively. Classification of algae species into 5 groups according to the chemical/antioxidant profiles by principal component analysis of obtained HPTLC fingerprints. The investigated brown algae samples were rich in non-polar and moderate-polar and phenolic compounds with antioxidant activity, while most of the phenolic iron chelators showed free radical scavenging activity. Strong positive and significant correlations between total phenolic content and DPPH radical scavenging activity showed that phenolic compounds, including flavonoids are the main contributors of antioxidant activity in these species, which could be considered for future applications in medicine, dietary supplements, cosmetics or food industries. Discussion of the advantages of the proposed methods in terms of screening for antioxidants and quantification of antioxidant constituents in complex mixtures using the flexible and versatile standard HPTLC system in the drug discovery, and the possibility of using the method for the bioassay-guided isolation of unknown natural antioxidants and subsequent identification if combined with spectroscopic identification.

(Spectrophotometric determination of afugan in plant products.) Mikrochimica Acta (Wien) 1, 393-398 (1984). TLC of the fungicide afugan on silica with hexane - acetone 8:1. Detection by UV 254 nm. Spectrophotometric determination at 254 nm.

J.A.O.A.C. 68, 458-461 (1985). TLC of aflatoxins B1, B2 and M 1 on silica with chloroform - acetone 9:1 or ether - methanol - water 94:4.5:1.5 for aflatoxins 1B, B2, G1 and G2, and chloroform - isopropanol - acetone 85:5:10 for aflatoxin M1. Quantification by densitometry. Comparison with other methods. This method is more efficient with respect to recovery and shorter analysis time.