CBS 117, 11-12 (2006). For the rapid control of the fish species escolar (Lepidocybium flavobrunneum) by determination of the indigestible wax esters, HPTLC on silica gel with n-hexane – toluene 7:3 with chamber saturation for 10 min, migration distance 60 mm. Detection by dipping in aqueous Rhodamine B reagent (0.025 %), evaluation under UV 366 nm. Quantitative determination by fluorescence measurement at 366/>400 nm with the Hg lamp. The hRF value of oleyl oleate was 30 and of stearyl stearate 40. The mean recovery rate (104 %) was determined by analyzing salmon samples spiked to contain 20 % wax esters. The wax ester content of an escolar sample was 18-22 % (n=3). The repeatability was <5 % and the mean laboratory precision 3 %.

Lipids 17, 650-655 (1982). For preparative separation silica plates were used with hexane - ether 6:4. For separation of hydrogenated oils into their various components, multiple development (4 or 5x) TLC was carried out with ether - benzene 1:99 followed by ether - benzene 3:97. Analytical TLC components were visualized by charring with sulfuric acid-dichromate or with iodine vapors. reparative TLC: bands were located by spraying with ethanolic dichlorofluorescein, followed by viewing under UV. The components of the seed oil of trewia nudiflora and mallotus phillipinensis were chromatographed and compared.

V. Danube Symposium on Chromatography, Yalta, November 11.-16., 1985. TLC of phospholipids on silica with chloroform - methanol - water 65:25:4, identification of phospholipids using Bartlet's method.

J. Chromatogr. 349, 295-300 (1985). Study on the interaction of the membrane phospholipid, dioleylphosphatidyl choline with 23 nonionic tensides by charge-transfer. Reversed-phase TLC on cellulose with water - ethanol 12:13. Exposure of the chromatogram to iodine vapor. Discussion of the dependence of the lipophilicity of dioleoyl phosphatidyl choline on tenside concentration of eluent.

J. Chromatogr. 365, 229-235 (1986). Preparative isolation of different lipid classes by HPLC on silica column using a multistep gradient. HPTLC (linear, circular and anticircular) of the fractions on silica with hexane - diethyl ether - acetic acid 65:14:1 for neutral lipids, and chloroform - methanol - water 65:25:4 for polar lipids. Identification by CC/MS.

J. Liquid Chrom. 10, 1687-1705 (1987). TLC after acidic, alkaline or enzymatic hydrolysis on silica with preadsorbent zone. Predevelopment 3x with chloroform/methanol 1:1 on the preadsorbent zone, then actual development with chloroform - ethanol - triethylamine - H2O 30:34:30:8. Detection with 10% coppersulfate in 8 % H3PO4, or with 0.2 % ninhydrin in acetone after heating for 2 min. at 170 °C. Quantification by absorbance at 400 nm.

Evidence for polyphosphoinositide metabolism inside the nucleus which changes with cell differentiation. Biochem. J. 248, 765-770 (1987). TLC of lipid extracts on silica sprayed with 1% oxalate with chloroform - methanol - water - NH3 45:35:8:2, or on silica sprayed with 1mM NaEGTA (pH 5.5) with chloroform - methanol - acetic acid - water 65:50:2:5. Detection by autoradiography and exposure to iodine vapor.

Phytochemistry 28, 2943-2348 (1989).TLC of lipids and fatty acids on silica with ethyl acetate - isopropanol - chloroform - methanol - 0.25% aqueous KCl 25:25:25:10:9. Visualization with Cu(OAc)2 reagent. Visualization of fatty acids by spraying with 2',7'-dichlorofluorescein and viewing under UV.