J. Planar Chromatogr. 3, 55-60 (1990). A strategy for the optimization of chromatographic conditions in TLC-separation of the lipid classes in lecithins of egg yolk, rapeseed, and soybean origin was developed. This approach utilizes factorial design in sequential blocks to screen the variables (in this work, 15 in number), principal component analysis to map the experimental domain, and partial least squares regression for the final optimization. Chromatography on silica, postchromatographic derivatization with 3% cupric acetate in 8% o-phosphoric acid heated at 180°C for 8 min, substances appear as brownish spots. Scanning by absorbance at 350 nm.

Proc. 6th Int. Symp. Instrum. Planar Chromatogr., (Interlaken 1991), Inst. Chromatogr., Bad Dürkheim, FRG, 301-309 (1991).HPTLC on silica with chloroform - methanol - 0.025% aqueous CaCl2 60:40:9. Covered with a polyvinylidendifluoride (PVDF) membrane negatively loaded gangliosides were electrotransfered to the PVDF-membrane. On the membrane visualization (with resorcinol reagent) or immunoblotting.

Phytochemistry 31, 1961-1967 (1992). Separation of different lipid classes by two-dimensional TLC on silica with 1) chloroform – methanol – water 65:25:4 and 2) chloroform – acetone – methanol – acetic acid 10:4:2:1. Detection by spraying with 10% primuline in aqueous 80% acetone and under UV.

Phytochemistry 34, 1323-1333 (1993). 2D-TLC of more than 100 species representing all the 16 orders of brown algae (Phaeophyceae) on silica with 1) chloroform - methanol - water 65:25:4 and 2) chloroform - methanol - isopropylamine - conc. NH3 130:70:1-10. Detection under UV 366 nm after spraying with dichlorofluorescein (0.05% in ethanol). For the detection of betaine lipids the plate was sprayed with Dragendorff's reagent.

J. Liquid Chromatogr. 18, 527-535 (1995). HPTLC of cholesterol esters, triacylglycerols, free fatty acids, and cholesterol on silica with petrol ether (37.5 - 52 °) - ether - acetic acid 80:20:2, and hexane - petrol ether - ethyl ether - acetic acid 50:20:2:1. Detection by spraying with 5% phosphomolybdic acid in ethanol and heating at 110-120 °C for 5-10 min. Quantification by densitometry at 700 nm.

J. Planar Chromatogr. 10, 308-310 (1997). TLC of diacylglycerols, cholesterol, free fatty acids and triacylglycerols on silica with hexane - ether - acetic acid 80:20:1. Detection by spraying with three different reagents: I. 3% cupric acetate in 6% aqueous phosphoric acid solution, followed by heating at 100°C for 10 min; II. 35% aqueous sulfuric acid solution, followed by heating at 140°C for 10 min; III. procedure I. followed by procedure II. Densitometry at 430 nm (absorbance). Also published in Proc. 9th Internat. Symp. Instr. Chromatogr., Interlaken, April 9.-11., 331-335 (1997).

J. Lipid Res. 38, 1482-1488 (1997). TLC of phospholipids and neutral lipids on EDTA-impregnated silica gel and after pre-concentration with chloroform - methanol - water 60:40:10 with five step-wise developments: i) chloroform - methanol - water 65:40:5 to 2 cm, ii) ethyl acetate - 2-propanol - ethanol - chloroform - methanol - 0.25% KCl 35:5:20:22:15:9 to 5 cm, iii) toluene -diethyl ether - ethanol 60:40:3 to 7.5 cm, iv) n-heptane - diethyl ether 94:8 to 10.5 cm, v) pure n-heptane to 12.5 cm. Charring by dipping in a solution of 10% cupric sulfate in 8% phosphoric acid for 10 s and heating at 200°C for 2 min. Quantitative determination with an image analyzer in transmission mode.

J. Lipid Res. 41, 142-147 (2000). 2-D TLC of lysophospholipids (lysophosphatidic acid, lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylglycerol, lysophosphatidylinositol, lysophosphatidylserine, sphingosylphosphorylcholine, sphingosine-1-phosphate) on silica gel in the first direction with 1) chloroform - methanol - formic acid - water 60:30:7:3 and in the second direction with 2) chloroform - methanol - 28% NH3 - water 25:29:4:1. To separate lysophospholipids from neutral lipids on the same plate, a third run was performed with ether in the opposite direction to the second run.