Proc. Intern. Symp. on Planar Separations Plan. Chrom. 267-273 (2003). TLC of phospholipids (i.a. phosphatidylinositols, -cholines, -serines, -ethanolamines, -glycerines, neutral lipids) on silica gel with chloroform - methanol - water 65:25:4 and chloroform - methanol - acetic acid 65:25:8. After drying 5% phosphomolybdic acid was used for spraying or immersion; also iodine vapor, Dragendorff, butanolic ninhydrin solution and ammoniacal silver nitrate solution. Quantitation by densitometry. Effective quantitative analysis.

J. Liq. Chromatogr. & Relat. Technol. 29, 2167-2180 (2006). HPTLC of neutral lipids on prewashed silica gel with petroleum ether - diethyl ether - glacial acetic acid 80:20:1; and of methyl and steryl esters with hexane - petroleum ether (35-60°C) - diethyl ether - glacial acetic acid 50:25:5:1 in a twin trough chamber saturated for 20 min. Detection by spraying with a 50 % solution of phosphomolybdic acid in ethanol, followed by heating at 115°C for 10 min. Polar lipids were separated with chloroform - methanol - water 65:25:4. Detection by spraying with 10 % cupric sulfate solution, followed by heating at 140 °C for 10 min. Quantitative determination by absorbance measurement at 610 nm for neutral lipids and at 370 nm for polar lipids.

fruits. Indian Drugs 45(11), 908-910 (2008). HPTLC of embelin in ethanolic extract of dried fruits of Embelia ribes Burm. on silica gel with ethyl acetate - methanol 9:1. Quantitative determination by absorbance measurement at 365 nm. The total content in Embelia ribes fruit was 0.034 %. The proposed HPTLC method provides a good resolution of embelin from other constituents present in the ethanolic extract of dried fruits of Embelia ribes Burm.

Microbiol. Res.166, 87-98 (2011). Two-dimensional TLC of phospholipids from Legionella bozemanae on chloroform - methanol - water 14:6:1 in the first dimension and chloroform - methanol - glacial acetic acid 13:5:2 in the second dimension. Lipids were detected by spraying with concentrated sulfuric acid or by exposure to iodine vapor. The following phospholipids were identified: phosphatidylcholine, phosphatidyl-N,N-dimethylethanolamine, phosphatidylethanolamine, phosphatidyl-N-monomethylethanolamine, phosphatidylglycerol and diphosphatidylglycerol.

J. Planar Chromatogr. 28, 157-161 (2015). HPTLC of neutral lipids (1) and polar lipids (2) on silica gel with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 for (1) and with chloroform methanol - water 65:25:4 for (2). Detection with phosphomolybdic acid for (1) and cupric sulfate - phosphoric acid for (2). Quantitative determination by absorbance measurement at 610 nm for (1) and 370 nm for (2). The effects of both, high temperature and S. mansoni infection, had individual and combined deleterious effects on lipid metabolism of B. glabrata snails.

Food Chem. 239, 865-871 (2018). NIR spectroscopy for the analysis of oleic, palmitic, linoleic, linolenic, omega-3 and omega-6 acids, unsaturated and saturated fatty acids, and triacylglycerides was compared with HPTLC and GC methods. HPTLC of polar (1) and neutral (2) lipids in salmon oil on silica gel with methyl acetate – isopropanol – chloroform – methanol – potassium chloride (0.25 % solution) 25:25:25:10:9 for (1) and with n-hexane – diethyl ether – glacial acetic acid 35:15:1. Detection by dipping into primulin reagent (10 mg primuline, 40 mL water, 160 mL acetone) for 2 s. The NIR method permits the simultaneous quantification of several fatty acids, but the accuracy and robustness of the method remain at the screening level for palmitic and linolenic acids and monounsaterated fatty acids. The method is not sensitive enough to detect low concentrations of fatty acids, therefore direct measurements on salmon tissue samples were not considered. The NIR method requires a previous extraction of the lipids, similar to the chromatographic techniques. Still the NIR method is a fast, direct and not destructive technique for salmon oil sample screening purposes.

J. Chromatogr. 299, 460-464 (1984). Two-dimensional TLC of various triglycerides on Multi-K plates consisting of a strip of octadecyl-bonded silica along one side of a commercial silica plate. Development along the reversed-phase band with acetone - acetonitrile 4:1 (3 times) followed by dipping the plate into a 10 % silver nitrate solution in water - ethanol 1:1. Development in the second direction with toluene - ethanol 1:1. Detection with 5 % ammonium hydrogen sulfate and 7.5 % molybdophosphoric acid, both in water - ethanol 1:1. Method suitable for semi-quantitative analysis of fat samples.

J. Biochem. (Tokyo) 98, 265-268 (1985). New detection method; detection limit 1 mg; mass spectroscopy after elution.