J. Chromatogr. B 691, 321-329 (1997). HPTLC on silica by AMD with a 25-step gradient, based on methanol, ether and hexane. Detection by spraying with a solution of MnCl2-H2-SO4- at 130°C, or with a solution of CuSO4-H3PO4 at 140°C. Quantification of 5 components by densitometry at 550 nm.

J. Liq. Chrom. & Rel. Technol. 22, 1539-1545 (1999). HPTLC of neutral lipids on silica gel after pre-cleaning of the plates by development to the top with dichloromethane - methanol 1:1 with petroleum ether - ether - acetic acid 80:20:1. Detection after drying by spraying with 5% phosphomolybdic acid in ethanol, and heating in a 110°C oven for 15 min. Densitometry at 700 nm.

coli by TLC with thionine as spot-test reagent. Recognition of cardiolipin, phosphatidylglycerol, phosphatidylethanolamine, and N-acyl phosphatidylethanolamine. J. Planar Chromatogr. 15, 23-27 (2002). One-and two-dimensional TLC of phospholipids (phosphatidylethanolamine, phosphatidylglycerol, cardiolipin, and N-acyl phosphatidylethanolamine) on silica gel with 1-propanol - chloroform - ethyl acetate - methanol - water 50:50:50:21:18 for one-dimensional separation. Visualization by treatment for 20 min with aqueous thionine and differential staining with 0.05 M sulfurous acid. Additional phospholipid-profile information was obtained by separation on aluminium oxide with chloroform - methanol - 2-propanol - water 100:25:2:2 and staining with biebrich scarlet. 2D-TLC with 1-propanol - chloroform - ethyl acetate - methanol - water 50:50:50:21:18 in the first direction, treatment (in-situ) with 1% HCl and development with hexane - diethyl ether 5:1 in the second direction. Visualization with Schiff's leukofuchsin reagent.

CBS 81, 2-5 (1998). The performance and reliability of result of both procedures is comparable - HPTLC being slightly better than HPLC. For the assay of phospholipids in pure substances, pharmaceutical products and lecithin HPTLC is more cost efficient than HPLC by a factor of 2.5.

J. Liq. Chromatogr. Relat. Technol. 31, 1871-1880 (2008). HPTLC for the determination of neutral lipid profils using a standard mixture (containing cholesterol, oleic acid, triolein, methyl oleate, cholesteryl oleate) on silica gel (plates with 19 scored lanes and a preadsorbent application area, prewashed by development with dichloromethane - methanol 1:1) with petroleum ether - diethyl ether - acetic acid 80:20:1 in a saturated twin-trough chamber. Detection by spraying with 5 % ethanolic phosphomolybdic acid and heating for 10 min at 115 °C. Quantitative determination by absorbance measurement at 610 nm.

J. Planar Chromatogr. 26, 202-208 (2013). HPTLC of egg yolk lipid fractions on silica gel with hexane - diethyl ether - formic acid 40:10:1. Detection by srpaying with 10 % copper(II) sulfate in 8 % phosphoric acid. Quantitation by scanning with a flatbed scanner and a gel analysis software. Intermediate/interday/intra-day precision was below 10 % CV (n=6).

Marine Drugs 11 (2), 363-376 (2013). Lipid A fraction was isolated from Escherichia coli strain W3110 and from its plasmid-transformed derivatives HW000, HW001 and HW002 through an adapted three-step version of the Bligh and Dyer’s method. To control the transformation, TLC of the lipid extract, dissolved in chloroform – methanol 4:1, on silica gel with chloroform – methanol – water – ammonia 40:25:4:2. Detection by spraying with 10 % sulfuric acid in ethanol and heating at 175 °C; lipids were visualized as dark zones. Lipid A (hRf 40) was well separated from monophosphoryl lipid A (MPLA) (hRf 60) and from pentaacylated MPLA (hRf 50)._x000D_

Separations 5, 1-11 (2018). HPTLC of lipid content in yeast (clonal prion-infected and prion-free cells) on silica gel with 1) petroleum ether – diethyl ether – glacial acetic acid 80:20:1 for free sterols, free fatty acids, and triacylglycerols, 2) hexane – petroleum ether – diethyl ether – glacial acetic acid 50:20:5:1 for steryl esters, methyl esters, and squalene and 3) chloroform – diethyl ether – acetic acid 130:50:9 for phospholipids (phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinositol). Detection of neutral lipids by spraying with 5 % phosphomolybdic acid in ethanol, followed by heating at 120 °C for 30 min. Detection of phospholipids by spraying with 10 % cupric sulfate in 8 % phosphoric acid, followed by heating at 140 °C for 30 min. Evaluation at 370 nm (deuterium lamp) for phospholipids and at 610 nm (halogen-tungsten lamp) for neutral lipids. The hRf values for neutral lipids were 10 for cholesterol, 33 for oleic acid and 51 for triolein as well as 41 for methyl oleate, 56 for cholesteryl oleate and 77 for squalene. The hRf values of phospholipids were 21 for phosphatidylinositol, 27 for phosphatidylethanolamine and 48 for phosphatidylcholine. HPTLC demonstrated to be a powerful tool for revealing subtle changes in the physiology of yeast.