Prostaglandins Leukotr Essent Fatty Acids 52, 167-171 (1995). TLC of lipids on silica with chloroform - methanol - acetone - acetic acid - water 10:3:3:2:1. Visualization by exposure to iodine vapor. After elution extraction counting for radioactivity in a liquid scintillation system.

Phytochemistry 45, 1351-1357 (1997). Study of the hydroxylation of fatty acids by various enzymes by TLC on silica with chloroform - methanol 4:1 or ether - petrol ether - formic acid 70:30:1. Determination of 12-hydroxy-, 9,10-dihydroxy- and epoxy-lauric acid by autoradiography.

J. Agric. Food Chem. 46, 2634-2637 (1998). TLC of lipids (neutral lipids, phosphatidylcholine, cholesterol oxidation products) on silica gel with hexane - ether - formic acid 80:20:1. Detection by spraying with 10% sulfuric acid in methanol and heating at 130°C.

J. Sliwiok (ed.): Acta Chromatographica 10, 183-189 (2000). HPTLC of triacylglycerols, free sterols, cholesteryl ester, phosphatidylethanolamine, phosphatidylcholine, and free fatty acids in the digestive gland-gonad complex of Cerithidia californica snails infected with Euhaplorchis californiensis, Cloacitrema michiganensis, and Mesostephanus appendiculatus on silica gel with either petroleum ether - diethyl ether - acetic acid 80:20:1 or (esp. for cholesterol esters) n-hexane - petroleum ether - diethyl ether - glacial acetic acid 50:20:5:1 or (esp. for phospholipids) with chloroform - methanol - water 65:25:4. Quantification of neutral lipids at 700 nm after derivatization with phosphomolybdic acid and of phospholipids at 370 nm after derivatization with cupric sulfate reagent.

An in vitro study. J. Planar Chromatogr. 15, 396-403 (2002). One- and two-dimensional TLC of cardiolipin, monolysocardiolipin, phosphatidyl ethanolamine, and phosphatidyl choline on silica gel, sequentially washed with chloroform - methanol 2:1 and acetone, in a twin-trough chamber after saturation for a minimum of 1 h. 2D-TLC with 1-propanol - chloroform - ethyl acetate - methanol - water 50:50:50:21:18 or 0.05 mL of 14 m NH3 added to 20 mL of the a.m. mobile phase in the first direction and hexane - ether 4:1 in the second dimension, after a hydrolysis step (1% HCl), followed by reaction with Schiff leucofuchsin reagent. Differential staining of the different phospholipids in the one-dimensional chromatogram was performed as described by F. M. Helmy and M. H. Hack, J. Chromatogr. 374, (1986) 61-72. Densitometry at 600 or 560 nm.

J. Liq. Chromatogr. Relat. Technol. 28, 2597-2606 (2005). HPTLC of free sterol, and free fatty acids (cholesterol, triacylglycerol and methyl esters) on silica gel (prewashed with dichloromethane - methanol 1:1) with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 in a twin-trough chamber saturated for 15 min. Determination of steryl esters with n-hexane - petroleum ether - diethyl ether - glacial acetic acid 50:25:5:1. Detection by spraying with 5 % ethanolic phosphomolybdic acid solution and heating for 10 min at 115 °C. Determination of polar lipids (cholesterol, phosphatidyl ethanolamine, phosphatidylcholine, lysophosphatidylcholine) with chloroform - methanol - water 65:25:4. Detection by spraying with a 10 % cupric sulfate solution and heating at 140 °C for 10 min. Quantitation by densitometry at 610 nm (for neutral lipids) and 370 nm (for polar lipids).