J. Planar Chromatogr. 34, 411-418 (2021). HPTLC of sphingomyelin in erythrocyte membranes of patients on silica gel with chloroform - methanol - acetic acid - water 60:50:1:4. Detection by exposure to iodine vapor for 30 min. Quantitative determination by densitometric measurement of the intensity of individual zones and the red, green and blue values of the component colors. The hRF value for sphingomyelin was 86. Linearity was between 0.25 and 10 μg/zone. LOD and LOQ were 140 and 410 ng/zone, respectively. Intermediate precisions were below 2 % (n=3). Recovery was between 85 and 97 %.

J. Food Compos. Anal. 99, 103869 (2021). HPTLC of phosphatidylcholine (1), phosphatidylethanolamine (2), phosphatidylserine (3), lysophosphatidylcholine (4), and cardiolipin (5) in edible insects (crickets, migratory locusts, and silkworms) on silica gel with chloroform - methanol - 28 % ammonia 13:7:1. Detection by exposure to iodine. The hRF values for (1) to (5) were 24, 51, 13, 15 and 70, respectively. 

Chemosphere. 90, 2157-2171 (2013). HPTLC of glycerophospholipids in liver and brain of male Atlantic cod (Gadus morhua) on silica gel (washed with methyl acetate - isopropanol - chloroform - methanol - 0.25 % KCl 25:25:25:10:9 and activated at 120 ºC for 30 min) with hexane - diethylether - acetic acid 40:10:1. Detection by spraying with 0.1 % dichlorofluorescin in 98 % methanol and 0.001 % 3,5-di-tert-4butylhydroxytoluene (BHT). Lipid classes were futher analyzed by gas chromatography with a flame ionization detector. 

Anal. Bioanal. Chem. 411, 1181-1192 (2019). HPTLC of cholesterol in fresh egg yolk on silica gel with n-hexane - ethyl acetate - acetic acid 15:9:1. Detection by dipping into p-anisaldehyde sulfuric acid reagent solution for 1 s (1 mL p-anisaldehyde with a refrigerated solution of glacial acetic acid/concentrated sulfuric acid in methanol in a ratio of 1:2:100). Quantitative determination by absorbance measurement at 525 nm. Evaluation under daylight and UV light. The method allowed to study cholesterol-lowering properties of 12 lactic acid bacteria (LAB) in the absence or presence of 0.3 % bile salts. The hRF value for cholesterol was 71. Linearity was between 1 and 10 μg. Intermediate precision was below 7 % (n=3). The LOD and LOQ for cholesterol were 0.2 and 0.8 μg, respectively. Recovery was between 96.6 and 100.1 %. 

Anal. Bioanal. Chem. 412, 2237-2249 (2020). HPTLC of sphingomyelin (1), phosphatidylcholine (2), and triacylglycerol (3) fractions from preadipocytes and adipocytes on silica gel with chloroform - ethanol - water - triethylamine 30:35:7:35 for (1) and (2) and hexane - diethyl ether - acetic acid 80:20:1 for (3). Detection by dipping into primuline (dissolved in acetone - water 4:1) and under UV light at 366 nm. Lipid fractions were further analyzed by electrospray ionization-ion trap mass spectrometry.

J. Liq. Chromatogr. Relat. Technol. 44, 148-170 (2021). Comprehensive review of the contribution of HPTLC to the analysis of lipids in the last ten years. Advances in detection, identification and determination of main classes of lipids and their subclasses separated by HPTLC in different matrices were discussed. The review described techniques for scanning densitometry of lipids through direct detection and on-plate staining using inorganic and organic agents, immunostaining of glycosphingolipids and labeled lipids. Techniques coupling HPTLC with mass spectrometry were also discussed, including extraction-based interfaces, ionization of lipids, on-plate matrix assisted laser desorption and ionization, and its application in the analysis of sphingolipids, neutral lipids and fatty acids and phospholipids.

Heliyon 6(8), e04654 (2020). HPTLC of ethanolic extracts of three algae (100µg/band) on silica gel, along with carotenoid standards (10µg/band), developed with toluene – acetone 7:3. Detection under white light. Carotenoids appeared orange or yellow, chlorophylls green, pheophytins dark khaki. Carotenoid patterns of the algae were very different depending on the family: red alga Eucheuma denticulatum (Solieriaceae) contained mainly zeaxanthin and lutein (hRF 44) and β-carotene (hRF 88), but also β-cryptoxanthin (hRF 69-71) and fucoxanthin (hRF 39); brown alga Sargassum polycystum (Sargassaceae) contained mainly fucoxanthin, and also cryptoxanthin; green alga Caulerpa lentillifera (Caulerpaceae) contained mainly zeaxanthin, but also astaxanthin (hRF 61) and canthaxanthin (hRF 77) in smaller amounts. Separately, HPLC-MS was used to confirm and quantify these compounds, which was necessary for carotenoids with similar hRF values: zeaxanthin and lutein (hRF 44), and β-carotene and lycopene (hRF 88).

Rapid Commun. Mass Spectrom. 33, 60-65 (2019). TLC of oxidation products of 1‐palmitoyl‐2‐oleoyl‐sn‐phosphatidylcholine (POPC) and 1‐palmitoyl‐2‐linoleoyl‐sn‐phosphatidylcholine (PLPC) on silica gel (NP) and RP-18 with chloroform - ethanol - water - triethylamine 20:35:7:35 for NP-TLC and methanol - chloroform - water 10:1:1 for RP-TLC. Detection for NP-TLC under UV 366 nm after spraying with primuline, and for RP-TLC under white light after spraying with molybdatophosphoric acid solution, followed by heating at 130 ºC. Lipids zones from both plates were eluted with methanol and further analyzed by electrospray ionization mass spectrometry.