J. Agric. Food Chem. 46, 2634-2637 (1998). TLC of lipids (neutral lipids, phosphatidylcholine, cholesterol oxidation products) on silica gel with hexane - ether - formic acid 80:20:1. Detection by spraying with 10% sulfuric acid in methanol and heating at 130°C.

J. Sliwiok (ed.): Acta Chromatographica 10, 183-189 (2000). HPTLC of triacylglycerols, free sterols, cholesteryl ester, phosphatidylethanolamine, phosphatidylcholine, and free fatty acids in the digestive gland-gonad complex of Cerithidia californica snails infected with Euhaplorchis californiensis, Cloacitrema michiganensis, and Mesostephanus appendiculatus on silica gel with either petroleum ether - diethyl ether - acetic acid 80:20:1 or (esp. for cholesterol esters) n-hexane - petroleum ether - diethyl ether - glacial acetic acid 50:20:5:1 or (esp. for phospholipids) with chloroform - methanol - water 65:25:4. Quantification of neutral lipids at 700 nm after derivatization with phosphomolybdic acid and of phospholipids at 370 nm after derivatization with cupric sulfate reagent.

An in vitro study. J. Planar Chromatogr. 15, 396-403 (2002). One- and two-dimensional TLC of cardiolipin, monolysocardiolipin, phosphatidyl ethanolamine, and phosphatidyl choline on silica gel, sequentially washed with chloroform - methanol 2:1 and acetone, in a twin-trough chamber after saturation for a minimum of 1 h. 2D-TLC with 1-propanol - chloroform - ethyl acetate - methanol - water 50:50:50:21:18 or 0.05 mL of 14 m NH3 added to 20 mL of the a.m. mobile phase in the first direction and hexane - ether 4:1 in the second dimension, after a hydrolysis step (1% HCl), followed by reaction with Schiff leucofuchsin reagent. Differential staining of the different phospholipids in the one-dimensional chromatogram was performed as described by F. M. Helmy and M. H. Hack, J. Chromatogr. 374, (1986) 61-72. Densitometry at 600 or 560 nm.

J. Liq. Chromatogr. Relat. Technol. 28, 2597-2606 (2005). HPTLC of free sterol, and free fatty acids (cholesterol, triacylglycerol and methyl esters) on silica gel (prewashed with dichloromethane - methanol 1:1) with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 in a twin-trough chamber saturated for 15 min. Determination of steryl esters with n-hexane - petroleum ether - diethyl ether - glacial acetic acid 50:25:5:1. Detection by spraying with 5 % ethanolic phosphomolybdic acid solution and heating for 10 min at 115 °C. Determination of polar lipids (cholesterol, phosphatidyl ethanolamine, phosphatidylcholine, lysophosphatidylcholine) with chloroform - methanol - water 65:25:4. Detection by spraying with a 10 % cupric sulfate solution and heating at 140 °C for 10 min. Quantitation by densitometry at 610 nm (for neutral lipids) and 370 nm (for polar lipids).

Thin Layer Chromatographic and densitometric analysis. J. Planar Chromatogr. 20, 209-215 (2007). TLC of phospholipids (with cardiolipin, phosphatidyl ethanolamine plasmologen and phosphatidyl cholin plasmologen as standards) on silica gel, prewashed with chloroform - methanol 2:1 and acetone, using one-dimensional TLC with 1-propanol - chloroform - ethyl acetate - methanol - water 50:50:50:21:18 and two-dimensional TLC with 1-propanol - chloroform - ethyl acetate - methanol - water 50:50:50:21:18 in the first direction and hexane - diethyl ether 1:1 in the second direction after hydrolysis with 1 % hydrochloric acid to reveal alkenylphospholipids. Detection by staining with thionine reagent resp. with leucofuchsin reagent. Densitometric scanning at 600 nm (for thionine) and at 560 nm (for leucofuchsin).

J. Liq. Chromatogr. Relat. Technol. 33, 1005-1012 (2010). HPTLC of lipids (free sterols, free fatty acids, triacylglycerols, methyl esters, and steryl esters) on silica gel (plates with concentration zone) with petroleum ether - diethylether - glacial acetic acid 80:20:1. Detection by spraying with 5 % ethanolic phosphomolybdic acid reagent and heating for 10 min at 110 °C. Quantitative densitometric analysis was performed at 610 nm.

J. Sep. Sci. 37, 2062-2068 (2014). HPTLC of (1) phospholipids phosphatidylcholine, (2) phosphatidylethanolamine, (3) phosphatidylinositol, (4) phosphatidylserine and (5) sphingomyelin in Phyrrhocoris apterus on silica gel with chloroform - methanol - acetic acid - water 60:35:2:1 over a path of 9 cm, and chloroform - methanol - acetic acid - water 25:15:4:2 over a path of 6 cm. Detection by exposure to iodine vapor, followed by detection at UV 205 nm. The hRF values of (1) to (5) were 91, 68, 82, 88 and 94, respectively. The spots were subsequently scrapped from the plates. Quantification by conversion into inorganic phosphate (as phosphomolybdate complex) followed by absorbance measuremet at 820 nm.

J. Agric. Food Chem. 64, 8838-8847 (2016). HPTLC of free fatty acids produced after treatment of vegetable oils with crude lipase extracts from germinated seeds of Adansonia suarezensis, Adansonia grandidieri, Moringa oleifera, Moringa drouhardii, Jatropha mahafalensis, and Jatropha curcas seeds on silica gel with hexane – diethyl ether – acetic acid 70:30:1. The hRF value of free fatty acid was 43.