Phytochemistry 31, 1961-1967 (1992). Separation of different lipid classes by two-dimensional TLC on silica with 1) chloroform – methanol – water 65:25:4 and 2) chloroform – acetone – methanol – acetic acid 10:4:2:1. Detection by spraying with 10% primuline in aqueous 80% acetone and under UV.

Phytochemistry 34, 1323-1333 (1993). 2D-TLC of more than 100 species representing all the 16 orders of brown algae (Phaeophyceae) on silica with 1) chloroform - methanol - water 65:25:4 and 2) chloroform - methanol - isopropylamine - conc. NH3 130:70:1-10. Detection under UV 366 nm after spraying with dichlorofluorescein (0.05% in ethanol). For the detection of betaine lipids the plate was sprayed with Dragendorff's reagent.

J. Liquid Chromatogr. 18, 527-535 (1995). HPTLC of cholesterol esters, triacylglycerols, free fatty acids, and cholesterol on silica with petrol ether (37.5 - 52 °) - ether - acetic acid 80:20:2, and hexane - petrol ether - ethyl ether - acetic acid 50:20:2:1. Detection by spraying with 5% phosphomolybdic acid in ethanol and heating at 110-120 °C for 5-10 min. Quantification by densitometry at 700 nm.

J. Planar Chromatogr. 10, 308-310 (1997). TLC of diacylglycerols, cholesterol, free fatty acids and triacylglycerols on silica with hexane - ether - acetic acid 80:20:1. Detection by spraying with three different reagents: I. 3% cupric acetate in 6% aqueous phosphoric acid solution, followed by heating at 100°C for 10 min; II. 35% aqueous sulfuric acid solution, followed by heating at 140°C for 10 min; III. procedure I. followed by procedure II. Densitometry at 430 nm (absorbance). Also published in Proc. 9th Internat. Symp. Instr. Chromatogr., Interlaken, April 9.-11., 331-335 (1997).

J. Lipid Res. 38, 1482-1488 (1997). TLC of phospholipids and neutral lipids on EDTA-impregnated silica gel and after pre-concentration with chloroform - methanol - water 60:40:10 with five step-wise developments: i) chloroform - methanol - water 65:40:5 to 2 cm, ii) ethyl acetate - 2-propanol - ethanol - chloroform - methanol - 0.25% KCl 35:5:20:22:15:9 to 5 cm, iii) toluene -diethyl ether - ethanol 60:40:3 to 7.5 cm, iv) n-heptane - diethyl ether 94:8 to 10.5 cm, v) pure n-heptane to 12.5 cm. Charring by dipping in a solution of 10% cupric sulfate in 8% phosphoric acid for 10 s and heating at 200°C for 2 min. Quantitative determination with an image analyzer in transmission mode.

J. Lipid Res. 41, 142-147 (2000). 2-D TLC of lysophospholipids (lysophosphatidic acid, lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylglycerol, lysophosphatidylinositol, lysophosphatidylserine, sphingosylphosphorylcholine, sphingosine-1-phosphate) on silica gel in the first direction with 1) chloroform - methanol - formic acid - water 60:30:7:3 and in the second direction with 2) chloroform - methanol - 28% NH3 - water 25:29:4:1. To separate lysophospholipids from neutral lipids on the same plate, a third run was performed with ether in the opposite direction to the second run.

J. Planar Chromatogr. 16, 405-407 (2003). HPTLC of neutral lipids (e.g. cholesteryl oleate, methyl oleate, triolein, oleic acid, and cholesterol) on silica gel (with prescored lanes), after prewashing with dichloromethane - methanol 1:1, for the methyl ester, triacylglycerol, free fatty acids, and free sterol content with petroleum ether - ether - acetic acid 80:20:1 and for cholesteryl esters with hexane - petroleum ether - ether - acetic acid 50:25:5:1. Development of plates in a presaturated chamber at 22°C and a humidity of 50%. Visualization by spraying with a solution of 5 g phosphomolybdic acid in 100 mL absolute ethanol and heating for 10 min at 115°C. TLC of polar lipids (cholesterol, phosphatidylethanolamine, phosphatidylcholine, and lysophosphatidylcholine) on silica gel with chloroform - methanol - water 65:25:4. Visualization by spraying with a 10% cupric sulfate solution (prepared by dissolving 100 g CuSO4 and 100 mL phosphoric acid in water and making up to 1 L), followed by heating for 10 min at 140°C. Quantitative densitometry at 610 nm for neutral lipids and at 370 nm for polar lipids.

J. Liq. Chromatogr. Relat. Technol. 28, 2571-2580 (2005). TLC of lipids, triacylglycerol, diacylglycerol, and sphingosine on silica gel with hexane - diethyl ether - formic acid 40:10:1 for neutral lipids, and with chloroform - methanol - water 65:25:4 for glycolipids. Phospholipids were separated by two dimensional development with chloroform - methanol - 28 % ammonium hydroxide 65:25:4 in the first direction, followed by drying and development with chloroform - acetone - methanol - acetic acid - water 30:40:10:10:1 in the second direction. Detection of neutral lipids by spraying with charring or phosphomolybdic acid reagent; detection of glycolipids by spraying with orcinol reagent (orcinol in 70 % sulfuric acid), and of phospholipids by spraying with molybdenum blue reagent for phosphate groups or ninhydrin reagent for phospholipids containing free amino groups.