Chinese Anal. Chem. (Fenxi Huaxue) 26, 81-84 (1998). TLC on silica, development twice with 1) chloroform - methanol - acetic acid - acetone - water 35:25:4:14:2, 2) hexane - ether 4:1. Detection by spraying with 10% phosphomolybdic acid in ethanol and heating at 120°C for 10 min, and with Dittmer reagent. Quantitation of phosphatidylcholine (pc), cholesterol (ch), and glycerolipid (gly) by densitometry at 650 nm. Detection limit <0.2 mg/spot. Recovery 104% (n=5) for pc.

J. Liq. Chrom. & Rel. Technol. 19, 3125-3134 (1996). TLC or HPTLC of polar and non-polar lipids on silica gel with n-hexane - ether - acetic acid 80:20:1; for non-polar lipids, and chloroform - methanol - water 65:25:4; for polar lipids. Visualization by exposure to iodine vapor. Quantitation by densitometry using reflection mode at 365 nm. New simple TLC method.

I. Separation of human brain lipids. J. Liqu. Chromatogr. 25, 1255-1269 (2002). HPTLC of lipids (glycerophospholipids, sphingomyelin, glycosphingolipids, cholesterol) on silica gel with chloroform - methanol - 0.2% aqueous calcium chloride solution 15:8:1; 90:12:1. Detection by spraying with orcinol reagent and Dittmer's reagent.

J. Liq. Chrom. Rel. Technol. 27, 2039-2045 (2004). HPTLC of cholesteryl oleate, methyl oleate, triolein, oleic acid, and cholesterol on pre-washed silica gel with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 in a twin-trough chamber with saturation. Detection with phosphomolybdic acid reagent. Quantitative determination at 610 nm.

Anal. Bioanal. Chem. 389, 827–834 (2007). A total extract of hen egg yolk is used as phospolipid mixture to demonstrate the capabilities. TLC of phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, phosphatidylethanolamine, lysophosphatidylethanolamine and phosphatidylinositol on silica gel with chloroform - ethanol - water - triethylamine 5:5:1:5. Detection under UV 366 nm after spraying with primuline (Direct Yellow 59) reagent. Direct coupling MALDI-TOF MS and TLC can be easily implemented on commercially available MALDI-TOF devices. Roughly clean spectra without major contributions from fragmentation products and matrix peaks were obtained. This approach was sensitive enough to detect the presence of phospholipids at levels of less than 1 % of the total extract.

J. Liq. Chromatogr. Relat. Technol. 36, 2489-2496 (2013). HPTLC of neutral and polar lipids in Biomphalaria glabrata snails subjected to either Echinostoma caproni and Schistosoma mansoni miracidia coexposure, on silica gel with petroleum ether - diethyl eter 4:1 + 1 drop glacial acetic acid for neutral lipids and chloroform - methanol - water 65:25:4 for phospholipids. Detection by spraying with 5 % ethanolic phosphomolybdic acid for neutral lipids and 10 % cupric sulfate in 8% phosphoric acid for polar lipids. Quantitative determination by absorbance measurement at 610 nm and 370 nm for neutral and polar lipids, respectively.

PLoS ONE 10(10), e0140782 (2015). HPTLC of lipids, extracted from several cancerous cell lines by an adapted Bligh and Dyer’s method, on silica gel with chloroform – methanol 4:1. Detection under UV after spraying with thioflavine S (10 μg/mL in acetone 80 %), glycosylated lipids were hued in red-rose by spraying with orcinol 0.5 % in sulfuric acid 0.5 M followed by heating; some of those lipids were absent from the elisidepsin-resistant strains. These lipids were purified by scraping off the plate and by extracting with chloroform – methanol 2:1, then they were applied on a nitrocellulose membrane for an overlay assay for binding with an elisidepsin-biotin derivative (dot-blotting). One lipid was positive and was identified by NMR as probably a glucosylceramide.

Food Res. Int. 111, 361-370 (2018). HPTLC of triacylglycerols in unfermented and fermented cocoa beans on silica gel with hexane – diethyl ether – glacial acetic acid 80:20:1 and then with chloroform – methanol – glacial acetic acid 97:3:1. Detection by spraying with cerium molybdate reagent. Direct comparison of fermented and unfermented beans revealed the absence of cacaoic acid at hRf 55._x000D_