JAOAC 84, 1277-1282 (2001). HPTLC of 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG), cholesterol, alkylphosphocholines, and analogues and their related compounds (main degradation products) on silica gel with chloroform (for cholesterol), chloroform - methanol - 25% NH3 (for DPPG-sodium) and chloroform - methanol - sodium acetate 1 mol/l in 25% NH3 7:4:1 for miltefosine and perifosine. For AMD, 2 new gradient systems were developed. Visualization by dipping into a copper (II) sulfate solution (10 g copper sulfate dissolved in 100 ml 8% phosphoric acid) for a few seconds and heating of the plates to 180°C for 20 min. Quantification by densitometry at 500 nm.

J. Planar Chromatogr. 18, 141-146 (2005). TLC of fatty acids (ethanoic, propanoic, butanoic, pentanoic, hexanoic, heptanoic, and octanoic acid) on silica gel with hexane - acetone 4:1 or acetone - water - chloroform - ethanol - aqueous ammonia 30:1:3:5:11 with chamber saturation for 30 min. Only bromocresol green, bromophenol blue, potassium permanganate, and methyl red can be used for the detection. Among the new visualizing agents dipping of the plates in an aqueous solution of alkaline blue enables the detection of the free fatty acids from propanoic to octanoic. Dipping generated better contrast regarding the spots than spraying.

J. Planar Chromatogr. 22, 35-42 (2009). HPTLC of phospholipids (lyso-phosphatidylcholine, sphingomyelin, phosphatidylcholin, lyso-phosphatidylethanolamine, phosphatidylinositol, phosphatidylethanolamine, phosphatidylserine, phosphatidic acid, and triacylglycerol) on silica gel with chloroform - ethanol - water - triethylamine 5:5:1:5. Detection by spraying with a solution of primulin (Direct Yellow 59). Evaluation under UV 366 nm. Detection by MALDI-TOF MS directly on the plates.

J. Planar Chromatogr. 26, 486-490 (2013). HPTLC of tallow in cow ghee on silica gel with n-hexane - diethyl ether 2:3 + 1 drop glacial acetic acid for unsaponifiable fraction (1) and n-hexane - diethyl ether 13:7 + 1 drop glacial acetic acid for saponifiable fraction (2). Detection by spraying with 10% methanolic sulfuric acid reagent followed by heating at 110 ºC for 5-10 min. Zones at hRf values of 9 and 20 were identified as tallow concentration increased.

J. Liq. Chromatogr. Relat. Technol. 39, 322-324 (2016). HPTLC of neutral (1) and polar lipids (2) in Biomphalaria glabrata snails on silica gel with petroleum ether – diethyl ether – glacial acetic acid 80:20:1 for (1) and chloroform – methanol – water 130:50:9 for (2). Detection of neutral lipids by spraying with 5 % phosphomolybdic acid in absolute ethanol and polar lipids using 10 % copper (II) sulfate in 8 % phosphoric acid.

Biochem. Biophys. Res. Commun. 500, 103-109 (2018). 2D-TLC of glycerolipid classes in Arabidopsis thaliana seedlings on silica gel with chloroform – methanol – ammonia 15:10:1 in the first dimension and chloroform – methanol – acetic acid – water 170:20:15:3 in the second dimension. Detection by spraying with 0.01 % primuline in 80 % acetone. Lipid zones were scraped off from the TLC plate and separated lipids were analyzed by gas chromatography.

Lipids 17, 477-482 (1982). Preparative TLC of microbially produced metabolites of lithocholic acid and lithocholic acid-3 alpha-sulfate on silica with dichloromethane-methanol 95:5. Detection by spraying with anisaldehyde, followed by heating. The following metabolites were isolated: isolithocholic acid, 5 beta-cholanic acid, 3-keto-lithocholic acid, 5 alpha -cholestone and delta 3-cholenic acid.

Talanta 31, 489-495 (1985). TLC of cholesterol and lipid oxidation products on silica with several solvent systems. Detection by spraying with 1:1 mixture of conc. sulfuric acid and saturated aq. ceric ammonium sulfate solution and heating at 150 °C for 10 minutes.