Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      99 022
      High Performance Thin Layer Chromatographic analysis of neutral lipids in the feces of BALB/c mice infected with Echinostoma caproni
      S. R. BANDSTRA, K. E. MURRAY, B. FRIED, J. SHERMA* (*Department of Chemistry, Lafayette College, Easton, PA 18042, USA; shermaj@lafayette.edu)

      J. Liq. Chromatogr. & Relat. Technol. 30, 1437-1445 (2007). HPTLC of steryl esters, methyl esters, triacylglycerols, FFA, and free sterols on silica gel (prewashed by development with dichloromethane -methanol 1:1) with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 in a saturated twin-trough chamber. Detection by spraying with 5 % ethanolic phosphomolybdic acid followed by heating at 150 °C for 110 min. Quantitative determination by absorbance measurement at 610 nm.

      Classification: 11c
      102 021
      Quantitative silver ion thin layer chromatography of triacylglycerols from sunflower oils differing in the level of linoleic acid
      Ilko MAREKOV*, R. TARANDJIISKA, S. MOMCHILOVA, B. NIKOLOVA-DAMYANOVA (*Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria)

      J. Liq. Chromatogr. Relat. Technol. 31, 1959-1968 (2008). Quantitative Ag-TLC of eight samples of sunflower oil with different linoleic acid content on silica gel (impregnated by dipping into a 0.5 % methanolic solution of silver nitrate) with petroleum ether - acetone 25:1, and petroleum ether - acetone - ethyl acetate 100:5:2, and 50:3:2. Detection by consecutive treatment with bromine and sulfurylchloride vapors (30 min each) followed by heating at 180-200 °C. Quantitative determination by absorbance measurement at 450 nm. Evaluation of authenticity and possible adulteration of edible oils.

      Classification: 11c
      108 038
      Characterization and quantification of the polar lipids in the lizard Uta stansburiana by HPTLC-densitometry
      J. COUNIHAN, P. ZANI, B. FRIED, J. SHERMA* (*Department of Biology, Lafayette College, Easton, PA 18042, USA, friedb@lafayette.edu)

      J. Liq. Chromatogr. Relat. Technol. 32, 1289-1298 (2009). HPTLC of phosphatidylcholine (1), phosphatidylethanolamine (2), sphingomyelin (3), sulfatides (4), and cerebrosides (5) in tissue samples of the lizard Uta stansburiana on silica gel with chloroform - methanol - water 65:25:4. Detection by spraying with 10 % cupric sulfate in 8 % phosphoric acid, followed by heating at 140 ºC for 30 min. Quantitative determination by absorbance measurement at 370 nm. The hRf values of (1)-(5) were 40, 56, 39, 51 and 71, respectively.

      Classification: 11c
      116 038
      Phytoceramide in vertebrate tissues - one step chromatography separation for molecular characterization of ceramide species
      S. DASGUPTA, Jina KONG, E. BIEBERICH* (*Institute of Molecular Medicine and Genetics, Georgia Regents University, Augusta, Georgia, United States of America; ebieberich@gru.edu)

      PLoS ONE 8(11), e80841 (2013). On silicic acid columns, total ceramides, from methanol – chloroform extracts of mouse tissue (brain, heart, liver) and of cell cultures (astrocytes, oligodendroglia) were purified from other lipids (phospholipids, sphingoid bases, glycosphingolipids, retained on the column) and fractioned using chloroform – acetone – acetic acid 24:1:0.01 followed by chloroform – acetone – acetic acid 18:2:0.01. For the monitoring, TLC of fractions (layer not specified) with chloroform – methanol – acetic acid 190:10:1. After derivatization by iodine vaporization or by spraying with copper sulfate reagent (copper(II) sulfate 20 mM - sulfuric acid 5 % - ammonium molybdate 1 %), lipids are detected as orange or brown-black zones, respectively. The aforesaid fractionation allowed the isolation of hydroxyl-fatty-acyl ceramides, phytoceramides, non-hydroxy-fatty-acyl ceramides and cholesterol (identification by comparison to standards and by GC-MS); their hRf values were between 40 and 80. Quantitative determination by densitometric measurement. This is the first report of phytoceramide identified in vertebrate brain, heart and liver.

      Classification: 11c, 13c
      120 053
      Quantification of wax ester content in escolar
      A. MILLER*, G. MORLOCK (*Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit, Veterinärstr. 2, 85764, Oberschleissheim, Germany, andreas.miller@lgl.bayern.de)

      CBS 117, 11-12 (2006). For the rapid control of the fish species escolar (Lepidocybium flavobrunneum) by determination of the indigestible wax esters, HPTLC on silica gel with n-hexane – toluene 7:3 with chamber saturation for 10 min, migration distance 60 mm. Detection by dipping in aqueous Rhodamine B reagent (0.025 %), evaluation under UV 366 nm. Quantitative determination by fluorescence measurement at 366/>400 nm with the Hg lamp. The hRF value of oleyl oleate was 30 and of stearyl stearate 40. The mean recovery rate (104 %) was determined by analyzing salmon samples spiked to contain 20 % wax esters. The wax ester content of an escolar sample was 18-22 % (n=3). The repeatability was <5 % and the mean laboratory precision 3 %.

      Classification: 11c
      55 032
      Estolide triglycerides of trewia nudiflora seed oil
      R. MADRIGAL, C. SMITH

      Lipids 17, 650-655 (1982). For preparative separation silica plates were used with hexane - ether 6:4. For separation of hydrogenated oils into their various components, multiple development (4 or 5x) TLC was carried out with ether - benzene 1:99 followed by ether - benzene 3:97. Analytical TLC components were visualized by charring with sulfuric acid-dichromate or with iodine vapors. reparative TLC: bands were located by spraying with ethanolic dichlorofluorescein, followed by viewing under UV. The components of the seed oil of trewia nudiflora and mallotus phillipinensis were chromatographed and compared.

      Classification: 11c, 29f
      57 066
      Chromatographic methods for determination of phospholipids from rat brain
      A.T. DISCHKELOV

      V. Danube Symposium on Chromatography, Yalta, November 11.-16., 1985. TLC of phospholipids on silica with chloroform - methanol - water 65:25:4, identification of phospholipids using Bartlet's method.

      Classification: 11c
      58 202
      Interaction of some non-ionic tensides with dioleoylphosphatidyl choline, studied by charge-transfer chromatography
      T. CSERHATI, M. SZOGYI, L. GYORFI

      J. Chromatogr. 349, 295-300 (1985). Study on the interaction of the membrane phospholipid, dioleylphosphatidyl choline with 23 nonionic tensides by charge-transfer. Reversed-phase TLC on cellulose with water - ethanol 12:13. Exposure of the chromatogram to iodine vapor. Discussion of the dependence of the lipophilicity of dioleoyl phosphatidyl choline on tenside concentration of eluent.

      Keywords:
      Classification: 11c, 35a