Anal. Bioanal. Chem. 392, 849-860 (2008). HPTLC of cell lipids on silica gel with chloroform - ethanol - water - triethylamine 5:5:1:5. Detection under UV 366 nm after spraying with primuline (Direct Yellow) reagent. Coupling with MALDI-TOF-MS which is traditionally used for proteomics, but is also a useful tool for lipid analysis. Depending on the applied matrix, however, some lipid classes are more sensitively detected than others and this may even lead to suppression effects if complex mixtures are analyzed. Therefore, a previous separation into the individual lipid classes is necessary. Using artificial lipid mixtures or easily available tissue extracts, it has already been shown that lipids can be conveniently analyzed by MALDI-TOF MS directly on the TLC plate. An initial TLC-MALDI-TOF-MS study of the lipid composition of ovine mesenchymal stem cells is presented. Due to the complex composition of these cells, data are also compared to lipids extracted from human erythrocytes. Even very minor lipid classes can be easily detected and with much higher sensitivity than by common staining protocols.

Trends in Chromatography 8, 1-6 (2013). HPTLC of neutral lipids and a neutral lipid standard (consisting of 20 % each of cholesterol, oleic acid, triolein, methyl oleate, and cholesteryl oleate) on silica gel (HLF plates with 19 scored channels of 9 mm width) prewashed with dichloromethane - methanol 1:1 and heated for 30 min at 120 °C, with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 with chamber saturation. Detection by spraying with 5 % ethanolic phosphomolybdic acid reagent and heating at 120 °C for 5 min. Neutral lipids appeared as blue zones on a yellow background. The fractions of free sterols, free fatty acids, triacylglycerols, methyl esters, and steryl esters were identified by comparison with the standard mixture. Quantification by absorption measurement at 610 nm via linear calibration (peak area).

Planta Medica 82(4), 371-376 (2016). TLC was applied to compare acetone – water 4:1 extracts from the mycelia and medium of fungus Trichoderma koningiopsis YIM PH 30002; an ethyl acetate fraction of each extract was separated on silica gel (mobile phase unknown) and visualized by three methods: detection with iodine reagent, sulfuric vanillin reagent, and phosphomolybdic acid in ethanol (8 %, w/v); no difference was found. Both extracts were then combined for the further isolation on columns of koninginins A, B and F, koningiopisins A–H, and trichodermaketone C._x000D_

Biochem. Biophys. Res. Commun. 491, 265-270 (2018). HPTLC of nitrobenzoxadiazole (NBD)-labeled lipids in the reaction mixture of ceramide glucosyltransferase (GlcT-1) on silica gel with chloroform – methanol – water 65:25:4. NBD-labeled lipids were visualized by UV-B illumination. The amount of C6-NBD-glucosylceramide (GlcCer) generated was quantified by TLC image processing. The method was used to study the effects of JAK/STAT3 pathway inhibitors on GlcT-1 activity.

J. High Resol. Chromatogr. 7, 473-476 (1984). HPTLC of phospholipids on silica with chloroform - acetone - methanol - acetic acid - water 3:4:1:1:0.6. Detection by dipping into MnCl2-reagent and heating at 120 °C for 20 minutes, after cooling down dipping into paraffin-hexane 1:2 and drying. In situ fluorometry.

Clin. Chim. Acta 147, 183-90 (1985). TLC of sphingomyelin, phosphatidylcholine, phosphatidylserine and phosphatidylethanolamine on silica with chloroform - methanol - acetic acid - water 100:55:16:6. Detection by staining with Naphtho Blue Black. Quantification by spectrodensitometry.

Acta Veterinaria 34, 73-80 (1986). TLC of polar lipids on silica with chloroform - methanol - water 65:25:4 and of nonpolar lipids on silica with petrol ether - ether - acetic acid 80:20:1. Detection by UV.

Anal. Biochem. 163, 27-35 (1987). TLC of various glycosphingolipids on silica coated with a thin film of poly (iso-Bu methacrylate). Plate placed in a chamber containing metabolically radiolabelled cells, sealed and centrifuged to bring the cells into contact with the surface of the plate. Development of the cell adhesion blocked by a solution of N-acetyl-glycosamine in various solvents. Detection by autoradiography. Detection limit, 8 pmol.