J. Planar Chromatogr. 28, 157-161 (2015). HPTLC of neutral lipids (1) and polar lipids (2) on silica gel with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 for (1) and with chloroform methanol - water 65:25:4 for (2). Detection with phosphomolybdic acid for (1) and cupric sulfate - phosphoric acid for (2). Quantitative determination by absorbance measurement at 610 nm for (1) and 370 nm for (2). The effects of both, high temperature and S. mansoni infection, had individual and combined deleterious effects on lipid metabolism of B. glabrata snails.

Food Chem. 239, 865-871 (2018). NIR spectroscopy for the analysis of oleic, palmitic, linoleic, linolenic, omega-3 and omega-6 acids, unsaturated and saturated fatty acids, and triacylglycerides was compared with HPTLC and GC methods. HPTLC of polar (1) and neutral (2) lipids in salmon oil on silica gel with methyl acetate – isopropanol – chloroform – methanol – potassium chloride (0.25 % solution) 25:25:25:10:9 for (1) and with n-hexane – diethyl ether – glacial acetic acid 35:15:1. Detection by dipping into primulin reagent (10 mg primuline, 40 mL water, 160 mL acetone) for 2 s. The NIR method permits the simultaneous quantification of several fatty acids, but the accuracy and robustness of the method remain at the screening level for palmitic and linolenic acids and monounsaterated fatty acids. The method is not sensitive enough to detect low concentrations of fatty acids, therefore direct measurements on salmon tissue samples were not considered. The NIR method requires a previous extraction of the lipids, similar to the chromatographic techniques. Still the NIR method is a fast, direct and not destructive technique for salmon oil sample screening purposes.

J. Chromatogr. 299, 460-464 (1984). Two-dimensional TLC of various triglycerides on Multi-K plates consisting of a strip of octadecyl-bonded silica along one side of a commercial silica plate. Development along the reversed-phase band with acetone - acetonitrile 4:1 (3 times) followed by dipping the plate into a 10 % silver nitrate solution in water - ethanol 1:1. Development in the second direction with toluene - ethanol 1:1. Detection with 5 % ammonium hydrogen sulfate and 7.5 % molybdophosphoric acid, both in water - ethanol 1:1. Method suitable for semi-quantitative analysis of fat samples.

J. Biochem. (Tokyo) 98, 265-268 (1985). New detection method; detection limit 1 mg; mass spectroscopy after elution.

J. Chromatogr. 374, 61-72 (1986). TLC of phosphatidyl ethanolamine, phosphatidylcholine on silica with cyclohexane - isopropanol - water 30:40:6, and on alumina with chloroform - methanol - water 65:30:4. Various detection methods. Modified method enhancing reliability of glycerophospho and neutral lipid assay.

J. Chromatogr. 363, 462-467 (1986). Conversion of phospholipids to dimethyl esters of phosphatidic acids with the exception of phosphatidylinositol which was converted to the pentaacetyl derivatives. TLC on AgNO3-impregnated silica with mobile phases containing chloroform - methanol. The method was used for the analysis of molecular species of egg lecithin.

H. TRAITLER, A. STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institute for Chromatography, Bad Dürkheim, FRG (1987), 379-387. Anticircular TLC of impurities of Jojoba wax esters and decomposed triglycerides on very thin silica layer with petrol ether - ether 6:4. Detection by dipping in manganese chloride - sulfuric acid reagent and heating at 150 °C for 50 sec. Quantification by densitometry at 546 nm.

J. Chromatogr. 446, 259-266 (1988). Reverse-phase TLC on silanized Kieselguhr G with acetone - acetonitrile - water in different proportions. Densitometry at 450 nm. Investigation of the relationship between partition numbers and the maximum-to-maximum distances of the corresponding peaks. Identification by their partition numbers. Presentation of an example concerning sunflower oil triglycerides.