edulis and gracilis. Planta Med. 58, 544-548 (1992). TLC of flavonoids and phenol C-glycosides on silica with ethyl acetate - formic acid - acetic acid - water 100:11:11:26. Detection with 1% diphenylborylexyethylamine, followed by 5% PEG 4000 in ethanol or with 1% vanillin in ethanol, followed by 10% sulfuric acid in ethanol. Also TLC of flavonol rhamnosides on silica with chloroform - methanol - acetic acid - water 60:32:12:8.

Planta med. 65, 56-59 (1999). Anal. and preparative TLC of dihydroquercetin, resveratrol, and engeletin on silica gel with chloroform - methanol 5:1. Detection under UV or by spraying with cerium sulfate - 10% sulfuric acid 1:99. Also TLC of rhamnose with n-butanol - water - acetic acid 4:2:1.

Food Chem. 114, 1413-1420 (2009). HPTLC of methanolic fractions of stingless bee honey samples and commercial samples from Apis mellifera (European honey bee) on silica gel with a five step development with two different mobile phases: ethyl acetate - formic acid - acetic acid - water 100:11:11:27 and toluene - ethyl acetate - acetic acid 10:9:1. Detection by fluorescence measurement at 400 nm and absorbance measurement at 240 nm, after fluorescence induction at 365 nm with a mercury vapor lamp. Detection of flavonoids by spraying with an aqueous solution of 4 % aluminium sulphate. Flavonoids and coumarins were identified by comparison with commercial standards.

J. Planar Chromatogr. 26, 306-311 (2013). HPTLC of baicalein (1), hispidulin (2), chrysin (3), and oroxylin A (4) in different plant parts of Oroxylum indicum on silica gel with chloroform - methanol 97:3 + 1 drop water + 1 drop formic acid. Quantitation by absorbance measurement at 270 nm. The hRf values for compounds (1) to (4) were 22, 2, 36 and 61, respectively. Linearity was in the range of 500-2500 ng/zone for (1) to (4). LOD and LOQ were 77 and 257 ng/zone for (1), 99 and 330 ng/zone for (2), 69 and 229 ng/zone for (3) and 45 and 151 ng/zone for (4), respectively. Average recoveries were 98.3 % for (1), 95.2 % for (2), 100.2 % for (3) and 98.0 % for (4). Intermediate/interday/intra-day precision was below 5 % (n=5).

J. Liq. Chromatogr. Relat. Technol. 38, 1392-1406 (2015). HPTLC of Cordyceps sinensis and Ganoderma lucidum on silica gel with ethyl acetate - dichloromethane - formic acid - glacial acetic acid - methanol 10:10:1:1:2. Qualitative identification at UV 254 nm and UV 366 nm. Hyphenation of electronspray ionization/mass spectrometry with HPTLC facilitated fast and convenient profiling of the metabolites in the extracts. For the chemometric analysis, raw and column data matrices were constructed using hRF datasets.

J. Ethnopharmacol. 182, 101-109 (2016). HPTLC of quercetin (1) and chlorogenic acid (2) in the leaves of Moringa oleifera on silica gel with toluene – ethyl acetate – formic acid 5:4:1 for (1) and ethyl acetate – dichloromethane – formic acid – acetic acid – water 100:25:10:10:11 for (2). Quantitative determination by absorbance measurement at 375 nm for (1) and 330 nm for (2). The hRF values of (1) and (2) were 63 and 30, respectively.

Planta Medica 82 (17), 1475-1481 (2016). Four new flavone glycosides (acacetin group) isolated from Piper aduncum leaves were submitted to acidic hydrolysis; TLC of the aqueous fractions, purified with ethyl acetate, on silica gel and RP-18 with chloroform – methanol – water 30:20:3. Detection by spraying with sulfuric acid reagent, followed by heating. By comparison to standards (applied in overlay and in distinct tracks), the constituents of the sugar moieties could be identified as apiose, glucose and rhamnose.

J. Planar Chromatogr. 31, 169-172 (2018). HPTLC for the analysis of enzyme activity of the purified β-glucosidase from Cyamopsis tetragonoloba with natural isoflavonoid (genistin and daidzin) and flavonoid O-glycoside (rutin, naringin and hesperidin) substrates on silica gel with toluene – ethyl acetate – acetone – formic acid 20:4:2:1 for genistin, daidzin and rutin, methanol – chloroform 3:17 for hesperidin and methanol – ethyl acetate 3:7 for naringin. Detection under UV 254 nm. The hRF values were 18 and 32 for daidzein and genistein, respectively. TLC was successfully used as the first step for validation of the metabolic product formation.