J. of Natural Products 61, 706-708 (1998). Isolation of novel flavonoid glycosides by prep. TLC on silica gel with the lower layer of chloroform - methanol - water 13:7:2. Final purification by prep. TLC with chloroform - methanol - water 80:20:3. Detection under UV 254 nm.

J. Planar Chromatogr. 12, 26-29 (1999). TLC of rutin on silica gel with ethyl acetate - formic acid - water 8:1:1, chamber saturation. Quantitation after drying by densitometry at 260 nm in reflectance mode, also at 440 nm after spraying with a 1% methanolic solution of 2-aminoethyldiphenylborinate.

Chromatographia 51, 473-477 (2000). Determination of retention-mobile phase composition relationships for 18 flavonoids chromatographed with non-aqueous systems of the type silica-diluent + polar modifier (S). Discussion of the slopes of the plots depending on the molecular structures of the solutes, primarily on the number of hydroxyl groups, and the selectivities and sequences depending on the diluent and modifier applied, with plots of Rm against [S] approximately linear.

J. Liq. Chrom. & Rel. Technol. 24, 1171-1179 (2001). TLC of genkwanin, tectochrysin, galangin, apigenin, pilloin, chrysin, kaempferol, pinocembrin, 5-hydroxy-4',7-dimethoxyflavone, and pinostrobin chalcone on silica gel with hexane - dichloromethane and dichloromethane - ethyl acetate in different proportions. Visualization under UV 254 and 366 nm and in day light after spraying with 1% methanolic iron(III) chloride solution or 1% methanolic aluminium chloride solution.

Proc. Intern. Symp. on Planar Separations Plan. Chrom. 305-307 (2003). TLC of flavonoids (7-O-glucoside luteolin, 7-O-glucoside apigenine, 5'-O-glucoside tricetin, 3-O-rhamnoside quercetin, 3-O-rhamnoside kaempferol, luteolin, quercetin, kaempferol, isoginkgetin, ginkgetin) on silica gel in a sandwich chamber, in a Personal OPLC chamber, and by planar electrochromatography method with 11 monocomponent, 8 binary phases in different concentration, and ternary and quaternary mobile phases. The best separation of flavonoids, biflavones, aglycones, glycosides was obtained with ethanol - ethyl acetate -dioxane - hexane (as solvent strength modifier).

leaves. J. Planar Chromatogr. 18, 244-248 (2005). Analytical and preparative TLC of coumarins from Peucedanum tauricum with bergapten, scopoletin, and coumarin A and B as standards on silica gel and on RP-2 with water - methanol 3:2 in horizontal chambers. Detection under UV light at 366 nm and densitometry at UV 366 and 320 nm. Re-chromatography with more selective mixtures of dichloromethane and acetonitrile 99:1 and 39:1. Identification by analytical co-chromatography with standards using mixtures of cyclohexane - ethyl acetate 3:1 and dichloromethane - acetonitrile 39:1.

J. Planar Chromatogr. 21, 83-88 (2008). TLC of (+)-catechin and (-)-epicatechin on silica gel with diisopropyl ether - formic acid 9:1 in an unsaturated twin-trough chamber. Detection by spraying with vanillin-sulfuric acid reagent followed by heating at 120 °C for 5 min. Quantitative determination by absorbance measurement at 490 nm.

CBS 107, 13-15 (2011). HPTLC of 5-hydroxymethylfurfural (HMF) in honey on silica gel, prewashed with methanol - water 6:1, with ethyl acetate. Quantitative determination by densitometry in absorbance mode at 290 nm. Optional detection by immersion in p-aminobenzoic acid reagent followed by heating at 110 °C for 5-10 min. The hRf value of HMF was 80. The calibration function was polynomial in the range of 0.8-80 ng/band whilst Michaelis Menten 2 regression was suitable for higher concentrations. The LOD of HMF in honey samples was 0.75 mg/kg (12 µL applied) and the LOQ 2.4 mg/kg. The method complies with the requirement of max. 15 mg/kg of HMF in honey. The results with this method were compared with those obtained by the spectrophotometric Winkler method and by HPLC-UV and mean differences were minor (3.3 % or 0.9 mg/kg). Complementary confirmation by HPTLC-MS online coupling. HMF zones identified under UV were eluted and analyzed by ESI-MS in full-scan and SIM mode.