in different_x000D_ growth stages by thin-layer chromatographic scanning. J. Planar Chromatogr. 31, 190-196 (2018). HPTLC of rupestonic acid (1), vitexicarpin (2), apigenin (3), and luteolin (4) in Artemisia rupestris on silica gel with chloroform – methanol – formic acid – water 650:63:17:7. Quantitative determination by absorbance measurement at 250 nm for (1) and 352 nm for (2) to (4). The hRf values for (1) to (4) were 56, 67, 34 and 18, respectively. Linearity was in the range of 128-725 ng/zone for (1), 70-400 ng/zone for (2), 26-145 ng/zone for (3) and 17-97 ng/zone for (4). The intermediate precision was below 5 % (n=6). Average recoveries for (1) to (4) were 100.0 %, 103.6 %, 98.1 % and 99.9 %.

Phytochem. Anal. 29, 452-462 (2018). HPTLC of apigenin(1), luteolin (2), kaempferol (3), quercetin (4), kaempferol‐3‐O‐glucoside (5), quercetin‐3‐O‐glucoside (6), isorhamnetin‐3‐O‐neohesperidoside (7) and rutin (8) in the fruits and aerial parts of Foeniculum vulgare (fennel), Pimpinella anisum (anise), Carum carvi (caraway), Cuminum cyminum (cumin), Coriandrum sativum (coriander), Apium graveolens (celery), Petroselinum crispum (parsley), and Anethum graveolens (dill) on silica gel with ethyl acetate – methanol – water – acetic acid 3:1:1:1. Quantitative determination by absorbance measurement at 254 nm. Heat maps and hierarchical clustering were performed for image processing. The hRF values for (1) to (8) were 97, 90, 83, 73, 30, 17, 7 and 2, respectively. LOD and LOQ were 54 and 166 ng/zone for (1), 52 and 157 ng/zone for (2), 58 and 177 ng/zone for (3), 37 and 112 ng/zone for (4), 57 and 172 ng/zone for (5), 54 and 164 ng/zone for (6), 55 and 169 ng/zone for (7) and 58 and 178 ng/zone for (8), respectively. The intermediate precision was <2 % (n=6). Average recovery was 98.4 % for (1), 92.3 % for (2), 95.3 % for (3), 96.8 % for (4), 94.5 % for (5), 95.6 % for (6), 95.1 % for (7) and 97.3 % for (8).

An interesting reagent for flavonoid determination.) Use of a methanolic solution of 1% aminoethanol diphenylborate and 5 % PEG 400 for detection of various flavonoids following TLC on silica. Fluorescent spots under 366 nm. Detection limit at ng range. Discussion of the correlation between the colour of the fluorescence, Rf values and structure.

Biochemical Systematics and Ecology 15, 581-586 (1987). Two-dimensional TLC of flavonoids on polyamide with toluene - methanol - MEK - n-butanol 30:20:15:0.3 (1st direction), water - n-butanol - acetone - dioxane 110:15:10:5 (2nd direction).

Planta 171, 88-95 (1987). TLC of flavonoid compounds on cellulose with chloroform - acetic acid 3:2 or 5% aq. acetic acid for glycosides and with water - acetic acid - 32 vol.% HCl 10:30:2 or 15% acetic acid for aglycones. Detection under UV 254 nm after spraying with NH3 and 2-amino ethyl-diphenyl-boric acid ester.

Phytochemistry 28, 303-305 (1989). TLC of 5-hydroxy-3,6,7,8,4'-pentamethoxy flavone, 5-hydroxy-3,6,7,8,3',4'-hexamethoxy flavone and 5,3'-dihydroxy-3,6,7,8,4'-pentamethoxy flavone on polyamide and silica with petrol ether (10-140°C) - toluene - butanol - methanol 12:6:1:1, toluene - dioxane - acetic acid 18:5:1. Detection under UV 366 nm before and after spraying with Naturstoffreagent A.

Chinese f. vanheurackii by thin-Layer chromatography.) (Chinese). J. Chinese Herb Med. (Zhongcaoyao) 21, 341-342 (1990). TLC of flavonoids on polyamide with 1) butanol – acetic acid – water 4:1:5, 2) 6:1:3. Detection by spraying with 1% aluminium chloride in ethanol, and observing under UV. Comparison of the main constituents of flavonoids between two sources.

J. Chromatogr. 552, 453-461 (1991). TLC of natural pyranocoumarins on silica or florisil with binary and ternary solvents containing a polar modifier. Detection under UV 254 nm. Investigation of the relationship between RM values and the concentration of the polar modifier in the eluent. Optimization of the conditions for the separation of the isomers. Correlation of retention parameters for silica and florisil with selectivety.