Anal. Sci. 31, 535-541 (2015). HPTLC of neochlorogenic acid (1), chlorogenic acid (2), cryptochlorogenic acid (3), caffeic acid (4), rutin (5), hyperin (6), isoquercetin (7), quercitrin (8) and quercetin (9) on silica gel with ethyl acetate - methanol - formic acid 15:1:2. Quantitative determination by absorbance measurement at 365 nm. The hRF values of (5), (6) and (8) were 14, 25 and 44. Linearity was in the range of 1.3-41.6 µg/mL for (1), 2.9-94.3 µg/mL for (2), 1.3-41.4 µg/mL for (3), 0.4-12.4 µg/mL for (4), 0.8-25.7 µg/mL for (5), 2.5-81.0 µg/mL for (6), 0.9-28.9 µg/mL for (7), 6.4-203.8 µg/mL for (8) and 0.7-23.5 µg/mL for (9). LOD and LOQ for (1) to (9) were in the range of 40-80 and 120-280 ng/mL, respectively. The intermediate/interday/intraday precision was 2.7 %. Recoveries for (1) to (9) ranged from 97 to 103 %.

J. of Chromatogr. Sci. 53 (2), 338-344 (2014). Presentation of a method for the simultaneous quantification of three glycosidic isoflavones (daidzin, genistin and glycitin) in soybean (Glycine max L.) by HPTLC on silica gel with toluene – ethyl acetate – formic acid – acetic acid 2:16:2:1. The hRf values of daidzin (1), genistin (2) and glycitin (3) were 39, 51 and 32, respectively. Detection and quantification by densitometry at 260 nm. Validation in accordance with the ICH guidelines with the results for precision of ≤2.1 %, ≤0.7 % and ≤0.1 %, recovery of 95.9-106.7 %, 86.9-106.6 % and 98.5-105.6 %, LOD of 3, 19 and 4 µg/mL and LOQ of 9, 59 and 11 µg/mL for the glycosidic forms of (1), (2), and (3). The method was used for the analysis of the soybean variety Kh-09 bragg which showed high amounts of glycosidic isoflavones: 278, 598 and 109 µg/g for (1), (2), and (3). After fermentation with Bacillus subtilis, the concentration of glycosidic isoflavones significantly decreased while those of the aglycone isoflavones increased.

J. Ethnopharmacol. 204, 26-35 (2017). HPTLC of quercitrin (1), isoquercitrin (2), quercitrin-3-O-β-D-xylopyranosyl-α-L-rhamnopyranoside (3) on silica gel with ethyl acetate – formic acid – acetic acid – water 100:11:11:26. Detection by spraying with NEU`s reagent (diphenylborinic acid 2-aminoethylester, natural product reagent), followed by drying at 110 °C for 2 min. Quantitative determination by absorbance measurement at 265 nm. LOD and LOQ were 31 ng/zone and 75 ng/zone for (2). Recovery was between 99.8 and 101.1 % for (2). Intermediate precision was <2 % (n=3).

J. Planar Chromatogr. 31, 129-134 (2018). HPTLC of hydroxysafflor yellow A in safflower on silica gel with 3.6 % hydrochloric acid – methanol – ethyl acetate 7:3:1. Quantitative determination by absorbance measurement at 399 nm. The hRf value for hydroxysafflor yellow A was 60. Linearity (starting from LOD) was in the range of 62-793 ng/zone with r=0.9991. The intermediate precision was below 3 % (n=6). The LOD and LOQ were 59 and 169 ng/zone, respectively. Recovery was between 96 and 102 %.

Braz. J. Pharmacog. 28, 631-639 (2018). HPTLC of 3-O-α-L-rhamnopyranosyl-(1→ 2)-α-L-arabinopyranosyl pomolic acid, 2,4,6-trihydroxy-4-methoxybenzophenone-2-O-β-d-glucoside, hyperoside, geniposidic acid, nicotiflorin, narcissin, randiasaponin IV and rutin in the leaves, stems and roots of Fadogia agrestis on silica gel with chloroform – ethyl acetate – methanol – formic acid 15:30:10:4. Detection by immersion in the anisaldehyde–sulfuric acid reagent, followed by heating at 100 ºC for 3 min. The HPTLC fingerprinting method was suitable for rapid decisive authentication and comparison of differences among samples of identical source.

( Chinese) - (TLC-densitometry of isoflavones in pueraria lobata.) TLC of daidzein, daidzin, puerarin and daidzein 4,7-diglucoside in the root of P. lobata and in tablets on silica with toluene - methanol -10 % formic acid 7:3:00.2, or ethyl acetate - methanol -50 % formic acid 8:2:0.2. Quantification by densitometry. Relative standard deviations: 1.51 % for puerarin, 1.61 % for daidzein.

Herba Hungarica 27, 27-33 (1988). TLC of the flavonoids 5,7,4-trihydrixy-flavane-glycoside, 7,4-dihydroxy- flavanol-diglycoside and taxifolin-3-glycoside on polyamide with ethanol - water 3:2. Detection with 5% aluminium chloride in ethanol. TLC of the aglycones and sugar moieties on silica impregnated with phosphat buffer (pH = 5) with n-butanol - acetone - phosphate buffer 40:40:10. Detection by spraying with aniline oxalate reagent.

Biochemical Systematics and Ecology 16, 43-46 (1988). TLC of lipophilic flavones (5,6,4'-trihydroxy-7,8,3'-trimethoxyflavone (thymonin) and 5,6,4'-trihydroxy-7,3'-dimethoxyflavone on silica with toluene - acetic acid 4:1, toluene - dioxane - acetic acid 90:25:4.