J. Chromatogr. Sci. 56, 518-523 (2018). Presentation of a validated method for the quantitation of dihydrokaempferol-4′-O-glucopyranoside. This flavanonol glucoside is a marker compound in the flower of Alcea rosea L. with significant antioxidant and anticancer activity against the HepG-2 cell line. HPTLC on silica gel with ethyl acetate – methanol – water – acetic acid 600:100:80:3 over 70 mm with chamber saturation for 30 min. Quantitative determination by absorbance measurement at 295 nm. The amount of dihydrokaempferol-4′-O-glucopyranoside in the flowers of A. rosea was 0.733 g/100 g and 0.928 g/100 g after maceration and sonication for 15 min, respectively. Linearity was in the concentration range of 0.9-3.6 µg/zone. The %RSD of the intra-day and inter-day precision was 0.2–1.5 % and 0.5–1.7 %, respectively. The LOD and LOQ were 312 ng/zone and 947 ng/zone, respectively.

Phytochemistry. 24, 1495- 1498 (1985). Two-dimensional TLC of extracts of phyllocladus species on cellulose with 1) t-butanol - acetic acid - water 3:1:1 and 2) acetic acid - water 6:94. Detection with vanillin-HCl reagent. Catechin, epicatechin and phylloflavan, a new flavanoid compound readily resolved.

Phytochemistry 25, 1719-1721 (1986).TLC of flavonoid sulfates on cellulose with butanol - acetic acid - water 3:1:1 or on polyamide with methanol - water -29 % NH3 10:9:1. Detection with 2-aminoethyldiphenylborinate (0.1 % in methanol). TLC of the hydrolysis products on polyamide with benzene - MEK - methanol 3:1:1. Also ion pairing HPLC. Specific analytical procedure for the detection of flavonoid sulfates in plant extracts.

Acta Pharm. Hungarica 57, 193-198 (1987). HPTLC of rutin, vitexin-4'- rhamnoside, hyperoside, vitexin and apigenin-7-glucoside on silica with ethyl acetate - formic acid - acetic acid - water 100:11:11:28 for glycosides, with toluene - ethyl acetate - acetic acid - water 30:50:16:4 for aglycones. Detection under UV 254 and 366 nm and spraying with 1% AlCl3 methanolic solution. Densitometry by absorbance at 400 nm.

Biochemical systematics and ecology 16, 479-483 (1988). 2-dimensional TLC of flavonoids on polyamide 6.6 with water - butanol - MEK - dioxane 70:15:10:5 in the first direction and dichloroethane - methanol - butanone - water 55:25:21:4 in the second. Visualization under UV 366 nm before and after spraying with a 0.1% solution of ß-aminoethyl diphenylborate in 50% methanol.

Biochemical Systematics and Ecology 17, 197-198 (1989). TLC of quercetin 3-O-rutinoside, quercetin 3-O-glucoside, kaempferol 3-O-rutinoside and kaempferol 3-O-gucoside on silica with ethyl acetate - formic acid - acetic acid - water 100:11:11:27 and on cellulose with 15% acetic acid.

Phytochemistry 30, 167-1675 (1991). 2D-TLC of complex flavonoid mixtures on polyamide with 1) toluene - dichloromethane - methanol - MEK - butanol 60:50:42:1 and 2) water - methanol - MEK - butanol 800:240:80:1, two migrations in each direction. Analysis of the hydrolysate sugars on silica impregnated with 0.2 M Na2PO4 with acetone - water 9:1. Detection with aniline malonate. TLC method suitable for other types of flavonoid glycosides.

J. of Natural Products 55, 1498-1504 (1992). TLC of isoflavonoids on RP-18 silica with acetonitrile - water - formic acid 40:60:1. Detection under UV 254 nm or with Pauley’s reagent consisting of sol. A (NaNO2 =.5%), sol. B (0.5% sulfanilic acid in 2% HCl) and sol. C (5% NaOH in 50% ethanol). Spraying with a mixture of sol. A and B 1:1, followed by separate spraying with sol. C. Excellent TLC conditions on RP-18 silica for the separation of isoflavonoids and related phenolic compounds.