J. Ethnopharmacol. 182, 101-109 (2016). HPTLC of quercetin (1) and chlorogenic acid (2) in the leaves of Moringa oleifera on silica gel with toluene – ethyl acetate – formic acid 5:4:1 for (1) and ethyl acetate – dichloromethane – formic acid – acetic acid – water 100:25:10:10:11 for (2). Quantitative determination by absorbance measurement at 375 nm for (1) and 330 nm for (2). The hRF values of (1) and (2) were 63 and 30, respectively.

Planta Medica 82 (17), 1475-1481 (2016). Four new flavone glycosides (acacetin group) isolated from Piper aduncum leaves were submitted to acidic hydrolysis; TLC of the aqueous fractions, purified with ethyl acetate, on silica gel and RP-18 with chloroform – methanol – water 30:20:3. Detection by spraying with sulfuric acid reagent, followed by heating. By comparison to standards (applied in overlay and in distinct tracks), the constituents of the sugar moieties could be identified as apiose, glucose and rhamnose.

J. Planar Chromatogr. 31, 169-172 (2018). HPTLC for the analysis of enzyme activity of the purified β-glucosidase from Cyamopsis tetragonoloba with natural isoflavonoid (genistin and daidzin) and flavonoid O-glycoside (rutin, naringin and hesperidin) substrates on silica gel with toluene – ethyl acetate – acetone – formic acid 20:4:2:1 for genistin, daidzin and rutin, methanol – chloroform 3:17 for hesperidin and methanol – ethyl acetate 3:7 for naringin. Detection under UV 254 nm. The hRF values were 18 and 32 for daidzein and genistein, respectively. TLC was successfully used as the first step for validation of the metabolic product formation.

and their herbal formulations. J. Planar Chromatogr. 31, 451-459 (2018). HPTLC of berberine chloride (1) and galangin (2) in Tinospora cordifolia M._x000D_ and Alpinia galanga on silica gel with toluene ‒ ethyl acetate ‒ formic acid 3:6:1. Quantitative determination by absorbance measurement at 267 nm. The hRF values for (1) and (2) were 17 and 82. Linearity was between 200 and 1200 ng/zone. LOD and LOQ were 28 and 86 ng/zone for (1) and 2 and 5 ng/zone for (2), respectively. The intermediate precision was <2 % (n=3). Recovery was between 97.5 and 102.6 % for (1) and (2).

Biochemical Systematics and Ecology 13, 391-402 (1985). TLC on silica with ethyl acetate - pyridine - water - methanol 16:4:2:1.

Quantitative HPTLC of silybin - a comparison to quantitative HPLC. GIT Suppl. Chromatographie 3, 4-11 (1987). With the aid of an optimized HPTLC procedure silybin was quantitatively determined after extraction with methanol in the drug cardui mariae, besides Silydianin, Silydristin and Taxifolin. The extraction procedure was simplified and optimized with a view to systematic errors. The statistical comparison of the results with those established by HPLC, shows the equivalence of both analytical methods. Under the aspect of the expenditures (time, personnel, appliances) as well as total operating costs HPTLC turnes to be a most appropriate method for this routine analysis.

Yellow spots in the potato variety „Hertha“. J. Lebensm. Unters. Forsch. 186, 407-411 (1988). TLC of flavonols on silica with toluene - chloroform - acetone 40:25:35, on polyamide with chloroform - MEK - methanol 60:26:14; of flavonolglycosides on silica with ethyl acetate - MEK - formic acid - H2O 50:30:15:15; of sugars on cellulose with pyridine - n-butanol - H2O 24:12:6 and of p-hydroxycinnamic acids on polyamide with toluene - ethyl formate - formic acid 50:40:10. Detection of sugars with naphthoresorcinol or anisidine and under UV; of flavonols under UV.

Phytochemistry 28, 87-981 (1989). TLC of pisatin and maackiain on silica with chloroform - methanol 4:1. Detection with vanillin - HCl reagent. Determination of RM: TLC on RP-18 254 plates activated by heating at 120°C for 15 min. with acetonitrile - water 13:7. Visualization under UV and by exposing to iodine vapor.