Biochemical Systematics and Ecology 22, 501-505 (1994). Two-dimensional TLC of flavonoids on polyamide with water - butanol - acetone - dioxane 70:15:10:5 for the first development and 1,2-dichloroethane - methanol - butanone - water 55:20:22:3 for the second. Visualization by spraying with 0.1% diphenylboric acid ethanolamine complex in methanol - water 1:1. Examination after 1 h under UV 366 nm.

J. Chinese Trad. Patent Med. (Zhongchengyao) 18 (5) 1-3 (1996). TLC on silica with chloroform - methanol - formic acid 9:1:1. Detection by spraying with 5 % AlCl3 in ethanol. Discussion of the optimum process parameters for extracting total flavone.

Phytochemistry 47, 273-280 (1998). Isolation of three new 2-prenyl-3-hydroxyflavanones by preparative TLC on silica with chloroform - ether 4:1, benzene - methanol 10:1, then benzene - ethyl acetate 3:1. Also isolation of a new 2-farnesyl-3-hydroxyflavanone by preparative TLC with benzene - acetone 4:1, chloroform - methanol 20:1 and finally hexane - acetone 4:1.

J. Planar Chromatogr. 11, 230-232 (1998). TLC of flavone derivatives (apigenin, apigenin-7-O-glucoside, luteolin, luteolin-4-O- and -7-O-glucoside) on RP-18 with methanol - water - acetic acid 25:25:3. Visualization by spraying with a methanolic solution of 1% diphenylboric acid aminoethyl ester (NP) and 5% polyethylene glycol (PEG). Preparative TLC was performed on conventional thick silica gel and RP-18 with ethyl acetate - formic acid - water 6:1:1 and methanol - water - acetic acid 25:25:3. The spots were analyzed by UV after extraction. Acid hydrolysis of the isolated flavonoids was carried out directly on the TLC plate after application by dipping (of the application zone) into 5 N HCl in methanol - water 1:1 and leaving at 110°C for 45 min to hydrolyze.

and Vaccinium vitis-idaea. L. J. Planar Chromatogr. 13, 101-105 (2000). HPTLC of flavonoids (i. a. quercetin, avicularin, hyperin, isoquercetin) on silica gel by two- and three-step gradient elution with mixtures of toluene, hexane, ethyl acetate and methanol for glycosides and multiple development for aglycones. Videodocumentation was achieved by use of videoscanning equipment. Visualization under UV 254 or 365 nm; or by spraying with one of the three reagents: (i) 10% KOH in methanol; (ii) 1% solution of FeCl3; and (iii) Gibbs reagent. Quantitation by densitometry at 254 nm.

Planta med. 65, 671-673 (1999). Preparative TLC of e.g., 3,4-dihydro-5-hydroxy-7-methoxy-4- (4'-methoxyphenyl)coumarin on silica gel with dichloromethane - hexane - ethyl acetate 6:3:1, of 3,4-dihydro-4-(4'-hydroxyphenyl)-5,7-dihydroxycoumarin, 3,4-dihydro-5-hydroxy-7-methoxy- 4-(4'-hydroxyphenyl)coumarin, and 3,4-dihydro-5,7-dihydroxy-4-(4'-methoxyphenyl)coumarin on silica gel with 1. dichloromethane - hexane - methanol 6:3:1 and 2. dichloromethane - methanol 9:1. Detection under UV.

J. Planar Chromatogr. 15, 433-436 (2002). TLC of (+)-catechin, (-)-epicatechin and (+/-)-catechin on cellulose, preferably prewashed with water - acetic acid 19:1, with 1-butanol - water - acetic acid 4:2:1. Detection by dipping in anisaldehyde - sulfuric acid reagent and with vanillin - phosphoric acid reagent. The work shows the importance of optimization of prewashing, pre-conditioning, and use of the correct development chamber and detection reagent in the TLC analysis procedure.

with and without red heartwood. J. Planar Chromatogr. 17, 350-354 (2004). TLC of (+)-catechin and (-)-epicatechin on silica gel with diisopropyl ether - formic acid 9:1. Detection by spraying with vanillin-sulfuric acid reagent and heating at 120 °C for 5 min. Quantitative determination by absorbance measurement at 513 nm.