Phytochemistry 22, 1421-1423 (1983). Two-dimensional TLC of flavonoid glycosides on polyamide with 1)toluene-methanol - butanone-water 70:35:10:4 and 2) water - butanol-acetone - dioxan 15:3:2:1. Detection by UV. Also column chromatography.

J. Agric. Food Chem. 33, 1153-1157 (1985). Two-dimensional TLC determination using a new photobiological assay technique for detection of furocoumarins - rapid and easy and extremely sensitive biological assay. Detection limit 10 -50 ng Two-dimensional TLC separation of furocoumarins (psoralen, angelicin, 5-MOP, 8-MOP, isopimpinellin) on silica with chloroform and hexane - pentane - ethyl acetate 35:35:30, using Escherichia coli as indicator microorganism.

Arzneim.-Forsch. Drug Res. 41, 481-483 (1991). TLC of phenolic procyanidins on silica with ethyl acetate - water - formic acid 90:5:5 and of peracetylated procyanidins with chloroform - ethyl acetate - acetone 7:2:1 and toluene - acetone 8:2. Detection: vanilline - hydrochloric acid, vanilline - sulfuric acid (120°C). Preparative TLC of anthocyanidins on cellulose with acetic acid - hydrochloric acid - water 30:3:10; Identification by UV.

J. Planar Chromatogr. 5, 169-174 (1992). TLC of furocoumarins (bergapten, imperatorin, phellopterin, xanthotoxin, isopimpinellin, isoimperatorin) on florisil or silica with 0-15% ethyl acetate in benzene or 5-10% diisopropylether in dichloromethane - hexane 7:3; one- and two-dimensional development.

J. Planar Chromatogr. 23, 40-45 (2010). HPTLC of flavonoids (vitexin, isovitexin, orientin, isoorientin, rutin) on polyamide at 23 +/- 2°C and 40 % relative humidity with the three-component mobile phase A-B-C 33:67:8, where A is dodecyl sulfate - n-butanol - n-heptane 147:158:28, B is water, and C is formic acid. The solvent mentioned gave the best resolution of vitexin (hRf 61), isovitexin (hRf 21), orientin (hRf 28), isoorientin (hRf 34), and rutin (hRf 52). Detection by spraying with 1 % aluminium trichloride in ethanol followed by waiting for approximately 1h. Quantitative determination by fluorescence measurement at 366 nm. Precision was found to be 0.98, 0.91, 1.02, 1.04 and 0.87 % for isovitexin, rutin, orientin, isoorientin, and vitexin, respectively. Average recoveries (at three different concentrations) were between 98.9 and 100.6 % from P. pubescens, P. glauca and P. yixingensis.

J. Chromatogr. A 1289, 105-118 (2013). HPTLC of 11 anthocyanes named cyanidin (1), delphinidin (2), malvidin (3), peonidin (4), pelargonidin (5), cyanidin-3-glucoside (6), delphinidin-3-glucoside (7), malvidin-3-glucoside (8), peonidin-3-glucoside (9), pelargonidin-3-glucoside (10) and malvidin-3,5-diglucoside (11) in pomace, feed, juice and wine on silica gel with ethyl acetate - toluene - formic acid - water 50:15:4:6 for the anthocyanidins (1) to (5) and ethyl acetate - 2-butanone - formic acid - water 35:15:6:4 for the anthocyanins (6) to (11). Quantitative determination by absorbance measurement using a multi-wavelength scan at 505 nm for (10), 510 nm for (5), 520 nm for (4) and (9), 530 nm for (1), (3), (6), (8) and (11) and 555 nm for (7). Detection was compared by dipping into a DPPH radical reagent solution (0.5 mM methanolic solution of the DPPH) and into Aliivibrio fischeri bioassay suspension. Linearity was in the range of 180-540 ng/zone for (1), 47-141 ng/zone for (2), 147-441 ng/zone for (3), 24-89 ng/zone for (4), 32-95 ng/zone for (5), 71-343 ng/zone for (6), 63-304 ng/zone for (7), 40-191 ng/zone for (8), 27-131 ng/zone for (9), 16-76 ng/zone for (10) and 45-219 ng/zone for (11). The intermediate precision over several months was below 6.7 % (n=3). The LODs of the anthocyanidins were much better compared to those for anthocyanins. The LOQs for (1) to (11) were below 90 ng/zone, most even below 30 ng/zone and for (9) and (10), the LOQ were even below 7 ng/zone. Radical scavenging as well as bioactivity properties were important complementary detection methods.

J. Food Comp. Anal. 41, 174-180 (2015). HPTLC fingerprint of red wines on silica gel with ethyl acetate - formic acid - acetic acid - water 10:1:1:2. Detection by dipping into NP reagent (1 g of diphenylborinic acid aminoethylester was dissolved in 200 mL ethyl acetate), followed by drying in cold air and dipping in PEG reagent (10 g of polyethylene glycol 400 is dissolved in 200 mL dichloromethane). Qualitative identification under UV 254 and 366 nm. Precisions of hRF values (% RSD) of three selected zones on two plates was below 0.02 %.

J. Ethnopharmacol. 194, 30-56 (2016). Review of information on the ethnobotany, phytochemistry, pharmacological research and toxicity of Anogeissus species, including the application of HPTLC for the quantitative investigation of gallic acid, ellagoc acid as well as flavonoids like quercetin and rutin.