Synthesis of 3-O-methyl-L-xylose. Acta Chim. 124, 315-321 (1987). Elucidation of the structures of synthesized compounds by TLC (and 13C-NMR spectroscopy). TLC on silica with dichloromethane - methanol 95:5, dichloromethane - ethyl acetate 9:1, dichloromethane - acetone 9:1, dichloromethane - methanol 85:15. Detection by charring with 50% sulfuric acid or under UV.

J. Planar Chromatogr. 3, 173-176 (1990). Improvement of a TLC method: Rapid quantitative HPTLC of glucose, fructose, and other common sugars on silica by single or multiple developments with acetonitrile - water 17:3 containing various amounts of 2-aminoethyl diphenylborate (NST). Best results with a solvent containing 1,5 mmol/1 NST, 5 % methanol, and phosphate buffer (pH 5.5) instead of water. Time requirement for 18 samples of glucose, fructose, and saccarose including manual application and evaluation 45 min. Detection by immersion of the dried plates for 10 s in DAP (2 % diphenylamine and 2 % aniline in methanol - 85 % phosphoric acid 8:2 and heating at 120 °C for 10 min. Quantification by densitometry (absorbance at 440 and 515 nm).

J. Planar Chromatogr. 11, 38-42 (1998). HPTLC of linamarin on silica gel (prewashed with methanol) with ethyl acetate - acetone - water 4:5:1 for 30 mm; then ethyl acetate - formic acid - water 6:1:1 to a distance of 85 mm. Developments were performed in an unsaturated chamber. Visualization by dipping into a solution of aniline (2%), diphenylamine (2%), and phosphoric acid 15% in acetone, followed by heating at 105°C for 60 min. Quantitation by densitometry at 525 nm. Simple, quick and accurate quantitative HPTLC procedure.

J. Liq. Chrom. & Rel. Technol. 26, 2729-2739 (2003). HPTLC of sucralose® (4-chloro-4-deoxy-a-D-galactopyranosyl-1,6-dichloro-1,6-dideoxy-b-D-fructofuranoside) on amino-bonded silica gel with acetonitrile - water 4:1. Subsequently, the wet plate was heated for 20 min at 190°C in an oven resulting in the formation of a brilliant fluorescent spot. Inspection under UV 365 nm directly or after dipping for 5 s in a cetyl-trimethylammonium bromide solution (5 g in 100 mL methanol), which resulted in fluorescent enhancement by a factor of two. Quantitation by measurement either in the absorption or fluorescence mode. Excellent repeatability (RSD = 3.4%) and recovery (95%).

J. Planar Chromatogr. 24, 211-213 (2011). HPTLC of exo-polysaccharides from bacterial fermentation and a series of mono-, oligo-, and polysaccharide reference solutions on silica gel with chloroform - toluene - 35 % formic acid - methanol 10:2:2:7 in a twin-trough chamber saturated for 1 h. Detection of starch-type polysaccharides by placing the plate into a twin-trough chamber saturated with iodine vapor. After drying determination by densitometry in absorbance mode at 400 nm. The plates were then immersed for 1 min in a solution of 10 % concentrated sulfuric acid in n-propanol - toluene 1:1, followed by heating at 115 °C for 10 min. The zones were evaluated visually and densitometrically at 400 nm.

Herba Hungarica 28, 87-91 (1989). TLC of glucose, galactose and mannose on silica with butanol - pyridine - benzene - water 5:4:3:1. Visualization with p-anisidine phtalate solution. Two-dimensional TLC of alanine, glycine and threonine on silica with butanol - acetic acid - water 4:1:1 and phenol - water 75:5. Visualization by spraying with ninhydrin.

J. Chromatogr. A 1477, 108-113 (2016). In order to study the kinetics of invertase, a specific fructofuranosidase was cloned from the Leishmania major genome. Determination of the kinetic parameters of the β-D-fructofuranosidase by TLC on silica gel impregnated with sodium bisulfate and citrate, developed successively twice with acetonitrile – water 4:1. Detection of the three carbohydrates sucrose, glucose and fructose by dipping the plates in a solution of 4-aminobenzoic acid. Quantitative determination by UV-densitometry. Description of the hydrolysis of sucrose by the Michaelis-Menten kinetic parameters (KM, Vmax) equal to 63.1 ± 7.6 mM, 0.04 ± 0.001 mM/min using glucose production and 83.0 ± 14.4 mM, 0.03 ± 0.002 mM/min monitoring fructose. Also, comparison of hydrolyses of three alternative substrates, raffinose, stachyose and inulin, and characterization of the regiospecificity of the reaction. The method proved to be suitable for the refined kinetic analysis of different reactions related to the hydrolysis of sugars.

nauk. SSSR, ser. chim. 1170-1177 (1983). (Russian). (Synthesis of polysaccharides. 13. Synthesis of 1,3-glucane). TLC of glucopyranose derivatives on silica with a) chloroform - dimethylketone 8:2, 95:5, 8:2, b) ethyl acetate - ethanol 9:1, c) benzene - ether 7:3.