The effect of this impregnation on the separation of sugars by TLC. J. Planar Chromatogr. 12, 190-195 (1999). Separation of some neutral sugars (aldopentoses, methylaldopentoses, aldohexoses, ketohexoses) on silica gel impregnated with sulfates, chlorides and nitrates of various metals and study of the effect of pH and of the concentration of the impregnating solutions. TLC on silica gel with n-propanol - chloroform - water 7:4:1, using stepwise development in an N-type saturated chamber. Detection by spraying with aniline-diphenylamine solution.

Biochem. Biophys. Res. Commun. 378, 890-894 (2009). TLC of 3‘-Sialyllactose and 6‘-Sialyllactose from bovine collostrum, before and after amidation with 2-(2-pyridilamino)ethylamine dihydrochloride (PAEA) on silica gel with tetrahydrofuran – acetonitrile – n-propanol – ammonium acetate 0.6M – 28 % ammonia solution 5:10:50:35+1 drop of ammonia. TLC of ganglioside GM3 from the whole brain of a minke whale, before and after PAEA amidation on silica gel with chloroform – methanol – calcium chloride 0.2 % 6:4:1. Detection by spraying with resorcinol-hydrochloric acid.

J. Planar Chromatogr. 27, 245-250 (2014). HPTLC of fructooligosaccharides DP3-DP9 in Morinda officinalis on silica gel with n-butanol - isopropanol - water - acetic acid 7:5:2:1. Detection by spraying with 1-naphthol–sulfuric acid reagent, followed by heating at 110 ºC for 3 min. Quantitative determination by absorbance measurement at 585 nm. The hRF values for DP3 to DP9 were between 20 and 70. Linearity was in the range of 150-1050 ng/zone for DP3, 137-962 ng/zone for DP4 and DP5, 131-919 ng/zone for DP6 and DP9, 144-862 ng/zone for DP7 and 156-1094 ng/zone for DP8. The intermediate/interday/intra-day precisions were below 5 % (n=6). Average recoveries for DP3 to DP9 were between 91.4 and 99.0 %.

CBS 117, 5-7 (2016). HPTLC of amylase-produced dextrins and standards glucose, maltose, maltotriose, maltotetrose, maltopentose, maltohexose, and maltoheptose on silica gel with acetonitrile – acetone – water 3:3:2 with chamber saturation to a migration distance of 60 mm. Detection by immersion into aniline-diphenylamine-phosphoric acid reagent (2 g diphenylamine and 2 mL aniline in 80 mL methanol in 10 mL 85 % phosphoric acid, made up to 100 mL with methanol) followed by heating at 120 °C for 5 min. Evaluation under white light. Quantitative determination by absorbance measurement at 500 nm. Evaluation in the polynomial working range of 40-210 ng/band.

Planta 172, 114-120 (1987). TLC of glucose, fructose, maltose on silica with isopropanol - acetone - water 45:30:25. Detection reagent: 4 g diphenylamine, 20 mL aniline, 30 mL phosphoric acid in 200 mL acetone. After spraying, heating for 15 min at 100 °C.

Don. grown in Egypt. Herba Hungarica 30, 98-104 (1991). TLC of rhamnose, xylose, arabinose, mannose, glucose, galactose, glucuronic acid and galacturonic acid on silica impregnated with 0.1M sodium sulfate developed with ethyl acetate – acetic acid – methanol – water 12:3:3:2. Visualization by spraying with thymol – sulfuric acid reagent.

M. Alexandria hydrolyzing potentially aromatic disaccharide glycosides. J. Agric. Food Chem. 46, 2748-2753 (1998). TLC of hydrolysis products of glycosides (i.a. glucose, rhamnose, rutinose, pNP-xyloside, eugenylprimeveroside) on silica gel with ethyl acetate - 2-propanol - water 65:30:10. Detection of sugars with 0.2% naphthoresorcinol in sulfuric acid - ethanol 1:19 and heating at 100°C for 10 min.

J. Planar Chromatogr. 16, 374-376 (2003). HPTLC of mannose and lactulose from urine on amino-modified silica gel with acetonitrile - water 7:3. After drying the plates were heated at 170°C for 20 min. Determination of lactulose by densitometry at 366 nm. Visualization after dipping into paraffin - hexane 1:2, and drying for 5 min at 100°C. The plate was immersed in three detection reagents: 1 L 0.1 mol/L AgNO3 and 200 mL acetone for 2 s, drying in air; 4 g NaOH in 4 mL water and 200 mL methanol for 2 s, drying for 2 min at 100°C; 20 g sodium thiosulfate dissolved in 100 mL water and 100 mL ethanol for 6 s, drying for 2 min at 100°C. Determination of mannitol by densitometry in absorbance mode at 660 nm.