J. Chinese Trad. Patent Med. (Zhongchengyao) 24 (4), 296-298 (2002). TLC on silica gel with iso-pentanol - pyridine - water 4:4:1. Detection by spraying with aniline - diphenylamine phosphate solution and heating at 105°C. Quantitation by densitometry at 380 nm.

J. Chromatogr. A 1272, 132-135 (2013). Honey is a saturated solution of sugars, used for a long time as a natural source of sugars and is an important ingredient in traditional medicine due to its antimicrobial, anti-inflammatory and antioxidant effects. For quality control and detection of adulteration, TLC of (1) glucose, (2) fructose and (3) sucrose on silica gel aluminum foil twice with ethyl acetate – pyridine – water – acetic acid 12:6:2:1 up to 80 mm. Detection in visible light after dipping in diphenylamine - aniline reagent (2 mg diphenylamine and 2 mg aniline HCl were dissolved in 80 mL acetone, 15 mL phosphoric acid were added and the solution was diluted to 100 mL with acetone) and drying at 110 °C for 15 min. The hRf value of (1) was 46, (2) 52 and (3) 39. Linearity was between 6-16 µg/zone for (1)-(3). Repeatability and intermediate precision (%RSD, n=6) for (1), (2), and (3) was 1.2, 1.3, and 1.1 % and 0.1, 1.3, and 1.1 %, respectively. Recovery (by standard addition) was between 100.7 and 102.1 % for all three substances.

II. Commercial chewing gum. Z. Lebensm. Unters. Forsch. 176, 12-15 (1983). TLC of sugars, polyols, coloring matters, waxes and paraffins, glycerides and polyvinyl derivatives, flavoring matters, on silica and cellulose with 7 solvent systems, e.g. butanol - isopropanol - water 5:3:1, isopropanol -ethyl acetate - water 7:2:1, acetone - water 9:1. Detection with 6 reagent mixtures.

J. Fac. of Sci and Tech. Kinki Univ. 20, 121-126 (1984). TLC of carbohydrates on silica with butanol - acetone - water in various proportions. Detection by spraying with 5-hydroxy-1-tetralone in sulfuric acid and UV irradiation.

J. Chinese Chromatogr. 8, 198, 158 (1990). TLC of glucose and mannose in the hydrolysate of Konjaku on silica, impregnated with 0.5 mol/L sodium dihydrogen phosphate, with isopropanol – acetone – 0.1 mol/L formic acid 4:4:2. Detection by spraying with diphenylamine – phosphoric acid – phenylamine – acetone – biphenylamine – acetic acid 4:20:2:200:0.66:100 (W/V/V/V/W/V/). Quantification by densitometry.

J. Chromatogr. A 705, 89-103 (1995). A review with 89 references on the title method and its applications. The technique separates fluorescently-labeled carbohydrates on polyacrylamide gels and uses a charge-coupled device camera to detect and quantify the products.

Rapid Commun. Mass Spectrom. 25, 2201-2208 (2011). HPTLC of an equimolar mixture of malto-oligosaccharides, derivatized with p-aminobenzoic acid, on silica gel with acetonitrile – water – acetic acid 8:2:1. Quantitative determination by fluorescence measurement at 366 nm. The relative molar composition of the oligomers, determined by HPTLC, was used as a reference data for mass spectrometric analyses. For both electrospray ionization and matrix-assisted laser desorption/ionization methods, the instrumental parameters significantly influence the signal intensities and areas.

Planta Medica 82 (17), 1475-1481 (2016). Four new flavone glycosides (acacetin group) isolated from Piper aduncum leaves were submitted to acidic hydrolysis; TLC of the aqueous fractions, purified with ethyl acetate, on silica gel and RP-18 with chloroform – methanol – water 30:20:3. Detection by spraying with sulfuric acid reagent, followed by heating. By comparison to standards (applied in overlay and in distinct tracks), the constituents of the sugar moieties could be identified as apiose, glucose and rhamnose.