Food. Res. Int. 102, 282-290 (2017). HPTLC of fructooligosaccharides in pure orange juice on silica gel with n-butanol – 2-propanol – water 10:5:4. Detection by spraying with a mixture of phosphoric acid (6.8 mL), urea (3 g) and ethanol (8 mL) in 100 mL of 1-butanol – water 8:1, followed by heating at 120 °C for 10 min. Quantitative determination by absorbance measurement at 450 nm.

JAOAC Int. 83, 675-678 (2000). TLC of mono- and disaccharides (with D(-)-fructose and D(+)-sucrose as standards) before and after hydrolysis - (30 min with 1 % oxalic acid in a boiling water bath) - on silica gel with acetonitrile-diluted phosphate buffer solution pH 5.5 17:3. Detection by immersion of the plate for 1 s into a solution of 2 mL aniline and 2 g of diphenylamine in 100 mL methanol and 15 mL o-phosphoric acid (85 %) and heating for 10 min at 120°C. Quantitation by densitometry at 560 nm in absorption/transmission mode. Improved and simple method for the determination of fructan inulin in yoghurts, honey cakes and chocolates.

J. Chromatogr. A 1110 (1-2), 214-221 (2006). Presentation of a rapid, simple and inexpensive method for the analysis of fructooligosaccharides as feed additives (prebiotics) in complicated biological samples with minimal pre-treatment. TLC of fructooligosaccharides in dietetic products and in samples from intestinal tract of monogastric animals on silica gel (impregnated with sodium acetate) with butanol - ethanol - water 5:3:2 with chamber saturation. Detection by spraying with diphenylamine - aniline - phosphoric acid in acetone. Quantitative determination by absorbance measurement at 370 nm.

Acta Chimica 110, 197-202 (1982). TLC of ferric-fructose (FeCl3), ferric-fructose (FeNO3), ferric-sorbose (FeCl3 ) on silica with butanol - acetone - HCl - acetylacetone - water 60:40:1:0.5:20. Detection with 10 % sulfuric acid and 2 % K4FeCN9.

nauk. SSSR, serija chimicheskaja 2400-2402 (1983). (Russian) (Synthesis of 3,4-di-o-benzyl-1,2,-o- (1-cyano)-ethylidcine-alpha -D-xylopyranose.) TLC of the title compound on silica with a) ether - benzene 1:1, 1:2; b) chloroform - methanol 93:7. Detection with 25 % sulfuric acid.

J.A.O.A.C. 72, 27,29 (1989). TLC separation of polysorbates (20, 40, 60, 65, 80) on silica with chloroform - methanol - acetic acid - water 68:12:12:3, propanol - chloroform - methanol - NH3 10:10:5:2. Visualization with cobalt thiocyanate reagent.

Var. australis (Diels) Rehd.) (Chinese). Chinese J. Herb Med. (Zhongcaoyao) 24, 563-566(1993). Preparative TLC of saponins and monosaccharides on silica with the lower phases of a) chloroform - methanol - water 16:5:1, and b) a solution of 9 mL of the above mixture blended with 1 mL acetic acid. Detection by spraying with 50% sulfuric acid in methanol for saponins and with naphthyl alcohol - sulfuric acid reagent for saccharides. Identification by MS and NMR.

J. Planar Chromatogr. 18, 285-289 (2005). HPTLC of glucose - after hydrolyis of starch using alpha-amylase and amyloglucosidase - on silica gel, pre-washed with methanol, treated by immersion in a 0.1 M solution of di-potassium hydrogen phosphate in methanol and activated for 30 min at 120 °C. Three-fold development was performed in a horizontal development chamber with acetonitrile - water 17:3. Detection by dipping in aniline-diphenylamine reagent, densitometry at 520 nm. Calibration was linear between 100 and 300 ng with a coefficient of determination r2 of 0.9959. The limits of detection and quantification for starch as glucose were 0.26 and 0.51 g/100 g, respectively.