J. Chromatogr. 558, 328-339 (1991). TLC of carbohydrates on silica developed twice with chloroform – acetic acid – water. Detection by spraying with a 50 % solution of sulfuric acid in ethanol followed by heating at 150 °C for 10 min.

J. Planar Chromatogr. 14, 61-62 (2001). HPTLC of glucose and maltose on silica gel, prewashed with dichloromethane - methanol 1:1, with ethyl acetate - acetic acid - methanol - water 12:3:3:2 in a saturated chamber at 24°C and a humidity of 45%. Visualization by spraying with a-naphthol - sulfuric acid reagent and heating at 110°C for approx. 5 min. Quantitative densitometry at 515 nm.

Acta Chromatographica 9, 79-86 (1999). HPTLC (TLC) of glucose, maltose, sucrose and trehalose on silica gel with acetonitrile - water 17:3 and ethyl acetate – acetic acid – methanol – water 12:3:3:2 for three times with chamber saturation; on amino plates with ethyl acetate – pyridine – water – acetic acid 12:6:2:1 and on cellulose plates with ethyl acetate – pyridine – water 2:1:2, both in a non-equilibrated chamber; on RP-18 with tetrahydrofuran – water 44:6 in a pre-equilibrated chamber. All plates were prewashed, e.g. by chromatography with dichloromethane - methanol 1:1. Detection by 4-aminobenzoic acid reagent, 1-naphthol–sulfuric acid reagent or aniline–DPA reagent, each followed by heating at 110 °C for 10 min. The combined results from comparison of hRf values for two different mobile phases on silica gel, on cellulose, amino phase and C18-bonded layers with diverse separation mechanisms, spiking experiments on silica gel, detection with selective reagents, and GC–MS analysis definitely proved the presence of maltose and glucose and the absence of trehalose in digestive gland–gonad complex and hemolymph samples from B. glabrata. Earlier papers reporting the presence of trehalose were undoubtedly in error.

J. Planar Chromatogr. 27, 19-22 (2014). HPTLC of maltose and fructose, dextrose, galactose and mannose on silica gel with ethyl acetate - propionic acid 1:1. Detection by dipping into orcinol solution (250 mg orcinol, 7.5 mL conc. sulfuric acid, 200 mL ethanol, and 50 mL water), followed by heating at 110 ºC for 5-10 min. Quantitative determination by absorbance measurement. The LOD for fructose, dextrose and mannose was 8 μg/zone and for maltose and galactose 2 μg/zone. Selective separation of maltose from other sugars was achieved using a mobile phase more suitable for the environment.

J. Chromatogr. 312, 492-496 (1984). TLC of oligo (D-galactosiduronic acids) obtained by hydrolysis of pectic acid with trichoderma reesei polygalacturonase on microcrystalline cellulose with ethyl acetate - butanol - formic acid-water 1:3:5:2. Detection by spraying with saturated aqueous solution of lead acetate or by dipping the plate in aniline phthalate reagent in acetone. One step development brought about the separation of the monomer and oligomers up to DP 5 within 1 h. With 3 runs oligomers up to DP 9 could be resolved.

J. Biochem. (Tokyo) 102, 291-296 (1987). Description of a method for immobilizing oligosaccharides on TLC plates for immunostaining. HPTLC of oligosaccharides on polyamide or amino-bonded silica and covalent linking to the plate by reductive amination with NaBH3CN. Detection by enzyme immunostaining using NeuGC-specific chicken anti-NeuGC-Lac Cer etc. Detection limit, 0.8 nmol of NeuGC-containing oligosaccharides.

J. Planar Chromatogr. 4, 77-79 (1991). HPTLC of xylose, 3-o-methylglucose and rhamnose on silica with ethyl acetate – pyridine – acetic acid – water 75:15;10:10 (3 consecutive runs); detection by immersion in amino-benzoic acid reagent. Detection by densitometry in reflectance mode at 400 nm. Determination of method accuracy for 0.4 and 0.8 µg/4 µL of each monosaccharides (mean results of 20 replicate analyses are reported); CV = < 2.7%.

Z. Lebensm. Unters. Forsch. A 206, 175-178 (1998). HPTLC of oligosaccharides (e.g. glucose, maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose) on silica gel with acetonitrile - 0.02 phosphate buffer 7: 3; the best resolution was achieved after the third development of the plate. Detection by dipping into diphenylalanine-aniline-phosphoric acid reagent, drying and heating to 120°C for 10 min. Densitometry.