J. Americ. Oil Chem. Soc. 60, 563-566 (1983). TLC of aflatoxins B1, B2, G1, G2 on silica with a) chloroform - methanol 100:3, b) toluene - ethyl acetate - formic acid 6:3:1 and 3 more solvents for specific extraction methods. Detection by UV or treatment with trifluoroacetic acid and spraying with 25 % sulfuric acid. Detection limit 2 ppb.

J. Chromatogr. 291, 219-230 (1984). TLC of antibiotics of the bleomycin group on silica with 0.19 M diammonium hydrogen phosphate - methanol 1:1, on silanized silica with 0.357 M ammonium nitrate - methanol 7:3 or 0.1 M hexane sulfonic acid - methanol 1:1. Detection with ninhydrin or by direct bioautography using bacillus subtilis. Also HPLC.

(Biological-physiological procedures of detection in thin-layer-chromatography procedures after manual transfer.) GIT-Suppl. 3, 79-87 (1986). Survey of indirect detection methods using bio-detectors, which are often more sensitive than known chemical reactions. Application for the detection of substances with antibiotics activity. Active plant hormones, pesticides, mycotoxins and compounds with cytostatic activity. Useful technique: Place developed plate on agar or gelatine sheet containing the antibiotic allyactive compound.

Chinese J. Pharm. Anal. (Yaowu Fenxi Zazhi) 13, 407-408 (1993). Review.

J. Liq. Chromatogr. Relat. Technol. 31, 1903-1912 (2008). TLC of cefacetril on silica gel with methanol - acetonitrile in a sandwich chamber. The developed TLC plates were dried, then immersed briefly in the microorganism solution, and incubated for 20 h at 37 °C. After incubation the plates were sprayed with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide solution and left at room temperature for 30 min. White inhibition zones were observed against a purple background (Chrom Biodip Antibiotic Test kit). The described procedure is suitable for screening and semiquantitative determination of antibiotic residues in milk.

J. Planar Chromatogr. 24, 524-528 (2011). TLC of cefixime on silica gel with toluene - ethyl acetate - formic acid - water 5:29:11:5 with chamber saturation for 30 min at 25 +/- 2 °C. The hRf value was 54. Quantitative determination by densitometry in absorbance mode at 293 nm. Linearity was between 500 and 1500 ng. The repeatability (%RSD) was below 2 %. The limit of detection and the limit of quantification was 9 and 42 ng/zone, respectively. The recovery was between 98.9-100.3 %.

J. Liq. Chromatogr. Relat. Technol. 37, 2420-2432 (2014). HPTLC of lamivudine (1) and zidovudine (2) on silica gel with ethyl acetate – hexane – methanol – acetic acid 40:40:20:1. Quantitative determination by absorbance measurement at 278 nm. The hRF values for (1) and (2) were 25 and 73, respectively. Linearity was in the range of 100-600 ng/zone for (1) and 200-1200 ng/zone for (2). The intermediate/interday/intra-day precisions were below 2 % (n=3). The LODs and LOQs were 5 and 15 ng/zone for (1) and 10 and 32 ng/zone for (2), respectively. Recovery was between 95 and 105 %.

J. AOAC Int. 98, 871-875 (2015). TLC direct bioautography of 18 thyme (Thymus) specimens and species on silica gel with methanol - water 27:73. Bioautography by dipping into a suspension of Bacillus subtilis for 8 s, followed by incubation for 17 h at 37 °C. The plates were sprayed with 0.2 % aqueous solution of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and incubated for 30 min at 37 °C. Thymus citriodorus and Thymus marschallianus were selected as promising targets for further investigations.