strain S24. Arzneim.-Forsch./Drug Res. 56, 171-179 (2007). TLC of SJA-95 and mycosamine on silica gel with butanol - acetic acid - water - tetrahydrofuran 6:2:2:1 and butanol - acetic acid - water - dioxane 6:2:2:1. Detection under UV 254 nm and under white light after spraying with ninhydrin reagent.

J. Liq. Chromatogr. Relat. Technol. 33, 894-902 (2010). Five amphoteric piperazynyl fluoroquinolones and flumequine were analyzed in an RP system on C8 plates with acetonitrile/aqueous acidic mobile phases containing various concentrations of potassium perchlorate. Perchlorate as a so-called chaotropic ion caused the increase in the retention of basic fluoroquinolones. TLC of sarafloxacin, difloxacin, norfloxacin, enrofloxacin, ciprofloxacin, and flumequine on RP-8 with acetonitrile - water containing constant concentration of citric acid but various concentrations of potassium perchorate. After air-drying, fluoroquinolone zones were detected at 366 nm and 254 nm and flumequine only at 254 nm. The retention increased with the increasing concentrations of perchlorate ion in the mobile phase and achieved a plateau for the mobile phases containing 10-20 mM of chaotropic perchlorate.

J. Planar Chromatogr. 26, 56-61 (2013). TLC of ofloxacin (1) and dexamethasone (2) on silica gel with methanol - 0.01 M phosphate buffer 2:3 and pH adjusted to 5 with orthophosphoric acid. Quantitative determination by absorbance measurement at 300 nm for (1) and 240 nm for (2). The hRf values for (1) and (2) were 22 and 60, respectively. Linearity was in the range of 1-6 µg/zone for both (1) and (2). The LOD and LOQ were 260 and 870 ng/zone for (1) and 220 and 740 ng/zone for (2), respectively. Average recovery (by standard addition) for (1) and (2) was 99.2 %. Intermediate/interday/intra-day precision was below 2 % (n=3). The method showed comparable results with a validated HPLC method.

Food Addit. Contam. 31, 1959-1967 (2014). TLC of (1) sulfamethoxypyridazine-fluorescein isothiocyanate and (2) sarafloxacin-Texas Red on silica gel with chloroform - methanol 4:1 for (1) and methanol - ethyl acetate - ammonia water 4:1:1 for (2). Fluorescence polarisation was measured at 485/>530 nm (emission cutoff at 515 nm, G factor 1.0) for (1), and 585/>620 nm (emission cut-off at 610 nm, G factor 1.0) for (2). The hRF values for (1) and (2) were 10 and 80, respectively. The method allowed the synthesis of fluorescent tracers that bind recombinant bi-specific single-chain diabodies to detect fluoroquinolones and sulfonamides in milk.

J. Planar Chromatogr. 29, 469-473 (2016). HPTLC of miconazole in creams on silica gel with ethyl acetate ‒ 25 % ammonia 50:1. Quantitative determination by absorbance measurement at 228 nm. The hRF value for miconazole was 57. Linearity was between 1250 and 3000 ng/zone. The intermediate precisions were below 2 % (n=6). Recovery ranged between 98.0 and 106.9 %.

J. AOAC Int. 101, 385-393 (2018). HPTLC of nifuroxazide and its impurities A, B, C and D on silica gel with ethyl acetate – acetone – methanol – ammonia 170:50:10:1. Quantitative determination by absorbance measurement at 230 nm. The hRF values for nifuroxazide and its impurities A, B, C and D were 67, 46, 86, 8 and 34, respectively. Linearity ranged between 10-100 μg/zone for nifuroxazide and 0.1-1.0 μg/zone for impurities A to D. LOD and LOQ were 28 and 84 ng/zone for A, 27 and 80 ng/zone for B, 24 and 73 ng/zone for C and 26 and 79 ng/zone for D. The intermediate precision was <1 % (n=3). Average recovery was 100.5 % for nifuroxazide, 100.2 % for A, 99.0 % for B, 100.0 % for C and 100.2 % for D.

Analyst 109, 1331-1333 (1984). TLC of 4 ionophores monensin, narasin, salinomycin and lasalocid on alumina with freshly-prepared ethyl acetate - water 100:3. Detection by spraying with vanillin reagent or, for low levels, by bioautography.

J.A.O.A.C. 69, 123-126 (1986). TLC of CPA and aflatoxins on silica. CPA on silica impregnated with 2 % ethanolic oxalic acid solution with benzene - acetic acid - methanol 90:5:7. Visualization by spraying with 1 % p-dimethylaminobenzaldehyde in 75 ml ethanol and 25 ml HCl. Aflatoxin with anhydrous ether - methanol - water 96:3:1. Patulin and griseofluvin with toluene - ethyl acetate - 90 % formic acid 5:4:1. Detection of grimeofluvin under UV 366 nm, of patulin by spraying with 0.5 % aqueous solution of 3-methyl-2- benzothiazolinone hydrazone and heating to 130 °C. Assessment by visual comparison with standards.