J. Liq. Chromatogr. Relat. Technol. 37, 1805-1818 (2014). HPTLC of azithromycin dehydrate (1) and cefixime trihydrate (2) on silica gel with methanol – ethyl acetate – toluene – ammonia (30 %) 45:35:20:4. Quantitative determination (A) by absorbance measurement at 366 nm or (B) after spraying with stannic chloride reagent (40 mL chloroform and 40 mL glacial acetic acid were mixed and 5 mL of stannic chloride was slowly added) or (C) ethanolic sulfuric acid (20 mL of concentrated sulfuric acid in 80 mL of ethanol) followed by absorbance measurement at 450 nm for (B) and (C). The hRF values for (1) and (2) were 80 and 27 for (A) and (B) and 67 and 30 for (C). Due to concentrated sulfuric acid, (C) showed a slight change in hRF. Linearity for (1) was in the range of 1000-5000 ng/zone (A), 1500-5000 ng/zone (B) and 1000-6000 ng/zone (C) and for (2) was 400-2000 ng/zone (A), 1600-4000 ng/zone (B) and 800-2400 ng/zone (C). The intermediate/inter-day/intra-day precision was below 2 % (n=3). The LOD and LOQ for (1) was 85 and 257 ng/zone (A), 119 and 360 ng/zone (B) and 39 and 119 ng/zone (C) and for (2) it was 36 and 110 ng/zone (A), 194 and 588 ng/zone (B) and 69 and 208 ng/zone (C), respectively. Recovery was in the range of 98.2-102.4 % for both (1) and (2).

J. Planar Chromatogr. 29, 229-231 (2016). HPTLC of imipenem, meropenem, doripenem and eratapenem on silica gel G with 11 developing systems. Detection by spraying with iodine solution (0.2 g of potassium iodine and 0.4 g of iodine_x000D_ mixed in 20 mL of ethanol and 5 mL of hydrochloric acid), ninhydrin reagent (0.1 % ninhydrin in ethanol) or potassium permanganate solution (1 mg dissolved in 10 mL of water). The hRF values for the studied carbapenems in each developing system ranged between 13 and 58.

J. Liq. Chromatogr. Relat. Technol. 41, 315-323 (2018). HPTLC of three types of macrocyclic compounds, i.e. (1) cyclodextrins, (2) calixarenes and (3) macrocyclic antibiotics, on silica gel or RP-18 with different compositions of water – organic liquids (methanol, ethanol, acetonitrile). Detection of (1) on silica gel with a 1:1 mixture of 10 % α-naphthol in ethanol and 10 % sulfuric acid in water, followed by heating at 120 °C for 5-10 min; and on RP18 W by spraying with a 25 % methanolic solution of sulfuric acid, followed by heating at 120 °C for 5-10 min. Detection of (2) by spraying with 20 % aqueous solution of perchloric acid, followed by heating at 120 °C for 5 min. A comprehensive retention data set was obtained for fast selection of mobile phase compositions.

of Medicine Industry 12, 30-31 (1983) (Chinese) (Screening of antibiotics by thin-layer chromatography and bioautography.) TLC of antibiotics on silica with chloroform - methanol - NH3 in different proportions. Detection by overlaying the plate with agarose gel containing neutral red, protein, beef extract, NaCl, glucose and test bacteria, e.g. Escherichia coli, and incubating at 37 °C for 18-24 hr: red bacteria areas and yellow inhibition zones.

J. Microchem. 33, 209-215 (1986). TLC of daunomycin hydrochloride, adriamycin hydrochloride and their combination with dimethylsulfoxide and ascorbic acid on HPTLC silica with butanol - acetic acid - water 4:1:5 and propanol ethyl acetate - water 7:1:2. Detection by UV 254 nm.

J. Chromatogr. 403, 343-349 (1987). TLC on silica with 1. ethyl acetate - methanol - 25 % NH3 85:10:5, 2. ether - methanol - 25 % NH3 90:9:2, 3. dichloromethane - methanol - 25 % NH3 90:9:1.5, and 4. diisopropyl ether - methanol - 25 % NH3 75:35:1. Detection by drying at 110 °C for 5 min., spraying with 4-methoxybenzaldehyde - sulfuric acid - ethanol 1:1:9 and heating again for 1 min.

J. Chromatogr. 490, 395-403 (1989). TLC on silica with ethanol - water 85:5. Fluorescence enhancement by spraying with acetic acid solution and dipping into paraffin. Quantification by densitometry. Detection limit, 1 ng/mL.

Phytochemical Analysis 2, 199-203 (1991). TLC of antifungal compounds and crude plant extracts on silica with chloroform - methanol 7:3; HPTLC on diol with chloroform - methanol 98:2 and on RP-18 with chloroform - methanol 8:2. After development, the inocolum of agar with Candida albicans culture was distributed over the plate at 35°C. The bioautograms were sprayed after 12h incubation with an aq. solution of thiazolyl blue (2.5mg/mL). Clear inhibition zones can be observed against a purple background. Method also suitable for the search for antibacterial compounds as shown with Bacillus subtilis as test organism.