Proc. 9th Internat. Symp. Instr. Chromatogr., Interlaken, April 9.-11., 69-73 (1997). HPTLC of neomycin B, C and neamin (neomycin A) on silica with 1. methanol - acetone - 5% acetic acid 12:15:13 and 2. methanol - acetone - 25% NH3 12:15:13. Postchromatographic derivatization with fluorescamine, stabilization with paraffin/triethanolamine/methyl ethyl ketone.

Part IV: Quantitation and evaluation of penicillins. J. Planar Chromatogr. 12, 180 - 185 (1999). TLC of ampicillin, piperacillin, penicillin, mezlocillin, ticarcillin and oxacillin (direct sample determination) on RP hydrocarbon-impregnated HPTLC plates without solvent elution. The samples remained as a single spot centered about the point of application thereby facilitating direct quantitation by densitometry at different wavelengths. Detection limit 0.1 ng. Quantitation by densitometry in the range of 200 to 300 nm. This technique, direct sample determination (DSD), results in a higher sample throughput in reduced time with all samples being determined under the same conditions as the standards, without sample elution.

J. Liq. Chrom. & Rel. Technol. 25, 1579-1587 (2002). TLC and HPTLC of flumequine (9-fluoro-6,7-dihydro-5-methyl-1-oxo-1H,5H-benzo[ij]quinolizine-2-carboxylic acid) on silica gel with 0.05 M citric acid - methanol -2-propanol 1:3:1 or chloroform - acetone 9:1. Bioautography was performed by immersing the developed TLC plates briefly in the microorganism solution and incubated overnight at 28°C. Then the plates were sprayed with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT)-solution and incubated for about 30 min. Yellow inhibition zones were seen against a purple background. Sensitive, simple and cheap separation and determination procedure.

J. Planar Chromatogr. 19, 104-108 (2006). HPTLC and TLC of ciprofloxacin and enrofloxacin on silica gel with dichloromethane - methanol - 2-propanol - 25% aqueous ammonia 3:3:5:2. The plate was developed in a DS chamber to the top and the separation chamber was then uncovered for about 1 cm to enable the solvent to evaporate. In this way the plate was developed continuously for 2 h. Bioautography by immersion of the plate in a microorganism solution (Bacillus subtilis), incubation for 22 h at 37 °C. Visualization by spraying with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) solution and leaving for approximately 30 min at room temperature.

Quim. Nova 33, 43-47 (2010). HPTLC of aflatoxin B1 in peanuts on silica gel with chloroform - acetone 99:1. Quantitative determination by absorbance measurement at 366 nm, using a CCD camera followed by image processing using the software ImageJ. Linearity was between 0.8 and 4.8 ng/zone. The intra-day and inter-day precisions had a RSD lower than 5.2 %. LOD was 0.4 ng/zone while LOQ was 1.2 µg/kg. The average recovery was 94.9 %. The proposed method is a simple, efficient and low cost tool for quantitative analysis of aflatoxin B1 in peanut samples.

Food Control. 72, 110-122 (2017). Review of the methodologies reported on mycotoxin analysis in Sub-Saharan Africa. The review highlights the analytical methods reported for monitoring of toxic contaminants in food and feedstuffs, including references on the application of TLC and quantitative densitometry. _x000D_

J.A.O.A.C. 67, 580-582 (1984). HPTLC of zearalenone and zearalenol on silica with a) chloroform - ethanol 95:5 and b) benzene - acetone 9:1. Detection by spraying with 0.7 % Fast Violet B solution, followed by borate buffer solution. Sensitivity > 80 ng/g for zearalenone and > 200 ng for zearalenol.

J.A.O.A.C. 68, 136-137 (1985). TLC of aflatoxins on silica with chloroform - acetone 9:1in the first dimension and ether - methanol - water 96:3:1 in the second. Assay by viewing under 360 nm UV light and comparing with standards at 1 to 2 ng per spot.