China. J. AOAC Int. 82, 657-662 (1999). TLC of aflatoxins on silica gel with toluene - acetone 1: 1; visualization under UV 365 nm. The limit of detection was 20 ng/g.

Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 343-349. HPTLC of aflatoxins (B1, B2, G1, G2, M1), ochratoxin A, patulin, deoxynivalenol, fumonisins, sterigmatocystin, cyclopiazonic acid and altenuene on silica gel with chloroform - acetone 22:3, benzene - methanol - acetic acid 18:1:1, toluene - ethyl acetate - formic acid 5:4:1, chloroform - acetone - 2-propanol 8:1:1, chloroform - methanol - water - acetic acid 55:36:8:1, acetonitrile - 2-propanol - 0.25 M phosphoric acid 4:5:10, ethyl acetate - 2-propanol - NH3 6:3:2 and toluene - ethyl acetate - formic acid 6:3:1, respectively. Detection under UV or by spraying with aluminium chloride, 4-methoxybenzaldehyde, trifluoroacetic acid - aluminium chloride, and 4-dimethylaminobenzaldehyde. Quantitation by densitometry at different wavelengths in absorbance or fluorescence mode.

Chem. Pharm. Bull. 30, 2860-2869 1982). TLC of phenolic antibiotics produced by basidionycete, asperugin and congeners on silica with benzene - ethyl acetate 9:1, chloroform - methanol 9:1, chloroform - methanol - acetic acid 90:10:3 or benzene - dioxan - acetic acid 90:25:4. Detection by UV. Also column chromatography.

J. Chromatogr. 261, 130-133 (1983). TLC of trichothecene mycotoxins, T-2, diacetoxyscirpenol, HT-2 toxin, T-2-tetraol, T-2-triol, vomitoxin on silica with toluene - ethyl acetate - 90 % formic acid 5:4:1. Detection by 1) spraying with aluminium chloride reagent, heating at 110 °C for 5 minutes or by 2) spraying with chromotropic acid reagent and heating at 110 °C for 5-15 minutes; subsequent visualization by UV 366 nm. Detection levels 50-100 ng per spot.

Acta Alimentaria 12, 249-263 (1983). TLC of toxin F-2 and metabolite on silica with a) chloroform - ethanol 97:3, b) chloroform - methanol 9:1, c) benzene - methanol 9:1, d) benzene - methanol - acetic acid 18:1:1, e) benzene - acetic acid 9:1. Detection by UV 254 and 366 nm or by spraying with 2 % of 4-methoxy-benzene - diazonium-fluoroborate + with 0.1 N alcoholic potassium hydroxide solution.

J. Liquid Chromatogr. 7, 1383-1391 (1984). HPTLC of aflatoxins B1, B2 and free fatty acids on silica with a) 8 % acetone in chloroform, b) chloroform - acetone - acetic acid 92:8:1. Detection by U'V. In situ fluorometry at 366/400 nm.

J. Planar Chromatogr. 5, 35-40 (1992). HPTLC of lasalocid sodium, monensin sodium, narasin sodium, salinomycin sodium on silica and RP-18 with ethyl acetate – water 100:3 resp. with methanol – 5% aqueous acetic acid 9:1 (2 x). Lipids (i.a. L-a-phospatidylcholine-ß-linoleoyl-y-palmitoyl, dipalmitoyl L-a-phosphatidylethanolamine, triolein, oleic acid, L-a-dipalmitoyl phosphatidylcholine) were chromatographed with cyclohexane – ether – acetic acid 90:10:1 on silica, with chloroform on RP-18. VPF resposes on silica and RP-18 have been compared for both classes of compounds: the respose of the polycyclic ether antibiothics narasin and salinomycin on RP-18 plates was three times greater than on silica; lipids with the exception of cholesterol could be detected only on silica plates. Quantification by fluoro densitometry at 366/>400 nm.

3. Test fungus: Candida albicans. J. Planar Chromatogr. 20, 385-389 (2007). Optimum conditions have been established for culture of the fungus Candida albicans for microbial detection of zones in direct bioautographic TLC. On the basis of the results with Candida albicans it can be differentiated between microbiostatic (bacteriostatic or fungistatic) and microbiocidal (bactericidal or fungicidal) effects on TLC plates. Aqueous solutions of amphotericin B and fluconazole were spotted on TLC plates coated with silica gel. After drying the plates were immersed in microbial suspensions with different optical densities. The viability and metabolic activity were evaluated by use of a bioluminescence ATP assay modified by Köszegi.