Biochemical Systematics and Ecology 14, 307-310 (1986). Isolation and purification of iridoid glucosides, cornoside acetate and glucose. TLC on silica with dichloromethane - methanol - water 70:30:3 for iridoid glycosides and toluene- acetone 7:3 for cornoside acetate. Detection with vanillin 3 % and sulfuric acid 1 % in ethanol as spray reagent, followed by heating at 110 °C for 5 min. Separation of glucose with acetonitrile - CS2 - water 85:5:10, spray reagent thymol 1 % and sulfuric acid 3 % in ethanol.

Merck Spectrum (Darmstadt) 2, 60-61 (1988). TLC separation of sugars (rhamnose, xylose, arabinose, galactose) on silica with acetonitrile - water 85:15. Detection by spraying with naphtoresorcinol - sulfuric acid. Spray reagent: 0.2 g naphthalene-1,3-diol dissolved in 10 mL ethanol with some drops concentrated sulfuric acid added cautiously. For optimum color development followed by heating to 120°C.

Chinese J. Herb Med. (Zhongcaoyao) 26, 189-190 (1995). TLC of D-mannitol on silica with (dioxan-isopentyl-acetate - isopropanol 5:15:5:2) - (isopropanol - ethyl acetate - water 54:7:8) - acetic acid 60:40:1. Detection by spraying with 0.015 mol/L potassium periodate in 0.12 mol/L HCl, and 0.5 % diphenylamine in ethanol. Quantification by densitometry at 296 nm.

Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 89-95. : A quick, low-priced evaluation system, consisting of a flat bed scanner and a suitable software, is demonstrated. As example lactose in animal feed is determined on silica gel.

J. AOAC Int. 87, 707-717 (2004). TLC of oligosaccharides (produced on hydrolysis of high molecular weight fructan with endo-inulinase) with fructose, glucose, kestose, and kestotetraose with n-propanol - ethanol - water 7:1:2. The plates were developed once, and spots were visualized by spraying the plates with 5 % sulfuric acid in methanol, followed by heating at 120 °C for 5 min.

Food. Res. Int. 102, 282-290 (2017). HPTLC of fructooligosaccharides in pure orange juice on silica gel with n-butanol – 2-propanol – water 10:5:4. Detection by spraying with a mixture of phosphoric acid (6.8 mL), urea (3 g) and ethanol (8 mL) in 100 mL of 1-butanol – water 8:1, followed by heating at 120 °C for 10 min. Quantitative determination by absorbance measurement at 450 nm.

JAOAC Int. 83, 675-678 (2000). TLC of mono- and disaccharides (with D(-)-fructose and D(+)-sucrose as standards) before and after hydrolysis - (30 min with 1 % oxalic acid in a boiling water bath) - on silica gel with acetonitrile-diluted phosphate buffer solution pH 5.5 17:3. Detection by immersion of the plate for 1 s into a solution of 2 mL aniline and 2 g of diphenylamine in 100 mL methanol and 15 mL o-phosphoric acid (85 %) and heating for 10 min at 120°C. Quantitation by densitometry at 560 nm in absorption/transmission mode. Improved and simple method for the determination of fructan inulin in yoghurts, honey cakes and chocolates.