Var. australis (Diels) Rehd.) (Chinese). Chinese J. Herb Med. (Zhongcaoyao) 24, 563-566(1993). Preparative TLC of saponins and monosaccharides on silica with the lower phases of a) chloroform - methanol - water 16:5:1, and b) a solution of 9 mL of the above mixture blended with 1 mL acetic acid. Detection by spraying with 50% sulfuric acid in methanol for saponins and with naphthyl alcohol - sulfuric acid reagent for saccharides. Identification by MS and NMR.

J. Planar Chromatogr. 18, 285-289 (2005). HPTLC of glucose - after hydrolyis of starch using alpha-amylase and amyloglucosidase - on silica gel, pre-washed with methanol, treated by immersion in a 0.1 M solution of di-potassium hydrogen phosphate in methanol and activated for 30 min at 120 °C. Three-fold development was performed in a horizontal development chamber with acetonitrile - water 17:3. Detection by dipping in aniline-diphenylamine reagent, densitometry at 520 nm. Calibration was linear between 100 and 300 ng with a coefficient of determination r2 of 0.9959. The limits of detection and quantification for starch as glucose were 0.26 and 0.51 g/100 g, respectively.

as a leading compound for quantification in raw material and finished product. J. Liq. Chromatogr. Relat. Technol. 33, 996-1004 (2010). TLC of xylose with acetonitrile - water 9:1 with chamber saturation at ambient temperature. Detection by dipping into 4-aminobenzoic acid reagent for 1 to 2 s followed by heating at 110 °C for 10 min. The hRf of xylose was 56. Quantitative determination by densitometric evaluation at 366 nm. The linearity determination coefficient was r2 = 0.9999. The recovery of xylose was between 102 and 106 %. The precision of a six-time preparation was 1.6 %.

J. Agric. Food Chem. 63, 9764-9769 (2015). HPTLC of the reaction products of 4-nitrophenyl β-D-glucoside (4NPGlc) with oleic acid, linoleic acid, and purified rice bran substrate catalyzed by rice transglucosidase Os9BGlu3 on silica gel with chloroform - methanol - 30 % ammonia 35:14:1. Detection by spraying with 10 % sulfuric acid in ethanol, followed by heating at 110 °C. The hRF value for the glucosyl conjugate was 53.

assay for cellulolytic enzymes. Anal. Biochem. 150, 264-272 (1985). TLC of the title compound on silica with ethyl acetate - water - methanol 8:3:4. The method facilitates analysis of the activity of cellulolytic enzymes. Also separation of cellooligosaccharides using other adsorbents, solvents and impregnants.

in the district of Belgrade. Herba Hungarica 27, 61-69 (1988). TLC of saccharose, inuline, glucose and fructose on silica with benzene - ethyl acetate 95:5. Detection with vanillin - sulfuric acid reagent, and heating at 105°C for 15 min.

Biochemical Systematics and Ecology 23, 213-222 (1995). TLC of carbohydrates on silica, impregnated with 0.5 M NaH2PO4 in water - methanol 3:1, with isopropanol - acetone - 0.1 M lactic acid 2:2:1.

J. Liqu. Chromatogr. 23, 2413-2417 (2000). HPTLC of maltose, glucose and trehalose on silica gel pre-impregnated with sodium bisulfite (0.1 M) and citrate buffer using triple development with acetonitrile - water 17:3 or ethyl acetate - acetic acid - methanol - water 12:3:3:2. Detection by spraying with 1-naphthol reagent and heating for 10 min at 110°C. Simple and sensitive method.