J. Chromatogr. 312, 492-496 (1984). TLC of oligo (D-galactosiduronic acids) obtained by hydrolysis of pectic acid with trichoderma reesei polygalacturonase on microcrystalline cellulose with ethyl acetate - butanol - formic acid-water 1:3:5:2. Detection by spraying with saturated aqueous solution of lead acetate or by dipping the plate in aniline phthalate reagent in acetone. One step development brought about the separation of the monomer and oligomers up to DP 5 within 1 h. With 3 runs oligomers up to DP 9 could be resolved.

J. Biochem. (Tokyo) 102, 291-296 (1987). Description of a method for immobilizing oligosaccharides on TLC plates for immunostaining. HPTLC of oligosaccharides on polyamide or amino-bonded silica and covalent linking to the plate by reductive amination with NaBH3CN. Detection by enzyme immunostaining using NeuGC-specific chicken anti-NeuGC-Lac Cer etc. Detection limit, 0.8 nmol of NeuGC-containing oligosaccharides.

J. Planar Chromatogr. 4, 77-79 (1991). HPTLC of xylose, 3-o-methylglucose and rhamnose on silica with ethyl acetate – pyridine – acetic acid – water 75:15;10:10 (3 consecutive runs); detection by immersion in amino-benzoic acid reagent. Detection by densitometry in reflectance mode at 400 nm. Determination of method accuracy for 0.4 and 0.8 µg/4 µL of each monosaccharides (mean results of 20 replicate analyses are reported); CV = < 2.7%.

Z. Lebensm. Unters. Forsch. A 206, 175-178 (1998). HPTLC of oligosaccharides (e.g. glucose, maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose) on silica gel with acetonitrile - 0.02 phosphate buffer 7: 3; the best resolution was achieved after the third development of the plate. Detection by dipping into diphenylalanine-aniline-phosphoric acid reagent, drying and heating to 120°C for 10 min. Densitometry.

CBS 82, 9-11 (1999). HPTLC-AMD of glucose, maltooligosaccharides, anhydrosugars, transglycosidation products, fructose and its derivatives, maltulose, Amadori-compounds and Heyns-compounds on silica gel. Caramelization: isocratic 23-step gradient based on chloroform - methanol - 0.05 % aqueous boric acid 20:11:2 with 3 mm increments of the developing distance. Maillard-reaction: isocratic 3-step gradient chloroform - dichloromethane - methanol - water 35:15:35:6 over 25, 50, 75 mm developing distance. Detection by dipping in aniline-diphenylamine-phosphoric acid reagent, followed by heating at 120 °C for 10 min. Quantification by absorbance measurement at 385 nm.

CBS 108, 9-11 (2012). HPTLC of fructose, galacturonic acid, rhamnose, xylose, and galactose in samples of agar agar, arabic gum, carubin, guaran, traganth, xanthan, pectin, alginic acid, alginates and carrageen on silica gel with i-propyl acetate - ethyl acetate - methanol - water 50:40:10:1 to a migration distance of 60 mm. Densitometric absorption measurement at 370 and 630 nm and peak area evaluation by linear or polynomial regression. Detection by immersion in aniline diphenylamine o-phosphoric acid reagent (20 % o-phosphoric acid (85 %) added to 1:1 mixtures of 2 % solutions of diphenylamine and aniline in acetone) and heating at 110 °C for 5 min. Comparison of HPTLC with GC gave comparable results, however, the HPTLC method is more effective in terms of sample throughput, robustness, costs and analysis time (8 times faster than GC).

Chinese J. Herb Med. (Zhongcaoyao) 25 (3), 128-129 (1994). TLC of polysaccharides on silica with 1) ethyl acetate - acetic acid - methanol - water 12:3:3:2, 2) chloroform - methanol - water 16:9:2. Detection by spraying with aniline - diphenylamine phosphate reagent. Quantification by HPLC.

Coll. czechoslov. chem. comm. 47, 2433-2439 (1982). TLC of oligogalacturonic acid on silica with ethyl acetate - pyridine - acetic acid - water 5:5:1:3. Detection with aniline-phthalate reagent and identification acc. to the Rf values as a function of polymerization degree.