J. Planar Chromatogr. 24, 491-496 (2011). HPTLC of pentose, hexose and disaccharides in pharmaceutical formulations on silica with aqueous micellar bile salt, sodium deoxycholate in acetonitrile 1:5 with chamber saturation. This mobile phase provided the best separation of the 22 phases tested. Detection by spraying with ethanolic orcinol solution.

J. Liq. Chromatogr. Relat. Technol. 39, 607-612 (2016). HPTLC of glucose, fructose, xylose, rhamnose, arabinose, and galacturonic acid in Adansonia digitata dry fruit pulp on diol phase with a 15-step gradient based on acetone - acetonitrile 1:1 and water mixtures for enzymatic degradation products. Monosaccharide moieties were separated on silica gel with acetonitrile - acetic acid - water 63:33:5 to a developing distance of 80 mm. Detection by dipping into 10 % sulfuric acid in ethanol. Quantitative determination by absorbance measurement at 400 nm. The hRF values for rhamnose and glucose were 69 and 52, respectively. The HPTLC metehod could rapidly analyze complex mixtures containing a broad variety of monosaccharides that overlapped in a HPLC method.

J. Agric. Food Chem. 34, 827-829 (1986). Separation of cyclitols (pinitol, monitol, sequoyitol, bornesitol, chiro-inositol, myoinositol, sorbitol, xylose, fructose, glucose, sucrose, maltose) on silica with water - ethyl acetate - 2-propanol 6:11:83. Detection with 0.5 % potassium permanganate in 1N sodium hydroxide solution. For specific detection of sugars a solution of 1 % aniline and 1 % diphenylamine in 100 ml acetone was mixed with 10 ml conc. sulfuric acid and sprayed before heating at 110 °C for 10 min.

J.Chinese Herb Med. (Zhongcaoyao) 21, 18 (1990). TLC of glycyrrhizin on silica with butanol - acetic acid water 4:1:2. Detection under UV 254 nm. Quantification by densitometry at 254 nm.

Diol layers. J. Planar Chromatogr. 10, 31-37 (1997). HPTLC of sugars (i.a. glucose, isomaltotetrose, isomaltotriose, isomaltose, raffinose, nystose, 1-kestose, lactose, lactulose, sucrose, galactose, fructose, arabinose, xylose, ribose, rhamnose) on diol with AMD using a fifteen-step ACN - water gradient with water concentration decreasing linearly from 35 to 15%. Detection by absorbance at 515 nm after derivatization with 4-aminobenzoic acid reagent or a-naphthol reagent by immersion for 2 min. After drying for 2 min finally heating at 100-120°C. Quantification by densitometry at 365 nm (fluorescence) and at 400 resp. 515 nm (absorbance).

J. Planar Chromatogr. 15, 449-453 (2002). HPTLC of D-(+)-glucose, D-(+)-galactose, and D-(+)-fructose on silica gel-NH2 with 1-propanol - nitromethane - water 5:3:2. Visualization under UV 366 nm followed by heating at 130 to 140°C and inspection under UV 366 nm.

Acta Chrom. 11, 102-107 (2001). The snail Biomphalaria glabrata is medically important because it serves as the intermediate host for the development and transmission of the human blood fluke parasite Schistosoma mansoni. The purpose of this study was to use HPTLC to determine the effects of S. mansoni larval infection on the maltose and glucose content of the hemolymph and digestive gland–gonad complex of B. glabrata. TLC on silica gel with pre-adsorbent zone and 19 channels, with ethyl acetate – acetic acid – methanol – water 13:3:3:2. Detection by spraying with 1-naphthol – sulfuric acid reagent, quantitative determination by absorbance measurement at 515 nm.

CBS 104, 13-15 (2010). HPTLC of aloeverose, glucose and galactose in aloe vera products on silica gel (impregnated with NaH2PO4) with acetone - iso-propanol - 0.1 M formic acid 2:2:1 (double development for products with high glucose content). Detection by immersion in 4-aminobenzoic acid reagent (1 g 4-aminobenzoic acid in 36 mL acetic acid, with 40 mL water, 2 mL 85 % phosphoric acid and 120 mL acetone), followed by heating at 100 °C for 10 min. Densitometric evaluation by fluorescence measurement at 366 nm. The limit of quantification was approx. 3 % in aloe vera products. RSD for polynomial calibration is 2.1 % for mannose in the range of 8-80 ng/band and 4.0 % using matrix calibration.