Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 89-95. : A quick, low-priced evaluation system, consisting of a flat bed scanner and a suitable software, is demonstrated. As example lactose in animal feed is determined on silica gel.

J. AOAC Int. 87, 707-717 (2004). TLC of oligosaccharides (produced on hydrolysis of high molecular weight fructan with endo-inulinase) with fructose, glucose, kestose, and kestotetraose with n-propanol - ethanol - water 7:1:2. The plates were developed once, and spots were visualized by spraying the plates with 5 % sulfuric acid in methanol, followed by heating at 120 °C for 5 min.

Food. Res. Int. 102, 282-290 (2017). HPTLC of fructooligosaccharides in pure orange juice on silica gel with n-butanol – 2-propanol – water 10:5:4. Detection by spraying with a mixture of phosphoric acid (6.8 mL), urea (3 g) and ethanol (8 mL) in 100 mL of 1-butanol – water 8:1, followed by heating at 120 °C for 10 min. Quantitative determination by absorbance measurement at 450 nm.

JAOAC Int. 83, 675-678 (2000). TLC of mono- and disaccharides (with D(-)-fructose and D(+)-sucrose as standards) before and after hydrolysis - (30 min with 1 % oxalic acid in a boiling water bath) - on silica gel with acetonitrile-diluted phosphate buffer solution pH 5.5 17:3. Detection by immersion of the plate for 1 s into a solution of 2 mL aniline and 2 g of diphenylamine in 100 mL methanol and 15 mL o-phosphoric acid (85 %) and heating for 10 min at 120°C. Quantitation by densitometry at 560 nm in absorption/transmission mode. Improved and simple method for the determination of fructan inulin in yoghurts, honey cakes and chocolates.

(Contribution to the analysis of starch hydrolysates.) Z. Lebensm. Unters. Forsch. 174, 23-28 (1982). HPTLC of malto-oligosaccharides on silica, twice with propanol - water - acetone 45:25:30 and once with propanol - acetone - water 50:40:10. Detection by dipping into a solution of 4 ml aniline + 4 g diphenylamine + 200 ml acetone + 30 ml o-phosphoric acid 85 % and heating for 15 minutes at 120 °C. Quantitative assessment

J. Chromatogr. 287, 391-398 (1984). HPTLC of various oligosaccharides on aminopropyl-bonded silica impregnated with monosodium dihydrogen phosphate with acetonitrile-water 7:3, 9:1 or 6:4. Detection with aniline-diphenylamine reagent. For the preparation of the plates, see L. Doner et al., Carbohydr. Res. 125, (1984). Impregnation was made by immersing the plates in a 0.2 M aqueous solution of the salt for 15 min.

Merck Spectrum (Darmstadt), Spec. Biochemistry, 78-79 (1989). TLC separation of raffinose, lactose, saccharose, glucose, fructose on silica (50000 Å pore width) with acetonitrile - water 17:3, visualization by dipping in aniline - diphenylamine solution (2 mg aniline + 2 g diphenylamine in 80 mL acetone, adding 15 mL o-phosphoric acid 85%, made up to 100 mL with acetone), followed by heating at 105°C for 15 min.

Carbohydr. Res. 251, 187-202 (1994). TLC of 1 - 15 oligomers of maltodextrins and isomaltodextrins and their branched dextrins on silica with acetonitrile - ethyl acetate - propanol - water 85:20:50:X, where X = 60, 60, 70 for maltodextrins and 90 and 100 for isomaltodextrins. Detection by dipping in a solution of 0.5% a-naphthol and 5 % sulfuric acid in ethanol, followed by heating at 120 °C. Quantification by densitometry. Detection limits 50 - 2000 ng. It was shown that glucose and each of the dextrins gave an identical response on a weight basis and that relative weight percents in a mixture could be obtained by dividing the density of each dextrin by the sum of the densities of the dextrins.