Biochemical Systematics and Ecology 22, 113-120 (1994). TLC of lichen substances on silica with toluene - ethyl acetate - formic acid 35:20:2. Detection under UV 254 nm.

Acta Chrom. 6, 7-12 (1996). HPTLC of fructose, glucose and sucrose on silica gel with concentration zone. Impregnation of the layer (except concentration zone) by spraying with 0.1 M sodium bisulfate solution, followed by drying and spraying with citrate buffer of pH 4.8. Three-fold development with acetonitrile - deionized water 17:3 after chamber saturation. Visualization by spraying with 1-naphthol - sulfuric acid reagent and heating at 110 °C. Quantification by densitometry at 515 nm.

J Food Sci Technol 46(2), 114-117 (2009). A method for isolation and analysis of the artificial sweetener sucralose from the Indian sweet Burfi has been developed. For the isolation samples were suspended in water, treated with K3Fe(CN)6 solution and zinc sulfate, and filtrated. Semi-quantitative HPTLC analysis on amino layer with acetonitrile - water 4:1. The developed plate was heated at 190 °C for 20 min and spots were detected under UV 365 nm. Treating the plate with 5 % methanol CTMA increased the sensitivity (25 ng/zone). Quantitative HPTLC on silica gel with dichloromethane - methanol 4:1. Detection by spraying with 15 % methanolic sulfuric acid and heating at 100 °C for 10 min. The spot density was evaluated with Bio-rad fluorescence imaging software and confirmed by comparison with the standard. The method was linear in the range of 0.5-1.25 µg/band.

CBS 116, 11-12 (2016). HPTLC of lactose in milk, yoghurt, and instant sauce on silica gel with acetonitrile – water 3:1 (with 0.1 % trifluoroacetic acid) to the migration distance of 5 cm. Detection by spraying with aniline-diphenylamine-phosphoric acid reagent (2 g diphenylamine and 2 mL aniline in 80 mL methanol, 10 mL of o-phosphoric aicd 85 %, filled up to 100 mL) and heating for 10 min at 120 °C. Evaluation under white light. Elution of zones from the underivatized plate into ESI-MS with acetonitrile – water 19:1 (with 0.1 % formic acid) and detection in positive ionization mode. The method is suitable for semiquantitative determination of lactose by image evaluation and allows for confirmation of lactose-free products as such.

Anal. Biochem. 150, 13-17 (1985). HPTLC of oligosaccharides on NH2 -bonded silica. Immunostaining with monoclonal antibodies. Detection by autoradiography. 10 pmol of lacto-N-difucopentaose I and lacto-N-fucopentaose III can be detected. The method may be applied to identify and characterize antibody-binding oligosaccharides librated from glyco-conjugates and detect the structural specificity of other carbohydrate-binding proteins.

J. Chinese Herb Med. (Zhongcaoyao) 20, 155-157 (1989). 2-Dimensional TLC on silica with a) toluene - ethyl acetate - methanol 5:5:4, b) toluene - ethyl acetate - methanol - formic acid 5:5:4:0.1, c) the lower layer of chloroform - methanol - water 15:6:2. Detection by immersing in aniline - o-phthalic acid - butanol 2:3:200. Study of the sequence of sugars on the chromatogram.

(HPTLC separation of low-molecular carbohydrates). Dünnschicht-Chromatographie InCom Sonderband 1996, 139-147. HPTLC of thermolysates of sugars (1,6-anhydroglucose, D-glucose, dextrin and starch) on silica with a two-step resp. a 23-step AMD development based on chloroform-methanol-water. Detection by spraying with aniline-diphenylamine-phosphoric acid and drying for 5 min at 105 °C. Quantification by densitometry at 385 nm.

J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32 (5), 404-407 (2001). TLC on cellulose layer with ethyl acetate - pyridine - acetic acid - water 5:5:1:3, before and after hydrolysis of uronic acid containing polysaccharides. Detection under UV and by spraying with aniline-O-phthalic acid reagent. Discussion of the advantages of the method compared to conventional TLC procedures.