Acta Pharm. Sinica (Yaoxue Xuebao) 22 (1), 70-74 (1987). HPTLC on silica after exposure to hydrogen chloride vapor for 20 min at 50-60°C with the lower layer of chloroform - methanol - water - acetic acid 30:12:4:5 with chamber saturation. Detection by immersion to a reagent consisting of 0.93 g aniline and 1.66 g o-phthalic acid in 100 mL butanol, followed by heating at 100°C for 15 min. Quantification by densitometry at 380 nm (absorbance). Correlation coefficients of rhamnose, arabinose and glucose were 0.998, 0.999 and 0.999, respectively. CV < 3%. The calibration curve was linear over the range of 0.1 - 1.0 mg/spot. A simple, rapid, sensitive and accurate method.

J. Planar Chromatogr. 24, 491-496 (2011). HPTLC of pentose, hexose and disaccharides in pharmaceutical formulations on silica with aqueous micellar bile salt, sodium deoxycholate in acetonitrile 1:5 with chamber saturation. This mobile phase provided the best separation of the 22 phases tested. Detection by spraying with ethanolic orcinol solution.

J. Liq. Chromatogr. Relat. Technol. 39, 607-612 (2016). HPTLC of glucose, fructose, xylose, rhamnose, arabinose, and galacturonic acid in Adansonia digitata dry fruit pulp on diol phase with a 15-step gradient based on acetone - acetonitrile 1:1 and water mixtures for enzymatic degradation products. Monosaccharide moieties were separated on silica gel with acetonitrile - acetic acid - water 63:33:5 to a developing distance of 80 mm. Detection by dipping into 10 % sulfuric acid in ethanol. Quantitative determination by absorbance measurement at 400 nm. The hRF values for rhamnose and glucose were 69 and 52, respectively. The HPTLC metehod could rapidly analyze complex mixtures containing a broad variety of monosaccharides that overlapped in a HPLC method.

J. Agric. Food Chem. 34, 827-829 (1986). Separation of cyclitols (pinitol, monitol, sequoyitol, bornesitol, chiro-inositol, myoinositol, sorbitol, xylose, fructose, glucose, sucrose, maltose) on silica with water - ethyl acetate - 2-propanol 6:11:83. Detection with 0.5 % potassium permanganate in 1N sodium hydroxide solution. For specific detection of sugars a solution of 1 % aniline and 1 % diphenylamine in 100 ml acetone was mixed with 10 ml conc. sulfuric acid and sprayed before heating at 110 °C for 10 min.

J.Chinese Herb Med. (Zhongcaoyao) 21, 18 (1990). TLC of glycyrrhizin on silica with butanol - acetic acid water 4:1:2. Detection under UV 254 nm. Quantification by densitometry at 254 nm.

Diol layers. J. Planar Chromatogr. 10, 31-37 (1997). HPTLC of sugars (i.a. glucose, isomaltotetrose, isomaltotriose, isomaltose, raffinose, nystose, 1-kestose, lactose, lactulose, sucrose, galactose, fructose, arabinose, xylose, ribose, rhamnose) on diol with AMD using a fifteen-step ACN - water gradient with water concentration decreasing linearly from 35 to 15%. Detection by absorbance at 515 nm after derivatization with 4-aminobenzoic acid reagent or a-naphthol reagent by immersion for 2 min. After drying for 2 min finally heating at 100-120°C. Quantification by densitometry at 365 nm (fluorescence) and at 400 resp. 515 nm (absorbance).

J. Planar Chromatogr. 15, 449-453 (2002). HPTLC of D-(+)-glucose, D-(+)-galactose, and D-(+)-fructose on silica gel-NH2 with 1-propanol - nitromethane - water 5:3:2. Visualization under UV 366 nm followed by heating at 130 to 140°C and inspection under UV 366 nm.

Acta Chrom. 11, 102-107 (2001). The snail Biomphalaria glabrata is medically important because it serves as the intermediate host for the development and transmission of the human blood fluke parasite Schistosoma mansoni. The purpose of this study was to use HPTLC to determine the effects of S. mansoni larval infection on the maltose and glucose content of the hemolymph and digestive gland–gonad complex of B. glabrata. TLC on silica gel with pre-adsorbent zone and 19 channels, with ethyl acetate – acetic acid – methanol – water 13:3:3:2. Detection by spraying with 1-naphthol – sulfuric acid reagent, quantitative determination by absorbance measurement at 515 nm.