J. Planar Chromatogr. 27, 444-448 (2014). HPTLC of quercetin in fresh tubers of Satyrium nepalense on silica gel with toluene – ethyl acetate – formic acid 7:5:1. Quantitative determination by absorbance measurement at 366 nm. The hRF value of quercetin was 54. Linearity was in the range of 2-12 μg/zone. The intermediate intra-day and inter-day precisions were below 1 % (n=3) for quercetin. The LOD and LOQ was 40 and 120 ng/zone, respectively. Mean recovery for quercetin was 99.8 %.

J. AOAC Int. 98, 857-861 (2015). _x000D_TLC direct bioautography of apigenin (1) and α-linolenic acid (2) in Matricaria recutita L. (chamomile) and Achillea millefolium L. (yarrow) tinctures on silica gel with chloroform - acetone 99:1. Bioautography by dipping into suspensions of Bacillus subtilis, S. aureus, S. epidermidis, Pseudomonas syringae pv. maculicola, Xanthomonas campestris pv., Escherichia coli and Aliivibrio fischeri for 10 s. In the case of soil bacteria and human pathogens, the plates were incubated at 37 °C for 5 h, followed by dipping into a solution of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 0.05 g/90 mL) for 5 s and further incubation at 37 °C for 2 h. The hRF values for (1) and (2) were 33 and 66, respectively.

J. Chromatogr. Sci. 54 (1), 64-69 (2016). Micro-TLC of 11 species of the Mentha genus and two finished pharmaceutical products in two-dimensional mode in normal (NP) and reversed-phase (RP) systems on cyano phase with non-aqueous eluents for NP-TLC and with mixtures of acetonitrile with water or methanol with water for RP-TLC. Optimization of one-dimensional systems was performed by determining RM values vs. composition of mobile phase dependencies for standards occurring in various Mentha sp. On the basis of these dependencies the most selective chromatographic systems for each run were chosen. Then most selective eluents were applied to optimize two-dimensional systems by creating Rf in NP systems vs. Rf in RP systems correlations. The best two-dimensional systems were chosen on the basis of R(2) values for Rf vs. Rf correlations (the lowest values of R(2) coefficients). Two-dimensional micro-TLC was used to separate phenolic compounds of the examined plant materials.

J. Planar Chromatogr. 31, 13-22 (2018). HPTLC of phenolic acids (chlorogenic acid, rosmarinic acid, protocatechuic acid, gallic acid, syringic acid, ellagic acid, trans-cinnamic acid, o-coumaric acid, m-coumaric acid, p-coumaric acid, caffeic acid, ferulic acid, sinapic acid) and some flavonoids (flavone, apigenin, luteolin, chrysin, quercetin, myricetin, kaempferide, kaempferol, hesperetin, naringenin, pinocembrin) on silica gel with n-hexane ‒ ethyl acetate ‒ formic acid 6:4:1. Detection at UV 280 nm and 330 nm. The method was combined with mass spectrometry to test the applicability on various complex matrix samples, such as propolis, roasted coffee, and rose hip crude extracts.

Planta Medica 83(14/15), 1149-1158 (2017). The cyclodextrane fractionation of the methanolic percolate of Salix reticulata aerial parts was analyzed on silica gel with ethyl acetate – methanol – water 100:13:10. Detection by derivatization with vanillin-sulfuric acid reagent. Luteolin and apigenin glycosides, catechin, procyanidins and phenolic glucosides (picein, triandrin, and salicortin) were isolated from these fractions through preparative HPLC._x000D_

Phytochemistry. 24, 1495- 1498 (1985). Two-dimensional TLC of extracts of phyllocladus species on cellulose with 1) t-butanol - acetic acid - water 3:1:1 and 2) acetic acid - water 6:94. Detection with vanillin-HCl reagent. Catechin, epicatechin and phylloflavan, a new flavanoid compound readily resolved.

Phytochemistry 25, 1719-1721 (1986).TLC of flavonoid sulfates on cellulose with butanol - acetic acid - water 3:1:1 or on polyamide with methanol - water -29 % NH3 10:9:1. Detection with 2-aminoethyldiphenylborinate (0.1 % in methanol). TLC of the hydrolysis products on polyamide with benzene - MEK - methanol 3:1:1. Also ion pairing HPLC. Specific analytical procedure for the detection of flavonoid sulfates in plant extracts.

Acta Pharm. Hungarica 57, 193-198 (1987). HPTLC of rutin, vitexin-4'- rhamnoside, hyperoside, vitexin and apigenin-7-glucoside on silica with ethyl acetate - formic acid - acetic acid - water 100:11:11:28 for glycosides, with toluene - ethyl acetate - acetic acid - water 30:50:16:4 for aglycones. Detection under UV 254 and 366 nm and spraying with 1% AlCl3 methanolic solution. Densitometry by absorbance at 400 nm.