and Vaccinium vitis-idaea. L. J. Planar Chromatogr. 13, 101-105 (2000). HPTLC of flavonoids (i. a. quercetin, avicularin, hyperin, isoquercetin) on silica gel by two- and three-step gradient elution with mixtures of toluene, hexane, ethyl acetate and methanol for glycosides and multiple development for aglycones. Videodocumentation was achieved by use of videoscanning equipment. Visualization under UV 254 or 365 nm; or by spraying with one of the three reagents: (i) 10% KOH in methanol; (ii) 1% solution of FeCl3; and (iii) Gibbs reagent. Quantitation by densitometry at 254 nm.

Planta med. 65, 671-673 (1999). Preparative TLC of e.g., 3,4-dihydro-5-hydroxy-7-methoxy-4- (4'-methoxyphenyl)coumarin on silica gel with dichloromethane - hexane - ethyl acetate 6:3:1, of 3,4-dihydro-4-(4'-hydroxyphenyl)-5,7-dihydroxycoumarin, 3,4-dihydro-5-hydroxy-7-methoxy- 4-(4'-hydroxyphenyl)coumarin, and 3,4-dihydro-5,7-dihydroxy-4-(4'-methoxyphenyl)coumarin on silica gel with 1. dichloromethane - hexane - methanol 6:3:1 and 2. dichloromethane - methanol 9:1. Detection under UV.

J. Planar Chromatogr. 15, 433-436 (2002). TLC of (+)-catechin, (-)-epicatechin and (+/-)-catechin on cellulose, preferably prewashed with water - acetic acid 19:1, with 1-butanol - water - acetic acid 4:2:1. Detection by dipping in anisaldehyde - sulfuric acid reagent and with vanillin - phosphoric acid reagent. The work shows the importance of optimization of prewashing, pre-conditioning, and use of the correct development chamber and detection reagent in the TLC analysis procedure.

with and without red heartwood. J. Planar Chromatogr. 17, 350-354 (2004). TLC of (+)-catechin and (-)-epicatechin on silica gel with diisopropyl ether - formic acid 9:1. Detection by spraying with vanillin-sulfuric acid reagent and heating at 120 °C for 5 min. Quantitative determination by absorbance measurement at 513 nm.

J. Planar Chromatogr. 19, 463-466 (2006). Preparative RP-HPTLC of anthocyanin extracts (e. g. malvidine) on silica gel with five-step gradient elution with different concentrations of toluene - acetonitrile - water - formic acid - n-butanol - 2-propanol and resp. addition of tert-butylmethyl ether. After extraction of the third zone from the layer, HPTLC on RP-18 as the best stationary phase with a three-step gradient elution using methanol - water - formic acid in different ratios. Quantitative determination by absorbance measurement at 470 nm.

International Journal of Pharma and Bio Sciences 1(1), 2-4 (2010). TLC of methanol 50 % extracts of cut dried flowers of Calendula officinalis on silica gel with chloroform – methanol 19:1. The hRf value of quercetin was 43. Quantitative determination by densitometry at 366 nm. The method was linear in the range of 1-5 µg/band. The identity of quercetin in the sample was confirmed by comparing hRf values and UV spectra of sample and standard.

J. Ethnopharmacol. 158, 454-457 (2014). The authors described how the European Pharmacopoeia provides a legal and scientific reference for the quality control of medicines. The document highlighted HPTLC as a tool for the screening of herbal drugs containing certain contaminants, such as aristolochic acids._x000D_

J. Planar Chromatogr. 29, 341-346 (2016). HPTLC of artemisinin in the seeds of Artemisia annua on silica gel with n-hexane ‒ ethyl acetate ‒ acetic acid 20:10:1. Detection by dipping into anisaldehyde reagent (glacial acetic acid ‒ sulfuric acid ‒ anisaldehyde 50:1:0.5) followed by heating at 110 °C for 5 min. Quantitative determination by absorbance measurement at 540 nm. The hRF value for artemisinin was 43. Linearity was between 200 and 1000 ng/zone. The intermediate precision was below 0.7 % (n=3). The LOD and LOQ were 30 and 80 ng/zone, respectively. Recovery ranged from 82.9 to 87.1 %.