Polish Journal of Pharmacology and Pharmacy 39, 55-61 (1987). TLC analysis of flavonoids, iridoids, phenolic acids and saponins in the lyophilized infusion obtained from flowers of Verbascum thapsiforme Schrad. Numerous separation systems and staining procedures.

J. Chinese Herb Med. (Zhongcaoyao) 23, 239-246 (1992). TLC of baicalein on silica with n-amyl alcohol – methanol – formic acid – water 7:1:1:1. Quantification by densitometry at 280 nm.

Biochemical Systematics and Ecology 23, 573-574 (1995). TLC of flavonoids as glycosides on silica with butanol - acetic acid - water 12:3:5 or chloroform - methanol - water 70:30:3.

Pharmazie 62, 803-812 (2007). TLC of anthocyanins in aronia, blueberry and lingonberry extracts and the respective preparations (e.g. capsules, lozenges, granulate) on silica gel with ethyl acetate - water - anhydrous formic acid 10:3:2 and 10:4:1. Detection in white light prior and after spraying with iron(III)chloride solution or with vanillin phosphoric acid reagent.

Food Chem. 146, 104-112 (2014). HPTLC-Vis-ESI-MS of major anthocyanins in powder berry extracts of bilberry, blueberry, chokeberry, açai berry and cranberry on silica gel with ethyl acetate - 2-butanone - water - formic acid 35:15:4:6 in the first run (for anthocyanins) and ethyl acetate - toluene - formic acid - water 50:15:6:4 in the second run merely for the cut upper plate part (for anthocyanidins). Quantitation by absorbance measurement using a 4-point calibration at 520, 530 and 555 nm (multiwavelength scan). Correlation coefficients were between 0.9988 and 0.9999. Repeatability of sample analysis (n=3) was below 3.6 %. For confirmation of the results or characterisation of unknown anthocyane zones, mass spectra were recorded. Chromatography was directly linked to the effect using DPPH* reagent and the luminescent Aliivibrio fischeri bioassay.The method demonstrated to be a rapid, visually appealing alternative to known HPLC methods.

Food Chem. 187, 460-468 (2015). HPTLC-direct bioautography of bioactive compounds in the extracts of cold-pressed hemp (1), flax (2) and canola (3) seed oil on silica gel with toluene - ethyl acetate - formic acid - water 15:30:5:3 for (2) and toluene - ethyl acetate - acetic acid 80:25:4 for (1) and (3). HPTLC-DPPH scavenging activity was determined by dipping into a methanolic DPPH solution, followed by drying for 90 s in the dark and heating at 60 °C for 30 s. The hRF values of dominant radical scavenging zones were in the range of 75-85 for (1), 70-90 for (2) and 64 and 95-100 or (3). HPTLC-antimicrobial Aliivibrio fischeri assay allowed the determination of major antimicrobial zones at hRF 40-49 and 55-66 or (1), 23, 45 and 60 for (3) and 95 for (2). Additional effect-directed analyses employing acetylcholinesterase (AChE) assay, planar yeast estrogen (pYES) bioassay and Bacillus subtilis bioassay as well as subsequent HPTLC-ESI-MS allowed targeted characterization of bioactive compounds.

J. Food Comp. Anal. 48, 25-33 (2016). HPTLC of kavalactones (K, DHM, DHK, M, DMY, Y) and flavokavins (FKA, FKB, FKC) in kava (Piper methysticum) on silica gel with hexane – dioxane 4:1. Individual standards of the following nine compounds of interest were applied on plates and scanned after elution to obtain their UV absorption maxima: flavokavin A (FKA, 361 nm), flavokavin B (FKB, 343 nm), flavokavin C (FKC, 368 nm), kavain (K, 247 nm), dihydromethysticin (DHM, 200 nm), dihydrokavain (DHK, 242 nm), methysticin (M, 306 nm), demethoxyyangonin (DMY, 338 nm), and yangonin (Y, 354 nm). Detection by dipping for 1 s into anisaldehyde – sulfuric acid reagent (10 mL sulfuric acid with 170 mL of ice-cooled methanol, 20 mL of acetic acid, and 1 mL of anisaldehyde reagent), followed by heating at 100 ºC for 3 min. Quality of the samples was assessed by computing two ratios after scanning the plates at 245 nm (kavain/total kavalactones; K/KL) and 366 nm (flavokavins/kavalactones; FK/KL). The ratio K/KL corresponds to the peak area of K versus the sum of the peak areas of all other kavalactones (DHM + DHK + M + DMY + Y). The ratio FK/KL corresponds to the sum of the peak areas of the three FK (A + B + C) versus the peak areas of Y and DMY._x000D_

J. Ethnopharmacol. 193, 490-499 (2016). HPTLC of quercetin in the leaves of Ocimum basilicum on silica gel with toluene – ethyl acetate – formic acid 17:11:2. Quantitative determination by absorbance measurement at 254 nm.