CBS 109, 2-4 (2012). Extraction of arabica coffee with 2 to 50 % robusta coffee by accelerated solvent extraction with tBME. A part was saponified with10 % ethanolic KOH solution for 2 h. HPTLC of coffee extracts and standards 16-O-methylcafestol (16-OMC) and 16-OMC esters on silica gel with toluene - ethyl acetate - acetic acid 93:7:1 for 16-OMC esters and with tBME - chloroform 1:1 for 16-OMC. Detection by spraying with vanillin sulfuric acid (1 g in 250 mL ethanol and 2 mL of sulfuric acid, prepared freshly) and heating for 1 min at 80 °C. Quantitative absorption measurement at 530 nm. The LOD was 163 ng/band. After saponification the LOD of free 16-OMC was 43 ng/band. Thus 2 % robusta can be detected in a coffee blend.

Planta Medica 82(4), 371-376 (2016). TLC was applied to compare acetone – water 4:1 extracts from the mycelia and medium of fungus Trichoderma koningiopsis YIM PH 30002; an ethyl acetate fraction of each extract was separated on silica gel (mobile phase unknown) and visualized by three methods: detection with iodine reagent, sulfuric vanillin reagent, and phosphomolybdic acid in ethanol (8 %, w/v); no difference was found. Both extracts were then combined for the further isolation on columns of koninginins A, B and F, koningiopisins A–H, and trichodermaketone C._x000D_

Planta Med 83(05), 412-419 (2017). For the purification of verbascoside, the ethanol-water fractionation through size exclusion chromatography (Sephadex LH-20) of an 80 % ethanolic extract of Lippia citriodora leaves was monitored by TLC on silica gel with chloroform – methanol 7:3, followed by detection with vanillin sulfuric acid reagent.

J. Liquid Chromatogr. 8, 2927-2947 (1985). HPTLC on silica and RP-18 silica with different solvents and solvent mixtures. Detection by UV.

J. of Natural Products 51, 804-805 (1988). TLC of swertisin, 2-O-rhamnosylswertisin, swertiajaponin and 2-O-rhamnosylswertiajaponin on cellulose with n-butanol - acetic acid - water 4:1:5 (upper phase), 5% acetic acid and chloroform - methanol - n-propanol - water 60:30:20:5.

Phytochemical analysis 1, 22-15 (1990). TLC of polar alkaloids from Schumaniophyton magnificum on silica impregnated with liquid paraffin with methanol – water 1:3, citrate buffer at pH 4.2 – methanol 3:1 or borate buffer at pH 10 – methanol 3:1, and of phenolic fatty acid esters from Buddleja species with chloroform – methanol – water 25:75:5. Also centrifugal thin-layer chromatography on paraffin coated plates for the isolation of phenolic fatty acid esters.

J. Planar Chromatogr. 4, 471-474 (1991). Analytical and preparative TLC on coumarins (e.g. imperatorin, isoimperatorin, oxypeucedanin, peucedanin) on silica with chloroform - ethyl acetate - chloroform - ethyl acetate - methanol stepwise gradient. Detection under UV 254 nm before and after spraying with 0.1% solution of iodine in KI and, separately, with a 5% methanolic potassium hydroxide solution. Preparative separation in specially made sandwich-type horizontal chambers (acc. to J.K. Ruminski).

J. Liquid Chromatogr. 17, 2737-2749 (1994). TLC on silica with methanol - chloroform 1:1. Detection by spraying with ninhydrin - collidin reagent. Quantification by densitometry at 200 nm for e-caprolactam before derivatization and 558 nm for e-aminocaproic acid after derivatization. Detection limits 200 µg and 20 µg, resp.