Chinese J. Food Sci. 29 (4), 291-295 (2008). HPTLC of celery extracts on silica gel with chloroform - methanol - water 360:46:7. Detection and identification of the flavonoids by the characteristic chemical reaction colors and the UV spectrum of the prepared samples. Quantitative determination of apigenin by absorbance measurement at 345 nm. The linearity was between 330 and 990 ng/zone. Precision ( % RSD) was 1.33 % (n = 5) and the recovery was 97.8 % (n = 5, RSD = 2.3 %).

Rev. Bras. Farma. 24, 330-340 (2014). TLC of hesperidin in the fresh fruits of Citrus sinensis on silica gel with n-butanol - acetic acid - water 4:1:5. Detection by spraying with methanol sulphuric acid (10 %), followed by heating at 110 ºC. Qualitative identification at UV 284 nm. The hRF of hesperidin was 61.

Journal of Pharmacognosy and Phytochemistry 4 (2), 1-4 (2015). HPTLC of petroleum ether and ethanol extracts of aerial parts of Blumea lacera D.C. (Asteraceae), collected in the Gorakhpur district, with chloroform - benzene - formic acid 15:5:3. Evaluation in daylight revealed 11 zones, at least 9 of them at hRf between 3 and 47. The ethanol extract was purified by washing with water, chloroform and ethyl acetate successively, and then by fractionation on a silica gel (60-120 mesh) column; the benzene subfraction yielded, after exposure to iodine vapor, one yellow zone (hRf 41), identified (by IR, NMR and MS) as 3,5-dihydroxy-3’-methyl-6,7,4’-trimethoxyflavanone.

Planta Medica 82 (16), 1438-1445 (2016). TLC on silica gel with chloroform – methanol 19:1, followed by detection under UV 254 nm and spraying with 10 % sulfuric acid with heating. TLC was used for 1) the ethyl acetate fraction of a hydromethanolic extract of Eryngium triquetrum aerial parts, and 2) for the monitoring of its fractionation on a cyclodextrin column, and of the fractionation of a selected fraction on silica gel column. The (chloroformic) first subfraction of the last step was found to contain only the polyyne alcohol falcarinol (panaxynol, carotatoxin) at hRF 90.

Planta Medica 82(18), 1576-1583 (2016). The fractionation (by column and flash chromatography) of an aqueous extract of the aerial parts of Hilaria hirsuta was monitored through TLC on silica gel with n-butanol – acetic acid – water 13:3:5. The compounds (including flavonoids narcissin and rutin, and two new saponins, herniariasaponins G and H) were detected with anisaldehyde sulfuric acid reagent.

J. Liq. Chromatogr. Relat. Technol. 41, 342-348 (2018). HPTLC characterization and classification of flavonoids in a set of 42 medicinal plants extracts commercially available in Romania on silica gel with ethyl acetate – toluene – formic acid – water 60:3:8:6. Detection by spraying with 2-aminoethyldiphenylborate solution 0.2 % in ethanol, after drying for 30 min at 60 °C. Qualitative identification under UV 254 nm and 365 nm. Plates were immersed into a DPPH solution (0.02 % in ethanol) and images captured with a CCD camera at between 5 and 30 min after plate immersion. A multivariate data analysis including Principal Component Analysis and Cluster Analysis was performed to classify the investigated medicinal plants according to their antioxidant capacity.

J. Ethnopharmacol. 232, 135-144 (2019). HPTLC of hesperidin in Citrus reticulatae pericarpium on silica gel with ethyl acetate – formic acid – acetic acid – water 15:1:1:2. Qualitative identification at UV 275 nm. The hRF value for hesperidin was 26.

Biochemical Systematics and Ecology 14, 79-80 (1986). Two dimensional TLC on polyamide with 1) water - butanol - acetone - dioxane 70:15:10:5 and 2) benzene - MEK - methanol - water 55:22:20:3. The air-dried chromatograms are sprayed with 0.1 % aminoethyl diphenyl borate. Identification of kaempferol, quercetin, isohamnetin. The two-dimensfonal chromatographic pattern of 37 plants examined were found similar.